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Flow type electroporation device and system

A flow electroporation and electroporation technology, applied in biochemical equipment and methods, biochemical instruments, stress-stimulated microbial growth methods, etc., can solve problems such as destroying flow channels, reducing electric field uniformity, and reducing cell survival rates. Achieve the effect of improving conductivity, increasing processing speed and reducing equipment cost

Inactive Publication Date: 2013-04-03
PEKING UNIV
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  • Abstract
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AI Technical Summary

Problems solved by technology

However, in such systems, where the electrodes are in direct contact with the cell suspension, electrolysis of water inevitably occurs during electroporation of cells.
Accompanied by the electrolysis of water, a large amount of heat, air bubbles and drastic changes in the pH value of the cell suspension will significantly reduce the survival rate of the cells and destroy the uniformity of the electric field in the flow channel, thereby reducing the perforation efficiency of the cells

Method used

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  • Flow type electroporation device and system
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  • Flow type electroporation device and system

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Embodiment Construction

[0053] Below in conjunction with accompanying drawing and specific embodiment the present invention is described in further detail:

[0054] 1 Substrate:

[0055] Because the substrate acts as a support and acts as an insulation and isolation between electrodes, the substrate needs to be made of insulating material, or made of a non-insulating material covered with an insulating layer. The substrate according to the present invention can be made from any solid substrate suitable for the above conditions, preferably those substrates which can be molded or machine cut to the required specifications. Transparent glass or polymer is particularly preferred, since in this case the state of the cells can be observed in real time through a microscope.

[0056] exist figure 2 In the preferred embodiment shown, the substrate is made of ordinary glass, with a length of 8 cm, a width of 2.5 cm, and a height of 1 mm. The size of the substrate can be determined by specific requirements,...

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Abstract

The invention discloses a flow type electroporation device and a system, and the system comprises the flow type electroporation device, a channel, an injection pump and a voltage source, wherein the flow type electroporation device comprises a substrate and electrodes which are made on the substrate and are arranged in parallel and in pairs, and each pair of the electrodes comprise an anode and acathode which are oppositely arranged; the channel is arranged above the electrodes and limits fluid to flow; an initial end of the channel is provided with a plurality of inlet branch channels whichconverge into a main channel, a terminating end is provided with a plurality of outlet branch channels, and a plurality of head covers for fluid inlets and outlets are arranged above the channel; theinjection pump is connected to inlets and outlets of the head covers in the flow type electroporation device and limits the flow speed of the fluid; and the voltage source is connected with the electrodes through electrical connectors and sets and produces pulse voltage. The flow type electroporation system utilizes the fluid channel and the connected injection pump to realize continuous flow of various suspensions in the fluid channel, thereby enabling the process that cells are subjected to electroporation to be continuous and realizing rapid treatment of a large number of samples.

Description

technical field [0001] The present invention relates to a flow electroporation device, more specifically, the present invention relates to a system for efficiently using the flow electroporation device to electroporate cells. Background technique [0002] The cell membrane is a layer of thin film surrounding the periphery of the cell, which is a permeability barrier for the selective material exchange between the cell and the outside world. The cell membrane makes the cell an independent living unit and has a relatively stable internal environment. Some substances in the surrounding environment can pass through the cell membrane, while others cannot. Cells can absorb nutrients from the surrounding environment through the cell membrane, discharge metabolites, and make the transport of substances reach a state of balance. Therefore, the basic function of the cell membrane is to maintain the relative stability of the intracellular microenvironment and selectively exchange sub...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12M1/42
CPCC12M35/02
Inventor 李志宏梁子才魏泽文黄璜
Owner PEKING UNIV
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