Method for determining content of hematoxylin in brazilwood by molecular imprinting method

A technology of hematoxylin and hematoxylin, which is applied in the field of molecular imprinting to determine the content of hematoxylin in hematoxylin, can solve the problems of high molecular weight of hematoxylin, achieve the effects of improved sensitivity, strong anti-interference ability, and reduced test error

Active Publication Date: 2011-08-17
江苏泗水纺织有限公司
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Problems solved by technology

At present, molecular imprinting method is mainly used in the detection of small molecules, and hematoxylin

Method used

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  • Method for determining content of hematoxylin in brazilwood by molecular imprinting method

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] The hematoxylin was pulverized into 60 mesh granules, and 10 g of the sample was added to 50 mL of an extract (containing an aqueous solution of 5% ethanol by mass), and extracted by ultrasonic treatment for 5 minutes. The frequency of the ultrasonic generator was 20 kHz and the power was 100 W. The extraction temperature was 20°C. The residue was extracted three more times, and the extracts were 50 mL, 30 mL, and 20 mL, respectively. The extracts were combined, and after filtration, the extract was adjusted to 200 mL (pH=3) with a buffer solution, and then it could be used for electrochemical determination.

[0018] Take hematoxylin standards of different quality, dissolve them in a buffer solution of pH=3, use the hematoxylin-pyrrole / graphite imprinted electrode as the working electrode, the platinum electrode as the counter electrode, and the calomel electrode as the reference electrode. When the speed is 30mV / s, the cyclic voltammetry curve is measured, and the hig...

Embodiment 2

[0020] The hematoxylin was pulverized into 60-mesh granules, and 10 g of the sample was added to 50 mL of an extract (an aqueous solution containing 30% ethanol by mass fraction), and extracted by ultrasonic treatment for 30 minutes. The frequency of the ultrasonic generator was 80 kHz, and the power was 400 W. The extraction temperature was 300C. The residue was extracted three more times, and the extracts were 50 mL, 30 mL, and 20 mL, respectively. The extracts were combined, and after filtration, the extracts were adjusted to 200 mL (pH=9) with a buffer solution, and then they could be used for electrochemical determination.

[0021] Different quality hematoxylin standards were taken and dissolved in a buffer solution of pH=7. The hematoxylin-pyrrole / graphite was used as the working electrode, the platinum electrode as the counter electrode, and the calomel electrode as the reference electrode. At 90mV / s, the cyclic voltammetry curve was measured, and the highest peak posi...

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Abstract

The invention provides a method for determining content of hematoxylin in brazilwood by a molecular imprinting method. The method is characterized in that a hematoxylin-oyrrole/graphite imprinting electrode is used as a three-electrode system in the working electrode; in a buffer solution in which pH is equal to 3 to 11, under the condition that the temperature is 20 to 80 DEG C and the scanning speed is 30 to 600mv/s, the CV curve in the hematoxylin solution of different concentrations can be measured, thus obtaining a linear response relationship, a lower detection limit, and the like. Compared with other electrodes, the method has the advantages that the current intensity is improved by 2 orders of magnitude, and the measurement sensitivity can be greatly improved.

Description

technical field [0001] The invention relates to a method for determining the content of hematoxylin in hematoxylin by molecular imprinting. Background technique [0002] The research on the pharmacological effects of hematoxylin began in the 1970s, and there are many studies at home and abroad. In the past ten years, dozens of reports have shown that hematoxylin has many clinical pharmacological effects, including immunosuppressive effects, antitumor effects, Antibacterial and anti-inflammatory effect, sedative and analgesic effect, hypoglycemic effect, etc. Since hematoxylin is the main component of hematoxylin for its curative effect, the quantitative analysis and research of hematoxylin is of great significance. [0003] Hematoxylin is a plant polyphenol. Because plant polyphenols coexist and are similar to flavonoids, anthraquinones, simple phenols and lignin in plants. It itself is a kind of mixture with very similar structure and properties, and polyphenols are more...

Claims

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Application Information

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IPC IPC(8): G01N27/48G01N27/30
Inventor 高海燕仇小飞侯秀良汪茹刘瑛陈新许芳萍
Owner 江苏泗水纺织有限公司
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