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Antonospora locustae strain and application thereof in prevention and control of locust

A technology for locust microsporidia and locusts, which is applied in the directions of methods for using spores, applications, and methods based on microorganisms, can solve problems such as weak pathogenicity of locust microsporidia, achieves broad application prospects, and improves the level of locust control technology. Effect

Active Publication Date: 2013-06-05
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the pathogenicity of Nos. locusts is generally weak, so it is necessary to improve the virulence of Nos. locusts

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Embodiment 1, the acquisition of Microsporidia locust AL2008-01 strain

[0024] The diseased East Asian migratory locusts collected in the field were ground, filtered, examined under a microscope, and then purified by centrifugation, and different strains of wild locust Nosporum were isolated from the insect bodies. Apply various strains of wild microsporidia locusts on corn leaves at a series of concentrations, and then feed them to the 4th instar gnats of migratory locusts for 2 days, harvest the earlier dead insects, and purify them by grinding, filtering, centrifuging, etc. locust microsporidia. After 30 generations of continuous screening, the AL2008L-04 strain of Antonospora locustae was obtained.

[0025] It can be seen under the optical microscope (400 times) that the spores of the AL2008L-04 strain are oval, and the size is (6.13±1.37) μm×(3.17±0.53) μm, which is larger than that of the existing strain (5.40±0.50) μm×( 3.11±0.37) μm is slightly larger.

[00...

Embodiment 2

[0027] Pathogenicity identification of embodiment 2, AL2008-01 strain

[0028] The corn leaves in the embodiments can be replaced by wheat leaves.

[0029] One, the preparation of the spore of AL2008-01 strain

[0030] 1. Hatch the eggs of Locusta migratoria manilensis at 28-30° C., feed fresh corn leaves every day at 28-32° C. and 60-70% relative humidity, and raise them until the 4th instar locust nymph.

[0031] 2. The spore liquid of AL2008L-04 strain (1×10 6 spores / ml) are smeared on fresh corn leaves, and the mass ratio of corn leaves and spore liquid is 1: 1, which is used to feed the 4th instar locust gnat, and is continuously fed for 2 days, and is then normally fed with fresh corn leaves until the locusts die; Conditions: the temperature is kept at 25-32° C., the relative humidity is kept at about 60%, the light time is 16 hours / day (the light source is an incandescent lamp), and the corn leaves are fed twice a day.

[0032] 3. Grind the dead locust body and filte...

Embodiment 3

[0043] Embodiment 3, the preparation of preparation and its application

[0044] 1. Preparation of preparations with AL2008-01 strain

[0045] The spores of the AL2008L-04 strain prepared by step 1 of Example 2, Tween-80, sucrose and water were used to prepare a soluble suspension, and the concentration of the spores of the AL2008L-04 strain was 10 7 Spores / ml, the mass percent content of Tween-80 is 4%, and the mass percent content of sucrose is 3%.

[0046] Second, the application of preparations

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Abstract

The invention discloses an Antonospora locustae strain and application thereof in prevention and control of locust. The Antonospora locustae disclosed by the invention is named as AL2008L-04 strain, is obtained by isolating and screening from the field diseased Asiatic migrotory locust, and was collected in China General Microbiological Culture Collection Centre (CGMCC) in No. 3 of Beichen West Road No. 1 Court, Chaoyang District, Beijing on May 23, 2011 with the culture collection number of CGMCC No. 4901. Antonospora locustae AL2008L-04 strain CGMCC No. 4901 is abbreviated as AL2008L-04 strain. The locust prevention and control agent disclosed by the invention can be used for preventing and controlling grassland locust, migratory locust and other locusts. The product belongs to a biological pesticide, has no toxic or harmful effects on environment, human, livestock and poultry, is safe for natural enemy, and has broad application prospects. The Antonospora locustae strain provided by the invention has significance in improving the level of a prevention and control technique on locust in China.

Description

technical field [0001] The invention relates to a locust microsporidia strain and its application in controlling locusts. Background technique [0002] Locust disasters, floods and droughts often occur alternately, and have always been the three natural disasters that seriously threaten my country's agricultural production and affect people's lives. There are more than 600 species of locusts recorded in my country, of which more than 60 species can cause harm to agriculture, forestry, and animal husbandry. [0003] The current method of controlling locusts is mainly to use chemical pesticides to prevent and control. Although chemical control can quickly reduce the population density of locusts, it often causes adverse consequences such as polluting the environment, killing a large number of beneficial animals, poisoning humans and animals, and developing insecticide resistance. [0004] Biopesticide locust control can not only effectively control locust infestation, but als...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/10C12N3/00A01N63/00A01P7/04C12R1/90
Inventor 张龙
Owner CHINA AGRI UNIV
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