Gelatine-PCL (posterior cruciate ligament) dental hemostatic sponge and preparation method thereof

Abstract

The invention provides gelatine-PCL (posterior cruciate ligament) dental hemostatic sponge and a preparation method thereof. The preparation method comprises the following steps of: firstly, stirring a gelatine solution with the mass fraction of 5-20% with a PCL solution with the mass fraction of 10-20% at the temperature of 25-60 DEG C to react for 1-5 hours so as to prepare a combined solution, then adding auxiliary additives to the combined solution, continuously stirring for 1-3 hours at the temperature of 25-60 DEG C to prepare a mixed solution, pouring the mixed solution into a mould, freezing for 0.5-4 hours at the temperature of minus 20 DEG C, and finally freeze drying and moulding in a freeze drying machine to obtain the gelatine-PCL hemostatic sponge. The gelatine-PCL hemostatic sponge prepared by the invention has good retention property, hemostatic property and anti-inflammatory bacteriostatic effect; the product is safe and effective without toxic or side effect; waste can be degraded without causing environmental pollution; and the gelatine-PCL hemostatic sponge can be widely applied as a postoperation hemostatic material for dental extraction and other oral surgeries and has a good market application prospect.

Description

technical field

[0001] The invention relates to the field of preparation of medical materials, in particular to a gelatin-PCL dental hemostatic sponge with good mechanical properties, retention, degradability, better hemostasis, pain relief, anti-inflammatory and antibacterial effects and its preparation method. Background technique

[0002] Tooth extraction is one of the common operations in the department of stomatology, and bleeding after tooth extraction is a common complication after tooth extraction. Tooth extraction bleeding causes systemic factors or local factors, and the bleeding at the present invention is caused by local factors, such as tearing of gingival mucosa, residual granulation tissue in the alveolar socket, rupture of alveolar tubules and corruption and shedding of blood clots in tooth extraction wounds; Inflammation caused by inflammation and periapical periodontitis makes the wound surface bleed for a long time after tooth extraction; the rough operat...

Examples

Embodiment 1

[0028] The gelatin trifluoroethanol solution with a mass fraction of 20% and the PCL trifluoroethanol solution with a mass fraction of 10% were blended according to the ratio of gelatin to PCL dry weight of 1:1, and stirred at 50°C for 5 hours to obtain gelatin and PCL. compound fluid. Add auxiliary additives to the composite liquid of gelatin and PCL according to the ratio of auxiliary additives (blended according to the ratio of tea polyphenols and thrombin dry weight of 1:1) to the compound liquid with a weight ratio of 1:250, and stir at 50°C About 1 hour, it is configured into a mixture of gelatin, PCL and auxiliary additives. The mixture of gelatin, PCL and auxiliary additives was poured into the mold, frozen at -20°C for 2 hours, placed in a lyophilizer, and kept at about -70°C for about 10 hours. Then the mold is taken out to obtain the gelatin-PCL dental hemostatic sponge.

Embodiment 2

[0030] The gelatin hexafluoroisopropanol solution with a mass fraction of 10% and the PCL hexafluoroisopropanol solution with a mass fraction of 10% were blended according to the ratio of gelatin to PCL dry weight of 1:5, and stirred at 40 ° C. React for 4 hours to obtain a composite solution of gelatin and PCL. Add gelatin and PCL according to the proportion of auxiliary additives (tea polyphenol, chitosan, thrombin, white and dry weight ratio of 2:2:1:1) and compound liquid weight ratio of 1:500 Add auxiliary additives to the composite solution, stir at 40°C for about 2 hours, and prepare a mixture of gelatin, PCL and auxiliary additives. The mixture of gelatin, PCL and auxiliary additives was poured into the mold, frozen at -20°C for 4 hours, placed in a lyophilizer, and kept at about -70°C for about 10 hours. Then the mold is taken out to obtain the gelatin-PCL dental hemostatic sponge.

Embodiment 3

[0032] The gelatin trifluoroethanol solution with a mass fraction of 5% and the PCL trifluoroethanol solution with a mass fraction of 20% were blended according to the ratio of gelatin to PCL dry weight ratio of 1:10, and stirred and reacted at 40°C for 2 hours to obtain gelatin and PCL compound solution. According to the proportion of auxiliary additives (the ratio of dry weight of tea polyphenol, benzylphenol and thrombin blended at 1:1:1) to the compound liquid at a weight ratio of 1:100, add auxiliary additives to the compound liquid of gelatin and PCL Additives, stirred at 40°C for about 2 hours, configured as a mixture of gelatin, PCL and auxiliary additives. The mixture of gelatin, PCL and auxiliary additives was poured into the mold, frozen at -20°C for 2 hours, placed in a lyophilizer, and kept at about -50°C for about 15 hours. Then the mold is taken out to obtain the gelatin-PCL dental hemostatic sponge.

technical field

[0001] The invention relates to the field of preparation of medical materials, in particular to a gelatin-PCL dental hemostatic sponge with good mechanical properties, retention, degradability, better hemostasis, pain relief, anti-inflammatory and antibacterial effects and its preparation method. Background technique

[0002] Tooth extraction is one of the common operations in the department of stomatology, and bleeding after tooth extraction is a common complication after tooth extraction. Tooth extraction bleeding causes systemic factors or local factors, and the bleeding at the present invention is caused by local factors, such as tearing of gingival mucosa, residual granulation tissue in the alveolar socket, rupture of alveolar tubules and corruption and shedding of blood clots in tooth extraction wounds; Inflammation caused by inflammation and periapical periodontitis makes the wound surface bleed for a long time after tooth extraction; the rough operat...

Examples

Embodiment 1

[0028] The gelatin trifluoroethanol solution with a mass fraction of 20% and the PCL trifluoroethanol solution with a mass fraction of 10% were blended according to the ratio of gelatin to PCL dry weight of 1:1, and stirred at 50°C for 5 hours to obtain gelatin and PCL. compound fluid. Add auxiliary additives to the composite liquid of gelatin and PCL according to the ratio of auxiliary additives (blended according to the ratio of tea polyphenols and thrombin dry weight of 1:1) to the compound liquid with a weight ratio of 1:250, and stir at 50°C About 1 hour, it is configured into a mixture of gelatin, PCL and auxiliary additives. The mixture of gelatin, PCL and auxiliary additives was poured into the mold, frozen at -20°C for 2 hours, placed in a lyophilizer, and kept at about -70°C for about 10 hours. Then the mold is taken out to obtain the gelatin-PCL dental hemostatic sponge.

Embodiment 2

[0030] The gelatin hexafluoroisopropanol solution with a mass fraction of 10% and the PCL hexafluoroisopropanol solution with a mass fraction of 10% were blended according to the ratio of gelatin to PCL dry weight of 1:5, and stirred at 40 ° C. React for 4 hours to obtain a composite solution of gelatin and PCL. Add gelatin and PCL according to the proportion of auxiliary additives (tea polyphenol, chitosan, thrombin, white and dry weight ratio of 2:2:1:1) and compound liquid weight ratio of 1:500 Add auxiliary additives to the composite solution, stir at 40°C for about 2 hours, and prepare a mixture of gelatin, PCL and auxiliary additives. The mixture of gelatin, PCL and auxiliary additives was poured into the mold, frozen at -20°C for 4 hours, placed in a lyophilizer, and kept at about -70°C for about 10 hours. Then the mold is taken out to obtain the gelatin-PCL dental hemostatic sponge.

Embodiment 3

[0032] The gelatin trifluoroethanol solution with a mass fraction of 5% and the PCL trifluoroethanol solution with a mass fraction of 20% were blended according to the ratio of gelatin to PCL dry weight ratio of 1:10, and stirred and reacted at 40°C for 2 hours to obtain gelatin and PCL compound solution. According to the proportion of auxiliary additives (the ratio of dry weight of tea polyphenol, benzylphenol and thrombin blended at 1:1:1) to the compound liquid at a weight ratio of 1:100, add auxiliary additives to the compound liquid of gelatin and PCL Additives, stirred at 40°C for about 2 hours, configured as a mixture of gelatin, PCL and auxiliary additives. The mixture of gelatin, PCL and auxiliary additives was poured into the mold, frozen at -20°C for 2 hours, placed in a lyophilizer, and kept at about -50°C for about 15 hours. Then the mold is taken out to obtain the gelatin-PCL dental hemostatic sponge.