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Antrodia camphorata cyclohexenone compound for inhibiting skin cancer tumor cell growth

A tumor cell and compound technology, applied in the field of Antrodia cinnamomea cyclohexenone compound used to inhibit the growth of skin cancer tumor cells, can solve unpublished specific problems and the like

Inactive Publication Date: 2011-11-09
GOLDEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Although it can be known from many current experiments that the extract of Antrodia Cinnamomea has the aforementioned effects, and its components have been analyzed one after another, but no specific research has been published on which active ingredients in the extract can contribute to the anticancer effect of Antrodia Cinnamomea. The relevant active ingredients need to be clarified by further experimental research. Therefore, if the ingredients contained in the extract can be found to effectively inhibit tumor growth, it will be beneficial to the research on the mechanism of Antrodia Cinnamomea inhibiting cancer, and the application of Antrodia Cinnamomea to cancer such as skin Cancer treatment and prevention are of great help

Method used

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  • Antrodia camphorata cyclohexenone compound for inhibiting skin cancer tumor cell growth
  • Antrodia camphorata cyclohexenone compound for inhibiting skin cancer tumor cell growth
  • Antrodia camphorata cyclohexenone compound for inhibiting skin cancer tumor cell growth

Examples

Experimental program
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Effect test

Embodiment 1

[0022] 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone separate

[0023] About 100 grams of Antrodia mycelium, fruiting bodies or the mixture of the two are placed in a conical flask, and an appropriate proportion of water and alcohols (70% to 100% alcohol aqueous solution) is added, wherein the alcohols are relatively Preferably it is ethanol, stir and extract at 20-25°C for at least 1 hour, then filter with filter paper and 0.45μm filter membrane, collect the filtrate to obtain the extract of Antrodia camphorata.

[0024] The previously collected Antrodia camphorata extract was analyzed using a high performance liquid chromatography (High Performance Liquid chromatography) with an RP18 chromatographic tube (column), and methanol (A) and 0.1% to 0.5% acetic acid aqueous solution (B ) as the mobile phase (the solution ratio is: 0-10 minutes, B ratio is 95%-20%; 10-20 minutes, B ratio is 20%-10%; 20-35 minutes, B ratio 10% to 90%; 35 t...

Embodiment 2

[0027] Activity test against skin cancer tumor cells in vitro

[0028] In order to further test the inhibitory effect of the compound found in Example 1 on tumor cells, this example will first take the isolated compound in Example 1 according to the anti-tumor drug screening mode of the National Cancer Institute (NCI) in the United States. 4-Hydroxy-2,3-dimethoxy-6-methyl-5(3,7,11-trimethyl-2,6,10-dodecatriene)-2-cyclohexenone compound , added to the culture medium containing human skin cancer tumor cells A431 to test the viability of tumor cells. Among them, the test of cell viability can be analyzed by conventional MTT analysis method, and the skin cancer tumor cell A431 is a human skin cancer cell line (human epidermoid carcinoma cell line).

[0029] MTT assay is a commonly used analytical method for analyzing cell proliferation, percent of viable cells, and cytotoxicity. Among them, MTT (3-[4,5-dimethylthiazol-2-yl]2,5-diphenyltetrazolium bromide) is a yellow dye, which ...

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Abstract

The invention relates to a new application of a compound. 4-hydroxy-2,3-dimethoxy-6-methy-5(3,7,11-trimethyl-dodeca-2,6,10-trienyl)-cyclohex-2-enone is separated and purified from antrodia camphorata extract. The cyclohexenone compound can be applied to inhibition of skin cancer tumor cell growth, and also can be applied to medicinal compositions for inhibiting skin cancer tumor cell growth.

Description

technical field [0001] The present invention relates to a new application of a compound, in particular to a use of a compound isolated and purified from antrodia cinnamomea (Antrodia cinnamomea) extract to inhibit the growth of skin cancer tumor cells. Background technique [0002] With the increase of outdoor leisure activities and the destruction of the atmospheric ozone layer, the incidence and death rate of skin cancer in the world are gradually increasing, causing major problems in human health. There are racial differences in the occurrence of skin cancer. The attack rate is the most common in Caucasians, followed by yellow people, and the lowest in black people. And the age group where skin cancer is prone to occur is more in adults. Most skin cancers occur on the face, neck, ears, forearms, and backs of hands. Causes include long-term, heavy exposure to sunlight, chronic chemical exposure, or prolonged exposure to radiation exposure etc. [0003] At present, the cl...

Claims

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Application Information

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IPC IPC(8): A61K31/122A61P35/00
Inventor 刘胜勇温武哲郭茂田
Owner GOLDEN BIOTECH
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