Brucella Omp16 protein antigen epitope polypeptides and application thereof

Abstract

The invention discloses Brucella Omp16 protein antigen epitope polypeptides and application thereof. The polypeptides are formed by sequences shown as SEQ ID No.1 or / and SEQ ID No.4 or / and SEQ ID No.3 or / and SEQ ID No.2 or / and SEQ ID No.5 included in the amino acid sequences of the polypeptides or the sequences formed through substitution and / or deletion of one or / and more amino acid residues of the sequences and can also be polypeptides derived by the shown sequences and having a Brucella immunogenicity function. The polypeptides can form recombinant vectors or expression cassettes or transgenic cells or recombinant bacteria. The polypeptides can be applied to preparation of regents or medicines for diagnosing or preventing or treating diseases caused by Brucella. The screened polypeptides are chemically synthesized, can release high-concentration IL-4 after DC stimulation and can be used as objective antigens for detecting the serum infected with the Brucella or antigens for preparing Omp16 protein monoclonal antibodies.

Description

technical field [0001] The invention relates to a polypeptide, specifically a Brucella Omp16 protein antigen epitope polypeptide and application thereof. Background technique [0002] Brucella, a Gram-negative facultative intracellular parasite, is a serious zoonotic disease that widely infects domestic animals, wild animals and humans. Brucella in domestic animal animals mainly infects sheep, cattle and pigs Among them, the main ones that are pathogenic to humans are Brucella malta and Brucella abortus; Brucellosis infecting domestic animals causes epididymitis in male animals and abortion, placental inflammation and infertility in pregnant animals; for humans, Brucella Bacteroidosis causes acute inflammation and many symptoms similar to influenza infection, including wave fever, sweating, back pain, and weakness. In some patients, acute clinical symptoms can last for more than a year and eventually lead to chronic persistent infection. Clinical symptoms include irregular ...

AI Technical Summary

Problems solved by technology

[0003] At present, the effective vaccines used to prevent brucellosis all over the world are attenuated live vaccines. The use of various vaccines not only interferes with the differential diagnosis of vaccine immunity and natural infection, but also all vaccine strains are harmful to humans to varying degrees. Virus-causing, even some vaccines are highly pathogenic to humans, and the research effect of safe and reliable inactivated vaccines is poor, and they cannot play an immune protection role

The study of genetically engineered vaccines has been concerned and worked hard by researchers all over the world in recent decades. However, due to the diversity and complex structure of Brucella's immune antigens, vaccine antigens that can be used in field trials have not been found. It is an effective way to solve this problem by developing a subunit vaccine with high preventive protection.

[0004] Most of the expression tools in the prior art can only express one protein, while Brucella has a variety of immune-related proteins. Studies have shown that the expression of a single protein cannot form an effective protective vaccine antigen, and multiple prevention and control vaccines can be prepared by expression The cost of the subunit vaccine is high, and the composition is complex; while the component that determines the function of the immune protein is a tiny epitope located on the protein. The mining and identification of the immune epitope of the immune-related protein can effectively extract the brucellosis Effective immune antigen components in bacteria, and also provide direct guidance for protein structure and function research, the determination of epitope antigen components can provide good antigen components for detection, prevention or treatment

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