Development of herbicide-resistant grass species

A herbicide and plant technology, applied in the field of development of herbicide-resistant grass species, can solve problems such as difficult commercialization of plants

Inactive Publication Date: 2011-06-01
UNIV OF GEORGIA RES FOUND INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, plants produced through genetic engineering methods may be difficult to commercialize due to government regulations and restrictions on the use of genetically modified plants

Method used

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  • Development of herbicide-resistant grass species
  • Development of herbicide-resistant grass species
  • Development of herbicide-resistant grass species

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0140] Callus production obtained from intercalated meristems of plants

[0141] An exemplary explant selection is illustrated in FIG. 18 . Explant tissue can be obtained from shoots containing the uppermost three leaves. Shoots are cut below the lowest leaf nodes, and the tops of individual leaves can be trimmed to maintain spacing during the sterilization procedure. Sections were placed in a bleach solution (20% v / v) for approximately 10 minutes, followed by 70% ethanol for 10 minutes before rinsing with sterile water. The outer (more mature) two leaves are removed, leaving the newest leaves on the stem. New leaves were sterilized in 20% bleach for 1 minute, 70% ethanol for 1 minute, and then rinsed in sterile water. The base of the blade near the nodes is the intervening meristem. The lower 5 mm of this section was removed and seeded on callus induction medium containing MS basal salts (Murashige and Skoog. 1962. Physiol Plant 15:473-497, which is incorporated herein b...

example 2

[0145] Production of callus tissue obtained from immature paspalum flower clusters

[0146] Immature flower clusters were obtained from greenhouse grown plants prior to inflorescence emergence and used as a source of explant tissue for callus generation. Two spikes of flower clusters were separated and surface sterilized with 10% (v / v) bleach containing a few drops of Tween 80 (Tween 80) for 10 minutes, rinsed with sterile water, and then planted on MS medium. The medium contained B5 vitamins (Murashige and Skoog. 1962. Id.; Gamborg et al. 1968. Id.) and 2 mg / L 2-4,D. Explant tissues of 10 genotypes were obtained, including 8 experimental cell lines from the University of Georgia Seashore Paspalum Breeding Program, one harvested ecotype (Mauna Kea ( Mauna Kea) (PI 647892)) and the commercially grown variety "Seaspray". The four explants were planted on each culture plate and covered with Nescofilm TM ) (Karlan Research Products Co; Cottonwood, AZ) sealed culture plates. Th...

example 3

[0150] Dose-response curves of Paspalum to herbicides

[0151]The dose-response of the Paspalum tissue in culture to the sethoxydim application amount was determined using the callus tissue produced from the cultivar "Nanghua" as a model cultivar. By placing the callus from "Sea Spray" on MS / B5 medium containing 2 mg / L 2-4, D and 1 of 8 concentrations of sethoxydim (Murashige and Skog) (Skoog). 1962. supra; Gamborg et al., 1968. supra) to determine the effect of sethoxydim concentration on callus growth. The herbicide application rate was determined 6 times and included concentrations of 0 μM, 2.5 μM, 5 μM, 7.5 μM, 10 μM, 25 μM, 50 μM and 100 μM sethoxydim. Sethoxydim was diluted in methanol and added after the autoclaved medium had cooled to approximately 55°C (to prevent loss of activity due to thermal degradation). Protect the medium from photodegradation by wrapping the container in aluminum foil prior to storage.

[0152] To measure callus growth, 0.5 grams of callus t...

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Abstract

The invention relates to a selected and cultured ACCase inhibitor herbicide-resistant plant -resistant plant from the group Panicodae, or tissue, seed, or progeny thereof, and methods of selecting the same. The invention also relates to methods for controlling weeds in the vicinity of an ACCase inhibitor herbicide-resistant plant.

Description

technical field [0001] The invention disclosed herein generally relates to grasses and methods of developing such grasses that are resistant to selective herbicides. Background technique [0002] Seashore paspalum / Paspalum vaginatum is a warm-season turfgrass generally adapted to sand dune environments. Favorable properties of sea paspalum include its tolerance to salt, flooding and drought. These characteristics make paspalum an excellent turfgrass candidate for playing fields where any or all of these environmental issues can be a problem. For example, golf course designers recommend sea paspalum for new courses in tropical or subtropical coastal areas where salt or water quality can affect turfgrass growth and maintenance. Additionally, many existing golf courses have replaced bermudagrass (Cynodon dactylon) with Paspalum. Compared to Bermudagrass, Paspalum requires less nitrogen and is more tolerant to flushing with saline or poor quality water, which reduces manageme...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/04A01H5/00A01H6/46
CPCA01H5/00A01H1/04A01H6/46A01H1/045A01H1/123
Inventor 保罗·L·雷默道格拉斯·赫卡特韦恩·艾伦·帕罗特
Owner UNIV OF GEORGIA RES FOUND INC
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