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Quantitative detection method for salmonellas in municipal surplus sludge

A quantitative detection method and technology of excess sludge, applied in the direction of microbial measurement/inspection, biochemical equipment and methods, and resistance to vector-borne diseases, etc., can solve problems such as few reports of technical means, and achieve obvious selective effect, Avoid the interference of a large number of bacteria, which is conducive to the effect of accurate and rapid quantification

Inactive Publication Date: 2011-03-30
JIANGNAN UNIV
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Problems solved by technology

At present, there are only a few patent applications for the quantitative detection method of Salmonella, such as a rapid quantitative detection method for Salmonella in soil invented by the Nanjing Soil Institute of the Chinese Academy of Sciences (Patent Publication No. CN 101613744A), but for the quantitative detection of Salmonella in urban surplus sludge Relevant technical means are seldom reported

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  • Quantitative detection method for salmonellas in municipal surplus sludge
  • Quantitative detection method for salmonellas in municipal surplus sludge
  • Quantitative detection method for salmonellas in municipal surplus sludge

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Embodiment Construction

[0027] To determine the number of Salmonella in the residual sludge before and after dehydration in the secondary sedimentation tank of a sewage treatment plant in Wuxi, Jiangsu, the specific steps are as follows:

[0028] (1) Gradient dilution of sludge samples and pre-enrichment culture: Weigh 5g of the remaining sludge before and after dehydration and add it to a 250mL Erlenmeyer flask filled with 45mL of buffered peptone water (BPW), vortex and shake for 10min to mix evenly , Take 0.5mL of the mixed mud-water mixture and add it into a test tube containing 4.5mL of buffered peptone water to obtain each dilution gradient in turn. Set up 6 dilutions, and set three replicates for each dilution, and incubate at 36°C for 8 h.

[0029] (2) Selective enrichment culture: Pipette 0.1mL of the bacterial solution in each tube of enrichment before serial dilution and add it to a test tube containing 5mL of sodium tetrasulfonate brilliant green enrichment solution (TTB), and incubate...

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Abstract

The invention relates to a quantitative detection method for salmonellas in municipal surplus sludge, belonging to the technical field of pathogenic bacterium detection of municipal surplus sludge in the biotechnology. The detection method comprises the following steps of: (1) carrying out gradient dilution of a sludge sample and bacterial enrichment cultivation; (2) carrying out selective enrichment cultivation; (3) separating and identifying a color developing culture medium; and (4) calculating by using MPN (Most Probable Number) counting software to obtain a result. The invention combines the MPN method and a salmonella detection method in the national standard of food safety, not only meets the requirement on the detection of salmonellas in the municipal surplus sludge, but also avoids interference of a large quantity of mixed bacteria in the sludge on the basis of obvious selectivity effect and is beneficial to the quick quantifying of the salmonellas. The invention has important theoretical values and actual guide meanings in the aspects of formulating and improving municipal surplus sludge treatment and application standards, and the like.

Description

technical field [0001] The invention relates to a quantitative detection method for Salmonella in urban surplus sludge, which belongs to the technical field of pathogenic bacteria detection of urban surplus sludge in biotechnology. Background technique [0002] Salmonella belongs to Enterobacteriaceae, a gram-negative enteric bacillus, and the feces of people infected with Salmonella or carriers can contaminate food and cause food poisoning after eating. According to statistics, among all types of bacterial food poisoning in various countries in the world, food poisoning caused by Salmonella often ranks first. Salmonella is also the first bacterial food poisoning in my country's inland areas. On August 17, 2010, the California State Health Department announced that a Salmonella outbreak had occurred in several areas of California, and 266 cases of illness had been reported since June. Preliminary investigations showed that most patients contracted the disease after eating ...

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Application Information

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IPC IPC(8): C12Q1/10C12Q1/06
CPCY02A50/30
Inventor 刘和符波陈燕王燕
Owner JIANGNAN UNIV
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