Application of adult stem cells in treating malignant solid tumors

A technology of adult stem cells and solid tumors, which is applied in the field of stem cell therapy and can solve the problems that human adult stem cells have not been reported in the treatment of malignant solid tumors.

Inactive Publication Date: 2011-09-21
CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

[0008] Human adult stem cells have not been rep

Method used

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  • Application of adult stem cells in treating malignant solid tumors
  • Application of adult stem cells in treating malignant solid tumors
  • Application of adult stem cells in treating malignant solid tumors

Examples

Experimental program
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Embodiment Construction

[0062] (1) Fusion technology of human adult stem cells and tumor cells:

[0063] 1) Cell fusion technology (Robinson, 1979).

[0064] A. 0.125% trypsin digested cells EC9706 cells and hMSCs cells, prepared into a single cell suspension,

[0065] B. Counting, hMSCs:EC9706=1:10 mixed in a conical centrifuge tube, washed with PBS, 1000rpm, 10 minutes, three times;

[0066] C. Discard the supernatant, resuspend the cells with H-DMEM, 1000rpm, 10 minutes;

[0067] D. After carefully absorbing the supernatant, flick, rotate and shake to make the cells fully disperse into a paste, and place it in a 37°C water bath;

[0068] E. Add 1ml of 50% sterile PEG1500 pre-warmed at 37°C while shaking, and drop it within 1 minute

[0069] a) 37°C water bath for 90 seconds, during which time the centrifuge tube was gently shaken;

[0070] b) Gently add 10ml of 37°C pre-warmed H-DMEM 10ml dropwise to stop the effect of PEG, and drop it within 3 minutes, try not to stir up the cells when droppi...

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Abstract

The invention relates to application of adult stem cells in treating malignant solid tumors, in particular to application of adult stem cells in preparing a cell preparation for treating malignant solid tumors. The inventor fuses human mesenchymal stem cells and human hemopoietic stem cells respectively with human esophageal carcinoma cells, and injects the fusion cells into SCID (severe combined immune deficiency)/mice and nude mice, and as a result, the emerging rate of tumors of the fusion cells is obviously reduced and the volume thereof is also reduced. The stem cell preparation provided by the invention can obviously inhibit the growth of malignant solid tumors.

Description

technical field [0001] The present invention relates to the field of stem cell therapy. Specifically, the present invention relates to the use of human adult stem cells in the treatment of malignant solid tumors. More specifically, the present invention relates to the use of human adult stem cells, especially human mesenchymal stem cells and human hematopoietic stem cells in the preparation of cell preparations for treating malignant solid tumors. Background technique [0002] In the 21st century society is progressing and developing, but human beings are still suffering from many diseases. Tissue and organ defects and dysfunction caused by major diseases, such as cardiovascular and cerebrovascular diseases, malignant tumors, trauma, aging and genetic defects, are difficult medical problems for human beings to overcome. Stem cell regenerative medicine and stem cell therapy medicine are new medical methods, which provide a new way for the treatment of major diseases, prolon...

Claims

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Application Information

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IPC IPC(8): A61K35/12A61K35/28A61K35/14A61P35/00
Inventor 陆士新
Owner CANCER INST & HOSPITAL CHINESE ACADEMY OF MEDICAL SCI
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