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Establishing method of PPHN (Persistent Pulmonary Hypertension of Newborn) animal model

A pulmonary arterial hypertension and animal model technology, applied in animal husbandry and other directions, can solve the problem of no report on animal models, and achieve the effect of strong reproducibility, short time period, and reliable construction

Inactive Publication Date: 2011-05-04
ZHEJIANG UNIV
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Problems solved by technology

[0006] Models induced by hypoxia or indomethacin alone have been studied, however animal models co-induced by hypoxia combined with indomethacin have not been reported

Method used

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  • Establishing method of PPHN (Persistent Pulmonary Hypertension of Newborn) animal model
  • Establishing method of PPHN (Persistent Pulmonary Hypertension of Newborn) animal model
  • Establishing method of PPHN (Persistent Pulmonary Hypertension of Newborn) animal model

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Embodiment 1

[0020] 1. Establishment of animal models

[0021] Female SD rats weighing 250-300 g were mated overnight (counted as day d0), and the second day was marked as the first day of pregnancy (d1). The gestation period of rats in this model is 21 days. On the 19th day of pregnancy, pregnant rats were randomly divided into four groups to start hypoxia treatment, namely hypoxia group, indomethacin group, hypoxia and indomethacin combined treatment group and control group. Group. All treatments lasted 3 days.

[0022] Hypoxia group: put pregnant d19 rats in a plexiglass box with 12% oxygen concentration for 3 consecutive days. Hypoxic device see figure 1 , is to pass the nitrogen (about 87%) in the nitrogen bottle 2 and the oxygen (about 12%) in the oxygen bottle 3 to a plexiglass box 1 with two small holes, so that the oxygen in the plexiglass box 1 The concentration is maintained at about 12%. Soda lime and anhydrous calcium chloride (each 20-30g / day, replaced every day) are plac...

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Abstract

The invention relates to an establishing method of a PPHN (Persistent Pulmonary Hypertension of Newborn) animal model. The method comprises the following steps of: nurturing a pregnant animal under a hypoxic condition; and treating the pregnant animal by adopting indomethacin twice a day for 3 days, wherein the treatment amount is 0.5 mg / kg, and the oxygen concentration under the hypoxic condition is smaller than 20 percent. Because of applying a hypoxic detection system and injecting the indomethacin in the abdominal cavity, a PPHN rat model of the invention is more accurate and stable to construct and has stronger replicability, and BNP (Brain Natriuretic Peptide) is applied to indirectly measure the pulmonary artery tension of newborn rats so that the model is more reliable to construct. Relative to the construction process of a PPHN model of a large-size animal, the rat PPHN model is easy to establish, has short time period, low cost, convenience, stability and reliability and is suitable for lab researches and applications.

Description

technical field [0001] The invention belongs to the biological field and relates to a method for establishing an animal model of neonatal persistent pulmonary hypertension. Background technique [0002] Persistent pulmonary hypertension of the newborn (PPHN) refers to the continuous increase in pulmonary vascular resistance after birth, and the pulmonary artery pressure exceeds the systemic arterial pressure, which makes the transition from fetal circulation to normal "adult" circulation difficult, resulting in The right-to-left shunt of blood at the level of the foramen ovale and (or) ductus arteriosus, clinically manifested as severe hypoxemia, is more common in full-term and post-term infants. PPHN is an important clinical problem in neonatal intensive care units, and its incidence can be as high as 7 / 1000 newborns. Although mechanical ventilation, inhaled nitric oxide (NO) and extracorporeal membrane oxygenation have improved the prognosis of PPHN, the mortality rate is...

Claims

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Application Information

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IPC IPC(8): A01K67/027
Inventor 杜立中徐雪峰
Owner ZHEJIANG UNIV
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