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Porcine parvovirus recombinant pseudorabies virus genetic engineering live vector vaccine and preparation method thereof

A technology of porcine pseudorabies virus and live vector vaccines, applied in genetic engineering, botany equipment and methods, biochemical equipment and methods, etc., to achieve good preventive effects, promote social and economic progress, and promote development

Inactive Publication Date: 2011-12-07
河南亚卫动物药业有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Traditional routine vaccines have played a certain role in the prevention and control of these two diseases, but various vaccines have defects and deficiencies in varying degrees, such as the potential threat of recombination and virulence reversion of attenuated vaccines, and the potential threat of inactivated vaccines. Unstable immunity, etc.

Method used

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  • Porcine parvovirus recombinant pseudorabies virus genetic engineering live vector vaccine and preparation method thereof
  • Porcine parvovirus recombinant pseudorabies virus genetic engineering live vector vaccine and preparation method thereof
  • Porcine parvovirus recombinant pseudorabies virus genetic engineering live vector vaccine and preparation method thereof

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Embodiment 1

[0024] A preparation method of porcine parvovirus recombinant pseudorabies virus genetically engineered live vector vaccine, which comprises the steps of:

[0025] ① Using the genome of porcine parvovirus NADL-2 strain as a template, PCR amplifies the porcine parvovirus VP2 gene, specifically:

[0026] According to the genome sequence of porcine parvovirus NADL-2 strain reported by AnaI.Ranz et al., a pair of PCR primers including its structural protein VP2 gene were designed. location. The primers were synthesized by Bao Biological Engineering (Dalian) Co., Ltd. The primer sequences are as follows:

[0027] P1: 5′-TT GGA TCC ATG GCA ACC TCA CCA CC-3′ (the underline is the BamHI restriction site)

[0028] P2: 5′-TACA GGT ACC GTA AAC ACA TGA GAG CTTG-3′ (the underline is the KpnⅠ restriction site)

[0029] Use P1 and P2 as primers, and use the porcine parvovirus NADL-2 strain virus gene as a template for PCR amplification. The PCR reaction uses a 50 μL reaction system...

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Abstract

The invention relates to a porcine parvovirus recombinant pseudorabies virus genetically engineered live vector vaccine, the main material of which is composed of porcine parvovirus VP2 gene, porcine pseudorabies virus vector and VERO cells; the vaccine is composed of porcine parvovirus VP2 gene and porcine pseudorabies virus The rabies virus vector is recombined and then obtained by virus screening. The specific method is as follows: first, using the porcine parvovirus NADL-2 strain genome as a template, PCR amplifies the porcine parvovirus VP2 gene; then clones the VP2 gene into the porcine pseudorabies virus vector PCK to obtain the PCK-VP2 plasmid; - VP2 plasmid and pseudorabies virus PRV genome were co-transfected into VERO cells; finally, porcine parvovirus-porcine pseudorabies recombinant virus PCK-VP2-PRV was obtained through screening. Experiments have proved that it has good immune protection in piglets.

Description

Technical field [0001] The present invention is a biotechnology field, which involves a genetic engineering vaccine, especially a pseudo -rabbit virus genetic carrier vaccine and its preparation method for a pseudo -rabies gene engineering carrier. Background technique [0002] The rapid development of the aquaculture industry has benefited from the progress of science and technology to the greatest extent, but it also brings hidden dangers.The advancement of science and technology has led the breeding industry to intensive and factorized development. The susceptibility of livestock and poultry to pathogenic microorganisms has increased, diseased frequently occurs, cross -infections, and severe mixed infections have caused great economic losses in production. [0003] In recent years, with the development of the pig industry, the successive emergence of large -scale intensive pig farms has also followed, and disease problems have also followed. Various livestock and poultry disea...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K39/295A61K39/23A61K39/245A61P31/20A61P31/22C12N15/869
Inventor 李明魁张遂平吴宁鹏李奇杨慧敏郭芳茹邵艳华王菊萍
Owner 河南亚卫动物药业有限公司
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