Processing method for activity stability of enzyme in human serum
A processing method and technology of human serum, applied in the preparation of test samples, etc., can solve the problems of instability, inconsistent measurement results, lack of comparability, etc., to solve the problem of interoperability and meet the needs of clinical use.
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[0030] Step 1. Collect the fresh human serum on the day of the experiment and place it in a closed freeze-thaw tube, freeze it below -60°C until about 1000ml is collected.
[0031] Step 2. Take the serum out of the freezer and place it naturally at room temperature for 4 hours until it is completely dissolved by visual observation, and then freeze it below -60°C for more than 2 hours; then place the serum at 4°C for 10 hours until it is completely dissolved by naked eye observation , and then freeze at -60°C for 2 hours; then rinse the freeze-thaw tube containing the serum with water at 10°C until it is completely dissolved by visual inspection.
[0032] Step 3: Use low-high speed ∮ 15-30cm qualitative filter paper to filter out fibrin filaments, various antigen-antibody complexes and protein complex particles in serum.
[0033] Step 4. The human serum after primary filtration is aseptically filtered under conventional aseptic conditions, using a microporous filter membrane wi...
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