Method for liquid-liquid extraction separation of tryptophan by using hydrophobic amide functionalized ionic liquid

A technology of amide functionalization and ionic liquid, applied in the direction of organic chemistry, can solve the problems of no more than 50% and unsatisfactory extraction effect, and achieve the effect of simple operating conditions, good extraction effect and green separation method

Inactive Publication Date: 2011-11-02
DALIAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In recent years, people have used conventional ionic liquids to conduct preliminary research on the separation of amino acids, but the extraction effect is not ideal, and the general extraction rate does not exceed 50%. The functionalization of ionic liquids can greatly improve the extraction performance of ionic liquids. However, Its separation research on tryptophan has not been reported yet

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  • Method for liquid-liquid extraction separation of tryptophan by using hydrophobic amide functionalized ionic liquid

Examples

Experimental program
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Effect test

Embodiment 1

[0020] 2.0ml [EIMCH 2 CONBu][NTf 2 ] The ionic liquid was added to 1ml of 0.0500mol / l tryptophan solution, stirred for 20min, and allowed to stand for stratification. After ultraviolet detection, the concentration of tryptophan in the aqueous phase was reduced to 0.0058mol / l, and the extraction rate was 88.3%.

Embodiment 2

[0022] 1.0ml [EIMCH 2 CONBu][NTf 2 ] The ionic liquid was added to 1ml of 0.0500mol / l tryptophan solution, stirred for 20min, allowed to stand for stratification, and detected by ultraviolet light, the concentration of tryptophan in the aqueous phase was reduced to 0.0081mol / l, and the extraction rate was 83.7%.

Embodiment 3

[0024] 0.5ml [EIMCH 2 CONBu][NTf 2 ] The ionic liquid was added to 1ml of 0.0500mol / l tryptophan solution, stirred for 20min, allowed to stand for stratification, and detected by ultraviolet light, the concentration of tryptophan in the aqueous phase was reduced to 0.0112mol / l, and the extraction rate was 77.6%.

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Abstract

The invention relates to a method for liquid-liquid extraction separation of tryptophan by using hydrophobic amide functionalized ionic liquid, which comprises the following steps of: adding an amide functionalized ionic liquid extracting agent and a tryptophan aqueous solution into a processing container under the normal pressure and temperature according to a proportion, stirring and reacting for 20-30min in an acidic condition, wherein the stirring speed is 330 rpm; standing still for layering after reaction is ended; and separating out a water layer and an extracting agent layer. The water layer is an extracted tryptophan aqueous solution; and the detection shows that the content of the amino acid is reduced by 69% to 88%. The method disclosed by the invention is free of pollution to environment and is an efficient and environment-friendly tryptophan separation method.

Description

technical field [0001] The invention belongs to the field of solvent extraction, and relates to an extraction and separation method of a tryptophan aqueous solution, in particular to a liquid-liquid extraction and separation method for a hydrophobic amide functionalized ionic liquid for tryptophan. Background technique [0002] Tryptophan is one of the essential amino acids in human and animal life activities. It plays an important role in the growth, development and metabolism of humans and animals. It is widely used in medicine, food and feed, etc. [0003] The separation methods of tryptophan mainly include precipitation method, ion exchange method, adsorption method and extraction method. However, these methods have many disadvantages, such as the difficulty in recovering the precipitating agent of the precipitation method, and the serious pollution of waste liquid discharge; the operation of the ion exchange method is complicated; the selectivity of the adsorption metho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07D209/20
Inventor 李长平李琢尹静梅李华汐
Owner DALIAN UNIV
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