Composition and method for treating banana tree cercosporiosis
A composition and mixture technology, which is applied in the field of cryptogenic diseases, can solve problems such as unusable, high viscosity, and high toxicity, and achieve good stability
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Embodiment 1
[0055] A first set of experiments were performed in the laboratory to screen for inhibitors of extracellular enzymes useful in this application. Experiments were carried out in Petri dishes to monitor the spread of the fungus P. Composition A containing 105 g / l boron complexed with 180 g / l alkanolamine and chelated with 180 g / l polyol and composition B containing 100 g / l boron complexed with 360 g / l ethanolamine were prepared. In addition, a mineral oil composed of 56% paraffins and 44% naphthenes was prepared. The mineral oil has a viscosity of 8.7 cSt at 40°C, measured according to the NFEN ISO 3104 standard of August 1996, and is composed of petroleum fractions obtained by distillation, for said distillation, between 10% and 90% The temperature range at which it distilled was 16°C and the middle distillation point at which 50% of the oil distilled was 197°C.
[0056] Composition 1 according to the invention comprises 30% by volume of composition A and 70% by volume of min...
Embodiment 2
[0068] A second experiment in liquid medium was performed using the same growth medium without agar. The medium was sterilized in an autoclave, and 1% by volume of Composition 1 or Composition 2 of the present invention used in Example 1 was added to the treated sample, respectively. A third treatment was prepared by adding 1% by volume of Composition 1 and 1 g / l EDTA (Composition 3) to the medium. This medium was placed in a closed sterile flask equipped with an electronic manometry device (OxiTop EC100). Two flasks were used for each treatment and two flasks were used as controls, ie they were not treated. All flasks were inoculated with Xanthomonas spp. and incubated at 25°C with a 12 / 12 photoperiod. A manometric device can measure the oxygen consumption in the flask. This oxygen consumption may be directly related to the growth of microorganisms.
[0069] The test results in liquid medium are as follows figure 2 as shown in figure 2 Denotes O in flasks containing v...
Embodiment 3
[0076] Field trials were also conducted in Cameroon. The trials were carried out for 4 months on a land area of 0.5 ha, using 130 banana plants per trial. Treatments were performed at 10-day intervals. The cultivar of the banana plants used was Grande Naine (Cavendish, AAA), which is extremely susceptible to black leaf spot. This test allows the effect of the composition of the invention to be compared with a negative control (no treatment) and two positive controls (two conventional fungicides). The contact fungicide Penncozeb 75DG (mancozeb) (dithiocarbamate) and the systemic fungicide Impulse 800EC (spiroxetalamines) (spiroketalamines) were used as positive controls .
[0077] Composition 4 of the invention comprised 1% by volume of Composition A of Example 1 (i.e. a composition containing 105 g / l boron complexed with 180 g / l alkanolamine and chelated with 180 g / l polyol) , 98% by volume of the mineral oil of Example 1 and 1% by volume of Triton X45. Mineral oil is t...
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