Device for culturing in vitro cells through single axis rotating and online shearing

Abstract

The invention discloses a device for culturing in vitro cells through single axis rotating and online shearing, belonging to the technical field of biomechanics engineering. The device mainly comprises a fixed rotating drum, flow chambers, a peristaltic pump, a liquid storage bottle, a waste liquid bottle, a motor and a base, wherein the flow chambers are fixed on the fixed rotating drum; the motor drives the fixed rotating drum to rotate at 360 degrees to ensure the sum of gravitational vector of a circle of rotation of the fixed rotating drum to be zero to simulate the microgravity effect; the rotational speed is voluntarily set along with rotation of the motor; the liquid storage bottle and the waste liquid bottle are fixed on the base; the liquid storage bottle is connected with the peristaltic pump; the peristaltic pump pumps the culture liquid to the flow chambers while the fixed rotating drum rotates to apply the fluid shear stress to the flow chambers; and the culture liquid flows back to the waste liquid bottle via the flow chambers. The device can be used for studying and exploring the biological effects of the cells after the cells are subjected to mechanical stimulation under the action of the microgravity, provides cellular and molecular mechanism basis for the astronauts to cope with the movement measures during space flight and has application prospect.

Description

technical field [0001] The invention relates to the field of biomechanical engineering research, in particular to a device capable of simulating microgravity effect culture for adherent cells and simultaneously applying controllable shear stress to the cells. Background technique [0002] In recent decades, the exploration and research of spaceflight has attracted more and more attention from various countries and scholars, but how to develop and establish an effective medicine for the space environment based on the theory of cell and molecular biology during a long period of spaceflight? There are still few protective and countermeasures. During long-term or short-term space flight, the cytoskeleton of mechanically sensitive cells is rearranged, and the functions of cell signal transduction, gene expression, cell proliferation and differentiation are abnormal, which in turn leads to various physiological and pathological changes in the astronauts' body, such as bone loss, i...

AI Technical Summary

Problems solved by technology

[0006] The present invention aims at the lack of devices capable of simulating microgravity effects on in vitro cultured cells while applying fluid shear stress loads, and proposes a single-axis rotating online shearing in vitro cell culture device, which realizes two-dimensional simulating microgravity effects At the same time, the function of the flow chamber to load the cells with flow shear stress is also realized, which is used to study the response of cells to mechanical stimuli under microgravity conditions

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