Method for creating novel germ plasm of translocation line through anther culture of intergeneric hybrids between wheat and Leymus multicaulis

Abstract

The invention relates to a method for creating a new germ plasm of a translocation line through anther culture of intergeneric hybrids between wheat and Leymus multicaulis. The method comprises the steps as follows: firstly, selecting basis material from filial generation of wheat and Leymus multicaulis; secondly, performing anther culture to filial generation of the basis material and a 'bridge variety'; thirdly, transplanting seedlings of anther culture plants; fourthly, accelerating generation advancement of natural doubling plants in a green house, and doubling haplobiont artificially; and fifthly, reaping pure seeds of the later generations of the anther culture plants, and planting and identifying the pure seeds to obtain the translocation line. In the invention, 'Shi 4185' and 'Jimai 38', which are taken as the female parent, serve as 'bridge variety' to be hybridized with the basis material; and through the optimization of culture medium and culture condition, the highest callus induction of callus reaches 45.4%, the differentiation rate is 92.7%, wherein the differentiation rate of green seedlings is 40%, the final yield of green seedlings is 26.3%, and the survival rate of transplantation is 94%. Under the premise of ensuring that effective selection population is obtained, excellent economical characters of the bridge variety is induced.

Description

technical field [0001] The invention belongs to the field of biotechnology breeding, and in particular relates to a method for cultivating anthers of hybridization between wheat and close wild species to create new germplasm of translocation lines. Background technique [0002] In recent years, due to the increasingly narrow range of genetic variation of wheat varieties, especially the serious shortage of stress resistance resources, there are fewer and fewer available parents, resulting in slow progress in wheat breeding. It is still important to transfer the favorable genes of its close wild species into common wheat for wheat breeding. However, wheat distant hybridization is different from conventional breeding. The close wild species have excellent specific genes (such as salt tolerance, disease resistance genes, etc.) and a large number of redundant genes (such as genes with poor agronomic traits). How to purposefully create a specific translocation line, that is, only...

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Problems solved by technology

However, after clarifying the feasibility of the anther culture approach, how to greatly increase the healing rate and natural doubling rate of hybrid offspring cultured anthers, and ho...

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