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Artificial culture method and culture medium for fruiting bodies of cordyceps militaris

A technology of artificial cultivation and culture medium, applied in the field of biomedicine, can solve the problems of large workload, low food utilization efficiency, large energy consumption, etc., and achieve the effects of low cost, large gap and good air permeability

Inactive Publication Date: 2011-06-22
LUDONG UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This culture method still has some disadvantages, such as: low grain utilization efficiency, large energy consumption in the sterilization process, and the need for pre-medium configuration, cleaning of residual medium after harvest and washing of culture bottles, etc., the workload Big

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1: (1) bacterial classification preparation:

[0035] Cryopreservation of Cordyceps militaris strains: Cordyceps militaris strains cfcc5973, from China Forestry Microbial Cultures Collection and Management Center. The Cordyceps militaris bacterial classification can also adopt other common cultivars.

[0036] Cordyceps militaris liquid strain: cultured according to the following process steps:

[0037] Test tube slant culture:

[0038] The components of the slant medium in parts by weight are: glucose 2.0g, peptone 1.0g, magnesium sulfate 0.5g, potassium dihydrogen phosphate 0.1g, agar powder 2.0g, water 95g, pH value 6-8; Put it into the test tube, put the inclined surface at room temperature, insert the Cordyceps militaris strain into the inclined surface of the test tube under aseptic conditions, and cultivate in the dark at 20-25°C for 5-10 days;

[0039] Triangular flask liquid seed culture:

[0040] The components in parts by weight of the liquid me...

Embodiment 2

[0052] Embodiment 2: (1) bacterial classification preparation:

[0053] Cryopreservation of Cordyceps militaris strains: Cordyceps militaris strains cfcc5973, from China Forestry Microbial Cultures Collection and Management Center. The Cordyceps militaris bacterial classification can also adopt other common cultivars.

[0054] Cordyceps militaris liquid strain: cultured according to the following process steps:

[0055] Test tube slant culture:

[0056] The components of the slant medium in parts by weight are: glucose 3.0g, peptone 0.5g, magnesium sulfate 0.1g, potassium dihydrogen phosphate 0.5g, agar powder 1.0g, water 105g, pH value 6-8; Put it into the test tube, put the inclined surface at room temperature, insert the Cordyceps militaris strain into the inclined surface of the test tube under aseptic conditions, and cultivate in the dark at 20-25°C for 5-10 days;

[0057] Triangular flask liquid seed culture:

[0058] The components in parts by weight of the liquid med...

Embodiment 3

[0070] Embodiment 3: (1) bacterial classification preparation:

[0071] Cryopreservation of Cordyceps militaris strains: Cordyceps militaris strains cfcc5973, from China Forestry Microbial Cultures Collection and Management Center. The Cordyceps militaris bacterial classification can also adopt other common cultivars.

[0072] Cordyceps militaris liquid strain: cultured according to the following process steps:

[0073] Test tube slant culture:

[0074] The components of the slant medium by weight are: glucose 2.5g, peptone 0.7g, magnesium sulfate 0.3g, potassium dihydrogen phosphate 0.3g, agar powder 1.7g, water 100g, pH value 6-8; Put it into the test tube, put the inclined surface at room temperature, insert the Cordyceps militaris strain into the inclined surface of the test tube under aseptic conditions, and cultivate in the dark at 20-25°C for 5-10 days;

[0075] Triangular flask liquid seed culture:

[0076] The components in parts by weight of the liquid medium are...

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Abstract

The invention discloses an artificial culture method and a culture medium for fruiting bodies of cordyceps militaris. Based on the culture medium in a 500mL culture bottle, the culture medium comprises the following components: 20 to 25g of cottonseed hull and 120 to 140mL of nutrient solution, wherein the nutrient solution comprises the following components in part by weight: 2.0 to 3.0 parts of wheat flour, 1.0 to 1.5 parts of soya flour, 3.0 to 4.0 parts of white sugar, 0.1 to 0.2 part of magnesium sulfate, 0.1 to 0.2 part of potassium dihydrogen phosphate, 0.05 to 0.1 part of zinc sulfate, 0.05 to 0.1 part of sodium chloride and 95 to 105 parts of water, and the pH value of the nutrient solution is 6 to 8. The artificial culture method comprises the following steps of: preparation of the culture medium, sub-packaging, sterilization, cooling, inoculation, culture and harvesting of fruiting bodies. The invention has the advantages of simple operation, high efficiency, energy conservation, short culture period, high yield of fruiting bodies, and no pollution or solid wastes.

Description

Technical field: [0001] The invention relates to a method for artificially cultivating fruit bodies of Cordyceps militaris and a culture medium thereof, belonging to the technical field of biomedicine. Background technique: [0002] Cordyceps militaris, also known as Cordyceps militaris and Cordyceps militaris, belongs to Ascomycotina, Sclerotinia, Spheroides, Ergotaceae, Cordyceps, and is one of the precious Chinese medicinal materials in my country. Cordyceps militaris is a model species of the genus Cordyceps, which belongs to the same species of fungus as Cordyceps sinensis. The chemical components, nutritional components and medicinal functions of the two are very similar; the content of the main medicinal components of Cordyceps militaris, such as cordycepin, is much higher than that of wild Cordyceps sinensis , and it is easier to cultivate artificially than Cordyceps sinensis, so it is selected as the best substitute product of Cordyceps sinensis and a large number o...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 刘林德何洋
Owner LUDONG UNIVERSITY
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