Processing method for activity stability of enzyme in human serum

A processing method and technology of human serum, applied in the preparation of test samples, etc., can solve the problems of instability, inconsistent measurement results, lack of comparability, etc., to solve the problem of interoperability and meet the needs of clinical use.

Active Publication Date: 2012-11-21
北京航天总医院
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AI Technical Summary

Problems solved by technology

The characteristic of this type of calibrator is that the calibrator (enzyme activity) has a long stable period, but usually has obvious matrix effects, which often leads to large variations and lack of comparability in the measurement results of different analysis systems. Most of them are not traceable, that is, they are not consistent with the measurement results of internationally recognized reference methods; the other is the calibrator based on human serum, the biggest problem of this type of calibrator is instability
At present, there are no research results on the stability of enzyme activity in human serum based on the use of human serum with the same matrix as the measurement sample to effectively solve the interoperability problem at home and abroad

Method used

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  • Processing method for activity stability of enzyme in human serum

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Experimental program
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Embodiment

[0030] Step 1. Collect the fresh human serum on the day of the experiment and place it in a closed freeze-thaw tube, freeze it below -60°C until about 1000ml is collected.

[0031] Step 2. Take the serum out of the freezer and place it naturally at room temperature for 4 hours until it is completely dissolved by visual observation, and then freeze it below -60°C for more than 2 hours; then place the serum at 4°C for 10 hours until it is completely dissolved by naked eye observation , and then freeze at -60°C for 2 hours; then rinse the freeze-thaw tube containing the serum with water at 10°C until it is completely dissolved by visual inspection.

[0032] Step 3: Use low-high speed ∮ 15-30cm qualitative filter paper to filter out fibrin filaments, various antigen-antibody complexes and protein complex particles in serum.

[0033] Step 4. The human serum after primary filtration is aseptically filtered under conventional aseptic conditions, using a microporous filter membrane wi...

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Abstract

The invention provides a processing method for activity stability of enzyme in human serum, comprising the following steps: step 1: collecting serum; step 2: removing unstable ingredients; step 3: removing impurities such as fibrins and the like; step 4: carrying out aseptic treatment; step 5: conducting split charging and storing; and step 6: inspecting aseptic state. In the invention, the humanserum having the same substrate with the sample to be measured is used for effectively solving the problem of interoperability; the collected human serium is processed by a series of technologies so that the enzyme activity in the human serum can keep stable within a period, thus meeting the clinic requirement; and the activity of the enzyme in the human serum prepared by the processing method can be kept stable for more than one year.

Description

technical field [0001] The invention relates to the field of medical value traceability, in particular to a method for processing human serum. Background technique [0002] Quantitative traceability is a hot issue in the field of laboratory medicine in recent years. Its purpose is to solve the problem of accurate and comparable measurement of clinical routine methods. The core issue is that the calibrators of clinical routine methods should have traceability and good interoperability with the measurement samples. After 2005, China has gradually established a reference system, and the traceability of calibrators has been basically solved from a technical point of view. The interoperability problem is still a difficult problem that plagues the accuracy and comparability of clinical routine methods. Due to the high complexity and instability of human serum samples, finding and establishing calibrators that have good interoperability with human serum and meet the stability requi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N1/28G01N1/42G01N1/34
Inventor 陈宝荣邵燕孙慧颖胡滨陈琦李玲李月玲
Owner 北京航天总医院
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