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Application of dihydroartemisinin to preparation of tumor cell autophagy induction medicament

A technology of dihydroartemisinin and tumor cells, which is applied in the application field of dihydroartemisinin in the preparation of drugs that induce tumor cell autophagy, can solve problems such as death, cell necrosis, and unclear molecular mechanisms, and reduce inflammation Response, inhibition of tumor growth, reduction of local angiogenesis and nutrient supply

Inactive Publication Date: 2011-05-04
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Third, the initiation of autophagy will lead to cell death due to necrosis
It shows that the antimalarial activity and antitumor activity of artemisinin drugs are basically the same in mechanism of action, both of which are related to the destruction of the membrane structure on the organelle, but the exact molecular mechanism is not clear

Method used

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  • Application of dihydroartemisinin to preparation of tumor cell autophagy induction medicament
  • Application of dihydroartemisinin to preparation of tumor cell autophagy induction medicament
  • Application of dihydroartemisinin to preparation of tumor cell autophagy induction medicament

Examples

Experimental program
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Effect test

Embodiment 1

[0019] Example 1: Experimental research on autophagy induced by dihydroartemisinin in multiple myeloma cells

[0020] Experimental materials: Dihydroartemisinin raw materials were provided by Zhejiang Huali Nanhu Pharmaceutical Co., Ltd. The human multiple myeloma RPMI8226 cell line was donated by the Institute of Hematology, the First Affiliated Hospital of Zhejiang University. DMSO, Sinopharm Chemical Reagent Factory. Calf serum was purchased from Hangzhou Sijiqing Biological Engineering Co., Ltd. Polylysine, Boster Bioengineering Co., Ltd. Monodansyl pentadiamide (MDC), Fluka Corporation. Fluorescent inverted microscope, Leica-DMIL. Transmission electron microscope, Philips-TECNAI10. Flow Cytometry (FACS Caliur), Becton Dickinson.

[0021] Experimental method: RPMI8226 cells were cultured in PRMI 1640 culture medium containing 10% calf serum, 100U / ml penicillin and 100mg / L streptomycin at 37°C, saturated humidity, 5% CO 2 / 95% air (normal oxygen) incubator to observe...

Embodiment 2

[0044] Example 2: Experimental treatment of dihydroartemisinin on multiple myeloma cell model transplanted in SCID mice

[0045] Experimental materials: The raw material of dihydroartemisinin was provided by Zhejiang Huali Nanhu Pharmaceutical Co., Ltd., and it was prepared into 0.5% CMC-Na suspension before use. The human multiple myeloma RPMI8226 cell line was donated by the Institute of Hematology, the First Affiliated Hospital of Zhejiang University. Female SCID mice, 5-6 weeks old, weighing 18-22 g, were provided by the Shanghai Experimental Animal Center of the Chinese Academy of Sciences. Fluorescent inverted microscope, Leica-DMIL. Transmission electron microscope, Philips-TECNAI10.

[0046] experimental method:

[0047] 1. Experimental Treatment of SCI D Mice

[0048] SCI D mice, female, 5-6 weeks old, 18-22g, were raised in an SPF laboratory. Take logarithmic phase cell RPMI 8226 and dilute it to 2×10 with normal saline 8 ml -1 For cell suspension, inject 0.2m...

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Abstract

The invention provides application of dihydroartemisinin to preparation of a tumor cell autophagy induction medicament. The molecular formula of the dihydroartemisinin is C15H24O5. The medicinal preparation comprises a preparation-allowable medicinal excipient or carrier. The excipient is in the form of a solid preparation. The medicinal preparation provided by the invention can be used as a mitochondrion targeting autophagy inductor, which has a specific anti-tumor effect and can provide a therapeutic medicament for overcoming medicament resistance and improving prognosis for treatment of tumors. The effects and the mechanisms of effective monomer components in a traditional Chinese medicine are research and described under the background of a cell autophagy theory; and an important basis is provided for the development of new theory of the traditional Chinese medicine and new acting target of the medicament, and a new research direction for developing the theory of the traditional Chinese medicine is provided.

Description

technical field [0001] The invention belongs to the medicinal use, and relates to the medicinal use of dihydroartemisinin in the aspect of preparing and inducing tumor cell autophagy. Background technique [0002] Autophagy is an important and highly conserved mechanism in the evolution of nucleated organisms. It is a regulatory mechanism that relies on the lysosome pathway to decompose intracellular proteins or organelles to maintain the stability of the intracellular environment. There are changes in autophagy activity in a variety of human tumors, similar to programmed cell death, autophagy is considered to be an important mechanism for maintaining the stability of the intracellular environment and the development of multicellular organisms, and is called type II apoptosis. In mammalian cells, there are still many unknown steps in autophagy, especially how tumor cells initiate autophagy under hypoxic conditions and the targets of drug-induced autophagic apoptosis have bec...

Claims

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Application Information

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IPC IPC(8): A61K31/357A61P35/00A61P35/02
Inventor 周慧君吴秀华李菌张佳丽何玮欣薛占成
Owner ZHEJIANG UNIV
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