Technique for adsorbing and extracting effective part of folium orthosiphoni by employing macroporous resin

An effective part, adsorption and extraction technology, applied to medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., can solve problems such as high toxicity and side effects, damage to the health of operators, and unsuitable for industrial production

Inactive Publication Date: 2011-08-10
HUAQIAO UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction methods of the effective parts of kidney tea mainly include water extraction and alcohol extraction. The separation method includes the preparation step of the upper column liquid of kidney tea, the separation step of the separation column composed of specific separation substances, and the separation process by a specific eluent. The step of eluting the column and the steps of concentrating the eluent, the above-mentioned separation column of the prior art generally adopts a silica gel column, but the silica gel column is used for separation and chromatography is basically a single-component substance, and the eluent Organic solvents (such as benzene, chloroform, etc.) are mostly used, which have relatively large toxic and side effects and damage the health of operators. Therefore, the existing separation and extraction methods are not suitable for industrial production and can be used as a research method for chemical components.

Method used

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  • Technique for adsorbing and extracting effective part of folium orthosiphoni by employing macroporous resin
  • Technique for adsorbing and extracting effective part of folium orthosiphoni by employing macroporous resin
  • Technique for adsorbing and extracting effective part of folium orthosiphoni by employing macroporous resin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0016] Take 50 grams of kidney tea, boil 2-5 times with an aqueous solution of 10-20 times the quality of kidney tea, each time for 60-240 minutes, combine the filtrates obtained each time, concentrate under reduced pressure to a paste, and transfer the concentrate to a In a container of known weight, freeze-dry to obtain the quality of the crude extract; then dissolve the obtained crude extract to prepare a 0.1g / ml solution as the upper column liquid of kidney tea.

[0017] Soak the macroporous resin in 95% medical ethanol overnight, then elute with 95% ethanol solution, collect the eluate and evaporate to dryness until there is no residue, then elute with deionized water until the effluent has no alcohol smell, No cloudiness.

[0018] Put the above-prepared loading solution directly on the column.

[0019] First, the macroporous adsorption resin column is eluted with water until it is colorless, and then eluted with ethanol aqueous solution with a concentration of 20-40%, 4...

Embodiment 2

[0021] Take 50 grams of kidney tea, boil 2-5 times with an aqueous solution of 10-20 times the weight of kidney tea, each time for 60-240 minutes, combine the filtrates obtained each time, concentrate to one-tenth of the volume of the original filtrate, add Equal concentration of 75% ethanol (volume is 1-2 times the volume of kidney tea extract), precipitate protein, filter, filtrate is concentrated under reduced pressure to paste, concentrated solution is transferred to a container of known weight, freeze-dried to obtain The quality of the crude extract; then the crude extract obtained is dissolved and formulated into a solution of 0.1 g / ml as the upper column liquid of Kidney Tea.

[0022] Soak the macroporous resin in 95% medical ethanol overnight, then elute with 95% ethanol solution, collect the eluate and evaporate to dryness until there is no residue, then elute with deionized water until the effluent has no alcohol smell, No cloudiness.

[0023] Put the above-prepared...

Embodiment 3

[0026] Take 50 grams of kidney tea, reflux 2-5 times with 50-95% ethanol aqueous solution of 10-20 times the quality of kidney tea, each time for 60-240 minutes, combine each filtrate, concentrate under reduced pressure to paste , the concentrated solution was transferred to a container of known weight, and freeze-dried to obtain the quality of the extract; then the obtained extract was dissolved and prepared into a 0.1 g / ml solution as the upper column liquid of kidney tea.

[0027] Soak the macroporous resin in 95% medical ethanol overnight, then elute with 95% ethanol solution, collect the eluate and evaporate to dryness until there is no residue, then elute with deionized water until the effluent has no alcohol smell, No cloudiness.

[0028] Put the above-prepared loading solution directly on the column.

[0029] First, the macroporous adsorption resin is eluted with water until it is colorless, and then eluted with ethanol aqueous solution with a concentration of 20-40%,...

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Abstract

The invention discloses a technique for adsorbing and extracting effective parts of folium orthosiphoni by employing macroporous resin. The method comprises the following steps of: 1) extracting the folium orthosiphoni; 2) preparing upper column liquid; 3) separating by a macroporous resin separating column, using ethanol water solution with different polarities as eluant to elute the separating column; and 4) concentrating the eluant. The effective part extracted by the technique is high in content; the content of rosmarinic acid and total flavone reaches above 50 percent, thereby reaching standards of Class 5 of traditional Chinese medicine raw material drugs; and the used eluant has no toxicity and is applicable to industrial production.

Description

technical field [0001] The invention relates to a method for extracting and separating effective parts of kidney tea, in particular to a process method for extracting and separating high-content effective parts of kidney tea by macroporous resin, and belongs to the field of traditional Chinese medicines. Background technique [0002] Kidney tea is a precious medicinal health-care plant and a traditional folk medicine. It is used by folks to treat acute and chronic nephritis, urinary tract infection, urinary tract stones, rheumatoid arthritis and other diseases. Kidney tea also has antibacterial, Anti-inflammatory, increase liver, kidney and spleen blood flow and other functions. Its effective parts are contained rosmarinic acid and flavonoids. At present, the extraction methods of the effective parts of kidney tea mainly include water extraction and alcohol extraction. The separation method includes the preparation step of the upper column liquid of kidney tea, the separati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/53A61P13/00A61P13/04A61P13/12A61P19/02A61P29/00A61P31/04
Inventor 王立强吴振梁锦添罗格莲
Owner HUAQIAO UNIVERSITY
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