Method for identifying specificity of Biluochun tea from Tungting Mountains, Soochow
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A specific and Biluo technology, which is applied in the application field of chemical fingerprint identification in the identification of local specialties, can solve the problems of non-specific distinction of safety indicators, inability to analyze and evaluate tea quality, and achieve excellent application effect significant effect
Inactive Publication Date: 2011-11-23
SUZHOU TESTAR TESTING TECH
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However, the national standard GB/T 18957-2008F Geographical Indication Product--Dongting (Mountain) Biluochun Tea cannot specifically distinguish Dongting (Mountain) Biluochun Tea from other real estate "Biluochun Tea" by sensory indicators, phy...
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Embodiment 1
[0028] Take about 2 grams of tea samples into a 50ml Erlenmeyer flask, add 20ml of water to ultrasonic extraction for 60 minutes, control the temperature at 60 degrees, and obtain the test sample liquid after precision filtration.
[0029] Liquid chromatography conditions: use ODS chromatographic column, elute with 50% acetonitrile aqueous solution as mobile phase, ultraviolet detector, detection wavelength 260nm, injection volume 20μl, record the peak area and retention time of the spectrum.
[0030] Comparing the spectrum of the tea sample with the spectrum of the Dongting (Mountain) Biluochun standard sample using fingerprint calculation software, the similarity between the two is 85%.
[0031] Another tea sample about 3 grams was taken to a 100ml Erlenmeyer flask with a stopper, and 30ml of ether was added to reflux for extraction for 60 minutes, and the test sample liquid was obtained after precision filtration.
[0032] Gas chromatography conditions: use DB-5 capillary c...
Embodiment 2
[0036] Take about 5 grams of tea samples into a 100ml Erlenmeyer flask, add 20ml of water for ultrasonic extraction for 90 minutes, control the temperature at 80 degrees, and obtain the test sample liquid after precision filtration.
[0037] Liquid chromatography conditions: use a C8 chromatographic column, elute with a mobile phase of 50% aqueous methanol, a UV detector, a detection wavelength of 254 nm, and an injection volume of 10 μl. Record the peak area and retention time of the spectrum.
[0038] Comparing the spectrum of the tea sample with the spectrum of Dongting (Mountain) Biluochun physical standard sample using fingerprint calculation software, the similarity between the two is 90%.
[0039] Take about 5 grams of tea leaves to a 100ml Erlenmeyer flask with a stopper, add 50ml of diethyl ether for reflux extraction for 60 minutes, and obtain a test sample solution after precision filtration.
[0040]Gas chromatographic conditions: use DB-1 capillary chromatographic...
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Abstract
The invention discloses a method for identifying the specificity of Biluochun tea from Tungting Mountains, Soochow. The method disclosed by the invention comprises the following steps: using tea which is prepared from native populations in a protective range meeting the national standard GB/T 18957-2008F geographical indication tea provision as a standard substance sample and extracting the fingerprint of the tea as a comparison reference group; respectively extracting a specificity liquid chromatogram fingerprint and a specificity gas chromatogram fingerprint of a tea sample to be identified and comparing by utilizing fingerprint computing software; based on the identification reference that the similarity between the liquid chromatogram fingerprint and the standard substance sample fingerprint and the similarity between the gas chromatogram fingerprint and the standard substance sample fingerprint are both higher than 80%, qualitatively and quantitatively identifying the genuineness and quality of the tea sample. By the implementation of the method, the practical restriction that the fake Biluochun tea from Tungting Mountains are hardly identified by the traditional identification method based on perception indexes, rationalization indexes, safety indexes and the like can be broken through, and the genuineness of the Biluochun tea from Tungting Mountains can be effectively and accurately identified, therefore the invention provides a solid guarantee for protecting geographical indication tea.
Description
technical field [0001] The invention relates to an identification method of local special products, in particular to an application technology of chemical fingerprint identification in the identification of local special products. Background technique [0002] Dongting (Mountain) Biluochun tea (that is, "Suzhou Dongting Mountain real estate Biluochun tea" in the title) is one of the most important famous teas in my country. The buds and leaves of Dongting (Mountain) Biluochun tea are tender, slender in shape, curled into snails, and the velvet is exposed. say. This is determined by its unique ecological environment and harvesting process. [0003] In addition to Dongting (mountain) Biluochun tea in Suzhou, "Biluochun" green tea is also produced in many places across the country. Due to the outstanding advantages of Dongting (Mountain) Biluochun tea in terms of brand and quality, there are a large number of other "Biluochun" imitations of Dongting (Mountain) Biluochun tea ...
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