Method and purpose for constructing traditional Chinese medicine (TCM) spleen deficiency syndrome AIDS monkey model

A technology for AIDS and spleen deficiency, which can be used in pharmaceutical formulations, medical preparations containing active ingredients, and preparation of samples for testing. It can solve problems such as difficulties in screening traditional Chinese medicine, achieve repeatability and stability, and promote theoretical research Effect

Inactive Publication Date: 2011-11-02
GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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  • Abstract
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Problems solved by technology

[0003] However, animal models suitable for screening anti-AIDS by traditional Chinese medicine have not

Method used

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  • Method and purpose for constructing traditional Chinese medicine (TCM) spleen deficiency syndrome AIDS monkey model
  • Method and purpose for constructing traditional Chinese medicine (TCM) spleen deficiency syndrome AIDS monkey model

Examples

Experimental program
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Embodiment 1

[0020] Example 1 The first method and application of constructing the AIDS monkey model of TCM spleen deficiency syndrome

[0021]

[0022] 1. Experimental animals: Select healthy adult male rhesus monkeys, SIV, simian retrovirus type D (SRV) and simian T lymphocytic virus type I (STLV-I) antibodies are negative through serological testing, and there are no symptoms and signs of spleen deficiency and qi deficiency ;

[0023] 2 Modeling drug: rhubarb gavage solution

[0024] 2.1 Preparation process: Take the raw rhubarb medicinal material, crush it into a coarse powder, weigh 5 kg, add 4 times the amount of water, put it in a stainless steel bucket and stir evenly, soak for 0.5 h, then put the bucket in a boiling water bath, continue heating and keeping warm Stir for 20 min, filter with gauze, add 8000 mL of boiling water to the residue, stir for 20 min, and filter with four layers of gauze. Concentrate the medicinal liquid filtered for the second time to a small volume u...

Embodiment 2

[0084] Example 2 The Second Method and Application of Constructing the TCM Spleen Deficiency Syndrome AIDS Monkey Model

[0085]

[0086] 1. Experimental animals: Select healthy adult male rhesus monkeys, which are negative for SIV, simian retrovirus (SRV), simian B virus and simian T lymphocytic virus type I (STLV-I) antibodies through serological testing, without spleen deficiency , Qi deficiency symptoms and signs;

[0087] 2 Modeling drug: rhubarb gavage solution

[0088] 2.1 Preparation process: Take the raw rhubarb medicinal material, crush it into a coarse powder, weigh 5 kg, add 4 times the amount of water, put it in a stainless steel bucket and stir evenly, soak for 0.5 h, then put the bucket in a boiling water bath, continue heating and keeping warm Stir for 20 min, filter with gauze, add 8000 mL of boiling water to the residue, stir for 20 min, and filter with four layers of gauze. Concentrate the medicinal liquid filtered for the second time to a small volume un...

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Abstract

The invention discloses a method and purpose for constructing a TCM spleen deficiency syndrome AIDS monkey model. Among existing AIDS experimental animals, a monkey resembles to mankind the most. The inventor successfully constructs an HIV infected rhesus monkey TCM spleen deficiency syndrome composite model by two methods respectively. The first method is to carry out gavage with raw rhubarb concentrate to a create spleen deficiency syndrome and then carry out intravenous injection of infected SIV viruses. The second method is to carry out gavage with raw rhubarb concentrate to create the spleen deficiency syndrome after rhesus monkeys is infected with SIV viruses through intravenous injection. Based on the understanding of infection course, pathogenic processes and pathological changes of AIDS viruses in a spleen deficiency syndrome model, research on attack of infected target organs, target tissue and target cells to the immune system, on response of cells of the immune system, on the evolution law of a TCM syndrome, etc., is carried out. The established TCM spleen deficiency syndrome HIV infected monkey composite model in the invention plays an irreplaceable role in studying TCM pathogenesis and evolution of symptomatology of TCM for AIDS and in evaluating drug effects of anti-AIDS TCMs and compounds.

Description

technical field [0001] The invention relates to a method and application of constructing a TCM model, in particular to a method and application of constructing a TCM spleen deficiency syndrome AIDS model. Background technique [0002] At present, the treatment of AIDS mainly includes the application of highly active antiretroviral therapy (HARRT), immunotherapy, biological therapy, vaccines, and traditional Chinese medicine treatment. Among them, the clinical treatment of traditional Chinese medicine has the characteristics of syndrome differentiation and treatment. Individualized treatment can be adopted according to the patient's own physical characteristics and symptom manifestations. Traditional Chinese medicine can regulate the body's immune system to prolong the survival time of patients with poison, with less side effects and good compliance. Traditional Chinese medicine plays a positive therapeutic role especially in the asymptomatic period after HIV infection. In ...

Claims

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Application Information

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IPC IPC(8): A61K49/00A61K35/76A61K36/708G01N33/571G01N21/00G01N21/64G01N1/28G01N1/31A01K67/027
Inventor 符林春李茂清胡英杰何金洋张苗苗陈芝喜刘政胡董仁陈津岩董伯振周红燕李志强刘抗伦
Owner GUANGZHOU UNIVERSITY OF CHINESE MEDICINE
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