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Preparation method of royal jelly polypeptide

A technology for royal jelly and queen bee larvae, applied in the field of biomedicine, can solve the problems of few polypeptides, difficult to screen biologically active polypeptides, unsuitable for royal jelly proteolysis, etc.

Inactive Publication Date: 2013-06-19
BEIJING NORMAL UNIVERSITY +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, so far, there have been few studies on the enzymatic hydrolysis of royal jelly polypeptides, and most of the enzymatic hydrolysis methods are carried out by exogenous enzymes such as pepsin, trypsin, papain, etc., which are not suitable for the hydrolysis of royal jelly protein, and it is difficult to screen to obtain the right peptides. Bioactive peptides that are truly effective for the human body

Method used

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  • Preparation method of royal jelly polypeptide
  • Preparation method of royal jelly polypeptide
  • Preparation method of royal jelly polypeptide

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preparation example Construction

[0053] The invention discloses a method for preparing a royal jelly polypeptide, and those skilled in the art can learn from the content of this article and appropriately improve the process parameters to realize it. In particular, it should be pointed out that all similar replacements and modifications are obvious to those skilled in the art, and they are all considered to be included in the present invention. The method and application of the present invention have been described through preferred embodiments, and the relevant personnel can obviously make changes or appropriate changes and combinations to the method and application described herein without departing from the content, spirit and scope of the present invention to realize and Apply the technology of the present invention.

[0054] All reagents in the royal jelly polypeptide provided by the invention can be purchased from the market. Both royal jelly and queen bee larvae were purchased from professional beekeep...

Embodiment 1

[0057]Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. After adding 50mmol / L phosphate buffer with a pH value of 7 and diluting with the mass volume ratio (g / mL) of the intestinal tract of the worm body (g / mL) at 1:1, grind it into a homogenate with an electric homogenizer under ice-bath conditions. Centrifuge at 20,000×g for 20 min at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of suspended solution, the lower layer of sedimentation, and the middle layer is recovered. The middle layer was centrifuged again at 20000×g at 4°C for 20 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.

[0058] Take part of the intestinal enzyme solution, and use the BCA method to d...

Embodiment 2

[0063] Fresh queen bee larvae aged 2 to 3 days were taken, washed with isotonic ice-cold 0.9% saline, filtered, dissected one by one and collected all intestinal tracts of the worms. After adding 50mmol / L phosphate buffer solution with a pH value of 7 and a 1:1 mass volume ratio (g / mL) to the intestinal tract of the parasite, it was ground to a homogenate with an electric homogenizer under ice-bath conditions. Centrifuge at 18000×g for 30 min at 4°C. After centrifugation, the suspension is divided into three layers, namely, the upper layer of floating matter (lipid components), the middle layer of suspended solution, the lower layer of sedimentation, and the middle layer is recovered. The middle layer was centrifuged again at 18000×g at 4°C for 30 min, and the middle layer was taken to obtain a yellow transparent intestinal enzyme solution, which was stored in a -20°C refrigerator.

[0064] Take part of the intestinal enzyme solution, and use the BCA method to detect the prote...

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Abstract

The invention relates to the technical field of biomedicines, and in particular relates to a preparation method of a royal jelly polypeptide. 1-3kDa of the royal jelly polypeptide is obtained by extracting intestinal enzymes from the bodies of queen bee larva and simulating the enzymolysis environment in the queen bee bodies to carry out enzymolysis on royal jelly. The prepared royal jelly polypeptide can be used for preparing Abeta inhibitors.

Description

technical field [0001] The invention relates to the technical field of biomedicine, in particular to a preparation method of royal jelly polypeptide. Background technique [0002] In recent years, with the increase of the average life expectancy of human beings, the proportion of the elderly population has been increasing, and the aging of my country's population is becoming more and more obvious. Alzheimer's disease (AD), as the most common type of senile dementia, has caused the whole society to extensive attention. Alzheimer's disease is a complex multifactorial chronic progressive mental decline disease characterized by memory loss and cognitive dysfunction, clinical manifestations are progressive memory and cognitive decline, language barriers, psychomotor abnormalities and other symptoms . In the early stage of the disease, the near memory is mainly decreased, and the distant memory is also affected in the later stage of the disease, and the daily life is affected. ,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06
Inventor 冯成强孙平颜慧李虎臣
Owner BEIJING NORMAL UNIVERSITY