Human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card

A colloidal gold and echinococcosis technology, applied in the field of medicine and biology, can solve the problems of lack of grassroots units, early detection, early diagnosis and early treatment of cases, etc., to improve sensitivity and specificity, reduce missed or misdiagnosed, and reduce cross-reactions Effect

Inactive Publication Date: 2011-01-19
ZHEJIANG UNIV
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Problems solved by technology

Community hospitals, epidemic prevention stations, medical schools and scientific research institutions need special scientific research equipment for serological, immunological and imaging diagnosis of the dis

Method used

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  • Human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card
  • Human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card
  • Human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card

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Embodiment Construction

[0014] The present invention is further described below by embodiment:

[0015] refer to figure 1 , figure 2 , Detection device: composed of an external plastic cartridge and a detection module inside, the external plastic cartridge includes a base 1 and a casing 2, and the casing 2 has a sampling hole 3 and a rectangular observation window 4; the internal detection module consists of a filter sample paper 5. Composed of nitrocellulose membrane 6 and absorbent paper 7. Colloidal gold-labeled anti-hydatid cyst fluid antigen human single-chain variable region antibody 8 is adsorbed on absorbent paper 7 and nitrocellulose membrane 6 to form a quality control line 9 and a detection line 10; filter sample paper 5 positive For the sample injection hole 3, the nitrocellulose membrane 6 is facing the observation window 4; the detection line 10 is marked by streptavidin; the quality control line 9 is marked by rabbit anti-mouse IgG.

[0016] Detection process: After the urine sampl...

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Abstract

The invention discloses a human echinococcosis colloidal gold immunochromatographic assay urine testing quick diagnosis test paper card. The test paper card comprises a plastic card box and a detection module in the card box; the plastic card box is provided a sample loading hole and an observation window; the detection module comprises sample filtering paper, a nitric acid fibrous membrane and absorbent paper; echinococcosis resistance fluid antigen humanized single chain variable region antibody which is marked by colloidal gold is absorbed on the absorbent paper and nitric acid fibrous membrane; a quality control line and a detection line are marked on the echinococcosis resistance fluid antigen humanized single chain variable region antibody marked by the colloidal gold on nitric acid fibrous membrane; the sample filtering paper is opposite to the sample loading hole; and the nitric acid fibrous membrane is opposite to the observation window. The test paper card has the advantages of simplicity and convenience, fastness, no invasiveness, reliability and stability.

Description

technical field [0001] The invention relates to the field of medical and biological technologies, in particular to a urine test paper for rapidly diagnosing human echinococcosis, which can quickly diagnose whether the echinococcosis is infected by using the color change observable by naked eyes. Background technique [0002] As a zoonotic disease that seriously endangers human health, echinococcosis has the characteristics of insidious onset, slow development and frequent recurrence, which requires early diagnosis and regular detection. In view of the severe prevalence of zoonotic diseases in my country, the Ministry of Health proposed in April 2007 the goal of "reaching 60% population coverage in high-incidence areas in the mission of echinococcosis epidemiological investigation". Community hospitals, epidemic prevention stations, medical schools and scientific research institutions need special scientific research equipment for serological, immunological and imaging diagno...

Claims

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Application Information

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IPC IPC(8): G01N33/569G01N33/558G01N33/532
Inventor 陈新华庄杰陈新梅殷胜勇蒋建文谢海洋周琳郑树森
Owner ZHEJIANG UNIV
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