Method for separating and extracting active polypeptide and immune ribonucleic acid by utilizing blood and spleen of animal

A ribonucleic acid and active polypeptide technology, applied in the direction of DNA / RNA fragments, recombinant DNA technology, etc., to achieve large economic benefits, fast results, and stress-free effects

Inactive Publication Date: 2011-08-31
SHANDONG PROVINCE ZI COUNTY JINXIN LIVESTOCK & POULTRY
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AI Technical Summary

Problems solved by technology

With the rapid development of the breeding industry, the diseases of livestock and poultry are also increasing, the dosage of individual drugs is increasing, and the cost of feeding is gradually increasing, resulting in a decline in breeding benefits

Method used

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  • Method for separating and extracting active polypeptide and immune ribonucleic acid by utilizing blood and spleen of animal

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Embodiment Construction

[0027] Below is the method that the present invention utilizes animal blood and spleen to separate and extract active polypeptide and immune ribonucleic acid, and its steps are as follows:

[0028] 1 Fat removal: take the spleen of livestock and poultry, and remove the fat in the raw material;

[0029] 2 Weighing and cleaning: Accurately weigh out the raw materials after removing fat, and wash them with purified water for 2 to 3 times;

[0030] 3 Coarse pulverization: mix the spleen, blood and purified water in corresponding proportions, grind them with a colloid mill for several minutes to make a homogenate; the weight ratio of spleen to purified water is 1:2, and the weight ratio of spleen to blood is 1: 5;

[0031] 4 ultrafine crushing: the homogenate is carried out ultrafine crushing through a cell crusher, and the solution after crushing is packed into a sterilized container;

[0032] 5 Inactivation: Add formaldehyde solution to 0.2% of the total amount of crushing solu...

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Abstract

The invention provides a method for separating and extracting active polypeptide and immune ribonucleic acid by utilizing the blood and spleen of an animal. The method is as follows: the advanced lysis technology is utilized, the blood and spleens of healthy livestock and poultry are used as raw materials, the fat of the raw materials is removed, cells are broken, and inactivation, freezing and thawing, centrifugation, coarse filtration, chromatographic separation, fine filtration, preparing and subpackaging are performed to prepare active polypeptide oral solution. The product has small molecular weight and does not have antigenicity at all; and after being used, the product can not stimulate the organism to generate antibody and can not be neutralized by antibody. By using the active polypeptide oral solution, the animal immunity can be enhanced and the anti-infectious capability of organism can be increased; the oral solution can not be damaged by trypsin, DNA enzyme and RNA enzyme, the oral solution has good application effect; and the oral solution has high activity, low clinical dosage, no toxic or side effect and no medicament residue, is safe to use and takes effect fast. The active polypeptide oral solution can be used to prevent and cure the diseases caused by virus infection.

Description

technical field [0001] The invention relates to a method for separating and extracting active polypeptide and immune ribonucleic acid (iRNA) by using animal blood and spleen. The active polypeptide and immune ribonucleic acid (iRNA) are mainly used for the prevention and treatment of viral diseases in the animal. Background technique [0002] In recent years, with the improvement of people's living standards, the demand for livestock and poultry food has increased year by year, which has led to the unprecedented development of the breeding industry. With the rapid development of the breeding industry, the diseases of livestock and poultry are also increasing, the dosage of individual medicines is increasing, and the cost of feeding is gradually increasing, resulting in a decline in breeding benefits. The food safety problems caused by the hazards of western medicine and product residues have attracted more and more attention from the Chinese people. If this continues, it ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K1/36C07K1/34C07K1/16C12N15/117
Inventor 蒋士策蒋明达虞文洲夏雪
Owner SHANDONG PROVINCE ZI COUNTY JINXIN LIVESTOCK & POULTRY
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