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SNP (Single Nucleotide Polymorphism) related to chick carcass trait and application thereof

A technology for carcass traits and populations, applied in recombinant DNA technology, DNA/RNA fragments, etc., to achieve the effect of saving time, rapid and accurate auxiliary screening, and high accuracy

Inactive Publication Date: 2013-03-06
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, it is speculated that the mutation site of its family gene may also have a significant genetic effect on the fat traits of chickens, and there are few studies on the polymorphism of the SREBF2 gene, and there are almost no studies on animals.

Method used

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  • SNP (Single Nucleotide Polymorphism) related to chick carcass trait and application thereof
  • SNP (Single Nucleotide Polymorphism) related to chick carcass trait and application thereof
  • SNP (Single Nucleotide Polymorphism) related to chick carcass trait and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Acquisition of SREBF2 Gene Polymorphic Sites

[0022] Chicken wing vein blood collection, all blood samples were 1mL / bird, anticoagulated with EDTA, and the blood samples were frozen at -20°C. Adopt conventional phenol-chloroform method to extract genomic DNA from above-mentioned chicken blood, detect with 1.0% agarose electrophoresis, can see that DNA band is clear, bright, without impurity band, fully meets the requirement of follow-up PCR experiment ( figure 1 )

[0023] The chicken SREBF2 gene mRNA sequence (Gene ID: XM_4162222) predicted in the Erlang Mountain mountain chicken SREBF2 gene cDNA sequence obtained in this test and GenBank, and the SREBF2 gene DNA sequence (Gene ID: NC_006088.2) in GenBank, carry out primer design, There are 17 pairs in total. See Table 1 for primer details.

[0024] Table 1 Details of primers used for SNP scanning of SREBF2 gene

[0025]

[0026]

[0027] Use the above 17 pairs of primers to perform PCR amplificat...

Embodiment 2

[0030] Example 2 Genotype Frequency and Allele Frequency of SREBF2 Gene Polymorphic Site

[0031] 2.1 Genotype frequency and gene frequency

[0032] (1) Genotype frequency: refers to the ratio between various genotypes of a certain trait in a population. Because the detection result of PCR-SSCP method is a co-dominant allele. Therefore, the phenotype frequency is also the genotype frequency.

[0033] Genotype frequency = number of genotypes / total number of detection groups

[0034] (2) Allele frequency: denoted as Pi, which represents the ratio of the number of a certain allele in a population to the total number of all alleles occupying the same gene locus, and the value is between 0 and 1.

[0035] Pi=[2(ii)+(ij1)+(ij2)+...(ijn)] / 2N

[0036] Pi: Frequency of the i-th allele

[0037] i: Homozygous multiple alleles

[0038] j1, j2, ... jn: the 1st to nth alleles that co-occur with i

[0039] (3) Gene locus χ 2 Independence test: SAS software package (SAS Institute Inc....

Embodiment 3

[0047] Example 3 Polymorphic information content (PIC) of each site of SREBF2 gene

[0048] PIC is used to estimate the polymorphism of the marker gene, and it is an index indicating the degree of DNA variation. PIC>0.5 is high polymorphism; 0.25<PIC<0.5 is moderate polymorphism; PIC<0.25 is low polymorphism.

[0049] PIC = 1 - ( Σ i = 1 n P i 2 ) - ( Σ i = 1 n - 1 Σ j = i + 1 n ...

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Abstract

The invention relates to the field of molecular biology detection, in particular to SNP (Single Nucleotide Polymorphism) related to chick carcass trait and application thereof. The invention provides 10 SNP markers of a chick SREBF2 gene and a method for detecting the carcass trait of a chick colony by applying the SNP markers. The invention further provides a primer for detecting the SNP markersand a kit comprising the same. The SNP markers provided by the invention can be applied to molecular marker assistant breeding of chicken, can assist in screening quickly and accurately, and have theadvantages of early screening, saving in time, low cost and high accuracy.

Description

technical field [0001] The invention relates to the field of molecular biology detection, in particular to SNPs related to chicken carcass traits and applications thereof. Background technique [0002] With the improvement of living standards, people have higher and higher requirements for meat quality, and fat traits are important indicators for quality evaluation of high-quality chicken. Local breed chicken is famous for its excellent meat quality, delicious taste and rich nutrition, attracting more and more consumers. After years of scientific selection and breeding of high-quality broilers, the production performance of high-quality broilers has been significantly improved. However, while the performance of chickens increased, the meat quality decreased (Rance K. et al., 2002). Subcutaneous fat and abdominal fat deposits in broilers increased, while intramuscular fat content decreased. The steps of conventional methods for measuring these fat traits are cumbersome and...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/11
Inventor 邱莫寒朱庆孙建胡耀东王彦刘益平兰丹
Owner SICHUAN AGRI UNIV
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