Method for separating and extracting active polypeptide and immune ribonucleic acid by utilizing blood and spleen of animal
A ribonucleic acid and active polypeptide technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., to achieve large economic benefits, fast results, and stress-free effects
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[0027] Below is the method that the present invention utilizes animal blood and spleen to separate and extract active polypeptide and immune ribonucleic acid, and its steps are as follows:
[0028] 1 Fat removal: take the spleen of livestock and poultry, and remove the fat in the raw material;
[0029] 2 Weighing and cleaning: Accurately weigh out the raw materials after removing fat, and wash them with purified water for 2 to 3 times;
[0030] 3 Coarse pulverization: mix the spleen, blood and purified water in corresponding proportions, grind them with a colloid mill for several minutes to make a homogenate; the weight ratio of spleen to purified water is 1:2, and the weight ratio of spleen to blood is 1: 5;
[0031] 4 ultrafine crushing: the homogenate is carried out ultrafine crushing through a cell crusher, and the solution after crushing is packed into a sterilized container;
[0032] 5 Inactivation: Add formaldehyde solution to 0.2% of the total amount of crushing solu...
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