Penicilliumaurantiogriseum with function of inducing soybeans to generate atrazine resistance and application
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A technology of atrazine and soybean, which is applied to Penicillium citrinum and its application field, and can solve the problems of high cost of herbicides, easy phytotoxicity of herbicides, and difficulty in weeding
Inactive Publication Date: 2011-08-17
SHENYANG AGRI UNIV
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Problems solved by technology
For soybean fields, weeding is more difficult, and the cost of herbicides in soybean fields is relatively high, and the use of herbicides is prone to phytotoxicity
Method used
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Embodiment 1
[0019] Embodiment 1: the cultivation of Penicillium aurantiogriseum (Penicillium aurantiogriseum) snef960
[0020] Firstly, the fermentation culture of Penicillium aurantiogriseum snef960 strain was carried out, and then the soybean-inducing activity test was carried out on the metabolites after fermentation culture to determine their inducing effect on soybean.
[0021] The thalline of Penicillium aurantiogriseum snef960 is inoculated on the test tube agar medium, and the medium formula is PDA medium, namely composition: 7 potatoes (peeled) 200g, glucose (or sucrose) 10g, agar 17- 20g, add water to 1000mL. Incubate at 25°C for 7 days to obtain test tube species.
[0022] Inoculate the test tube into 250mL Erlenmeyer flask (100mL per bottle) liquid medium again, and the medium formula is: by weight percentage, NaNO 3 0.2%, K 2 HPO 4 0.1%, MgSO 4 .7H 2 O 0.05%, KCl 0.05%, FeSO 4 .7H 2 O 0.001%, sucrose 3%, add distilled water to 1000mL, pH is 7. Cultivate at 25°C, sh...
Embodiment 2
[0023] Embodiment 2: the fermentation of Penicillium aurantiogriseum (Penicillium aurantiogriseum) snef960
[0024] Basically the same as Example 1, the difference is that the liquid-cultured strains were inoculated into a 1000L fermenter for fermentation, cultured at 25°C, pH was 7, and fermented for 7 days.
Embodiment 3
[0025] Embodiment 3: the use of Penicillium aurantiogriseum (Penicillium aurantiogriseum) snef960 fermentation product
[0026] Use the original solution of the fermentation broth to treat the coated soybean seeds at a ratio of 1:50, and after drying, sow the treated soybean seeds normally, at a rate of 350g / 667m 2 After spraying atrazine (atrazine), soybeans can emerge and grow normally.
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Abstract
The invention relates to a method for culturing penicilliumaurantiogriseum snef960 with a function of inducing soybeans to generate an atrazine resistance after soybean seeds are processed and application of the penicilliumaurantiogriseum snef960, and belongs to the technical field of microbial pesticides. A fungus strain snef960 related to the invention is preserved in the China general microbiological culture collection center on Dec 14th in 2010 and the preservation number of the fungus strain snef960 is CGMCC (China General Microbiological Culture Collection Center) No.4468. The fungus strain snef960 is prepared by liquid fermentation culture; and after the soybean seeds are processed by metabolites of the fungus strain snef960, the soybean seeds can generate the atrazine resistance when sprouting. The soybeans processed by the metabolites of the fungus can be interbred with gramineous crops such as corns and the like. A unified atrazine herbicide is adopted to weed. The difficult problem of weeding of interplanting between the corns and the soybeans is solved. The penicilliumaurantiogriseum snef960 has excellent application and development prospect.
Description
Technical field: [0001] The invention belongs to the technical field of microbial pesticides, and specifically relates to a Penicillium citrinum cinerea capable of inducing soybean resistance to atrazine and its application. Background technique: [0002] Atrazine is a dry field herbicide widely used in the world, and its Chinese product name is atrazine. Atrazine (Atrazine), chemical name 2-chloro-4-ethylamino-6-isopropylamino-1,3,5-triazine, is a selective triazine herbicide, introduced in 1952 by Switzerland Ciba- Developed by Geigy Chemical Company. It can be used to control annual grass weeds and broadleaf weeds in corn, sorghum, sugar cane, fruit trees, nurseries, woodlands, etc. Due to its excellent weeding efficacy and low price, it was put into commercial production in 1959. It can be mixed with various amide herbicides such as acetamine, alachlor, and mesopropylmethylamine to enhance the herbicidal activity and application range. Among the dryland herbicides, a...
Claims
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Application Information
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