Method for creating novel germ plasm of translocation line through anther culture of intergeneric hybrids between wheat and Leymus multicaulis
A technology of the genus Lyme and the translocation line is applied in the directions of horticultural methods, botanical equipment and methods, plant genetic improvement, etc., to achieve the effects of increasing the cure rate, promoting the mutation frequency and high ear formation rate.
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Embodiment 1
[0039] (1) Lymus multibranches is diploid. After crossing with common wheat, the number of chromosomes in F1 somatic cells is 2n=35, and the variation range of the first generation of backcrossing is 2n=54-57. After two generations of backcrossing, it will be self-crossed again , obtained somatic cell chromosome number 2n=40-49, offspring similar to common wheat, on the basis of multi-generational self-crossing combination resistance identification, selection of materials with exogenous beneficial traits (disease resistance, salt tolerance, etc.), Select lines with 2n=42 and normal meiotic chromosome pairing (two lines each for BC2F3 and BC2F5, one line for BC2F6) as basic materials.
[0040] (2) Anther culture of hybrid offspring of basic material and "bridge variety"
[0041] The above-mentioned basic materials were respectively crossed with the "bridge variety" "Shi 4185" as the female parent, and the anther culture period of the hybrid progeny was selected from the anthers...
Embodiment 2
[0052] It is basically the same as in Example 1, except that the above-mentioned basic materials are respectively crossed with "Bridge Variety" "Jimai 38" as the female parent to obtain anther culture plants of hybrid offspring and obtain new germplasm of translocation lines.
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