Artificial culture method and culture medium for fruiting bodies of cordyceps militaris
A technology of artificial cultivation and culture medium, applied in the field of biomedicine, can solve the problems of large workload, low food utilization efficiency, large energy consumption, etc., and achieve the effects of low cost, large gap and good air permeability
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[0034] Example 1: (1) Preparation of strains:
[0035] Low temperature preservation Cordyceps militaris strain: Cordyceps militaris strain cfcc5973, from China Forestry Microbial Culture Collection Management Center. Cordyceps militaris can also adopt other common cultivated varieties.
[0036] Cordyceps militaris liquid strain: Cultivate according to the following process steps:
[0037] Cultivation of slant bacteria in test tube:
[0038] The weight components of the slant medium are: glucose 2.0g, peptone 1.0g, magnesium sulfate 0.5g, potassium dihydrogen phosphate 0.1g, agar powder 2.0g, water 95g, pH 6-8; sterilize the medium and pack it Put it into the test tube, place it on a slope at room temperature, insert the Cordyceps militaris strain into the slope of the test tube under aseptic conditions, and cultivate in the dark at 20-25℃ for 5-10 days;
[0039] Triangular flask liquid seed culture:
[0040] The weight components of the liquid culture medium are: 1.0g glucose, 1.0g pep...
Example Embodiment
[0052] Example 2: (1) Strain preparation:
[0053] Low temperature preservation Cordyceps militaris strain: Cordyceps militaris strain cfcc5973, from China Forestry Microbial Culture Collection Management Center. Cordyceps militaris can also adopt other common cultivated varieties.
[0054] Cordyceps militaris liquid strain: Cultivate according to the following process steps:
[0055] Cultivation of slant bacteria in test tube:
[0056] The weight components of the slant medium are: glucose 3.0g, peptone 0.5g, magnesium sulfate 0.1g, potassium dihydrogen phosphate 0.5g, agar powder 1.0g, water 105g, pH 6-8; sterilize the medium and pack it Put it into the test tube, place it on a slope at room temperature, insert the Cordyceps militaris strain into the slope of the test tube under aseptic conditions, and cultivate in the dark at 20-25℃ for 5-10 days;
[0057] Triangular flask liquid seed culture:
[0058] The weight components of liquid culture medium are: glucose 3.0g, peptone 0.5g, m...
Example Embodiment
[0070] Example 3: (1) Strain preparation:
[0071] Low temperature preservation Cordyceps militaris strain: Cordyceps militaris strain cfcc5973, from China Forestry Microbial Culture Collection Management Center. Cordyceps militaris can also adopt other common cultivated varieties.
[0072] Cordyceps militaris liquid strain: Cultivate according to the following process steps:
[0073] Cultivation of slant bacteria in test tube:
[0074] The weight components of the slant medium are: 2.5g glucose, 0.7g peptone, 0.3g magnesium sulfate, 0.3g potassium dihydrogen phosphate, 1.7g agar powder, 100g water, pH 6-8; sterilize the medium and pack it Put it into the test tube, place it on a slope at room temperature, insert the Cordyceps militaris strain into the slope of the test tube under aseptic conditions, and cultivate in the dark at 20-25℃ for 5-10 days;
[0075] Triangular flask liquid seed culture:
[0076] The weight components of the liquid medium are: 2g glucose, 0.6g peptone, 0.4g ma...
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