Cow recessive endometritis early diagnosis kit
A technology for endometritis and early diagnosis, which is applied in the field of immunological testing and can solve the problems of difficult control of semen temperature, difficult operation, and difficult sample collection.
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Embodiment 1
[0042] Embodiment 1: a kind of milk cow recessive endometritis early diagnosis kit of the present invention, it is to utilize the standard antibody of SAA, IL-6 and IL-8 to coat the microtiter plate, make competing antibody with novel monoclonal antibody, Use horseradish peroxidase to label the secondary antibody, prepare serum dilution solution, washing solution, substrate solution A, substrate solution B, H 2 o 2 , stop solution, assembly composition;
[0043] 1. Preparation of SAA, IL-6 and IL-8 antibodies
[0044] (1) Antigen acquisition and animal vaccination
[0045] Prokaryotic culture of E.coli, separated and purified by Ni column, and then multi-point intradermal injection of antigen emulsified with Freund's complete adjuvant on the back of the rabbit, each point needs to inject about 0.1ml, a total of 0.5ml injection, 2 Weekly booster immunization;
[0046] (2) Collection and storage of antiserum: Blood is collected from the ear vein or ear artery, the serum is s...
Embodiment 2
[0071] Embodiment 2: The following explanations are made on the relevant issues of the recessive endometritis competition ELISA detection kit:
[0072] 1 About the collection and preservation of specimens
[0073]Most ELISA tests use serum as a sample. In addition to still containing fibrinogen and anticoagulant in plasma, other components are equal to serum. Except for special circumstances, serum is used as the test specimen in medical tests. Serum and plasma can be used equally in ELISA. Serum samples can be collected according to conventional methods, and attention should be paid to avoid hemolysis. Substances with peroxidase activity will be released when red blood cells are lysed. In the ELISA assay using HRP as a marker, hemolysis samples may increase non-specific color development.
[0074] Serum samples should be tested when they are fresh. If there is bacterial contamination, the bacteria may contain endogenous HRP, which will also produce false positive reaction...
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