Preparation method of alligator alternanthera effective fraction extract

A technology of effective parts of A. japonicus, applied in the field of preparation of extracts of effective parts of A. japonicus, capable of solving the problems of few antiviral drugs, uncontrollable quality, backward technology, etc., achieving high content of effective substances and reducing potential safety hazards , The effect of simple process

Active Publication Date: 2013-06-19
武汉回盛生物科技股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method is backward in technology, uncontrollable in quality, and very easy to cause lead poisoning, so this preparation process will not be included in the future pharmacopoeia
At present, there are no reports on the extraction and purification methods of the effective substances of A. japonicus. However, there are few effective antiviral drugs at home and abroad, and antiviral drugs are still a hot spot for domestic and foreign scholars.

Method used

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  • Preparation method of alligator alternanthera effective fraction extract

Examples

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Embodiment 1

[0022] A preparation method for the extract of effective fractions of A. japonicus, the steps are as follows:

[0023] a. Weigh the medicinal material of lotus seed herb, add 6-10 times of its weight in ethanol with a concentration of 60v / v%, soak for 1 hour, then reflux extraction, extract three times in total, and combine the three extracts to obtain the ethanol extract;

[0024] The addition of 60v / v% ethanol is 10 times of the weight of the medicinal materials during the first extraction, 8 times of the weight of the medicinal materials for the second time, and 6 times of the weight of the medicinal materials for the third time;

[0025] The time of the first reflux extraction was 2 hours, and the time of the second and third reflux extraction was 1 hour.

[0026] b. The ethanol extract is decompressed to recover ethanol, and the remaining medicinal solution is diluted to a concentration of 0.15 g / ml in terms of crude drug amount, and filtered after standing to obtain a fi...

Embodiment 2

[0031] Example 2 Pilot scale-up test

[0032] A preparation method for the extract of effective fractions of A. japonicus, the steps are as follows:

[0033] a. Weigh 12Kg of Herba Hollow Lotus Medica, add 6-10 times the weight of the medicinal materials in 60v / v% ethanol for soaking for 1 hour, then heat and reflux for extraction, extract three times in total, and combine the three extracts to obtain the ethanol extract;

[0034] The addition of 60v / v% ethanol is 10 times of the weight of the medicinal materials during the first extraction, 8 times of the weight of the medicinal materials for the second time, and 6 times of the weight of the medicinal materials for the third time;

[0035] The time for the first reflux extraction is 2 hours, and the time for the second and third reflux extraction is 1 hour;

[0036] b. The ethanol extract is decompressed to recover ethanol, the remaining medicinal solution is diluted to a volume of 80L (concentration is 0.15g / ml in terms of ...

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Abstract

The invention discloses a preparation method of an alligator alternanthera effective fraction extract, which comprises the following steps: 1. extracting alligator alternanthera with alcohol under reflux; 2. recycling the alcohol extract under reduced pressure, diluting the medicinal liquor, and filtering; 3. adsorbing the filtrate through D101 macroporous adsorbent resin columns, and eluting with distilled water and alcohols with different concentrations; and 4. recycling the eluent under reduced pressure, concentrating to a relative density of 1.01-1.10, and drying to obtain the alligator alternanthera effective fraction extract. The general flavone content of the alligator alternanthera effective fraction extract product is up to 3-5 wt%, the transfer rate is 50-70%; and the general saponin content is up to 15-18 wt%, and the transfer rate is 40-60%. No related data about the preparation technique of the effective fraction extract has been reported at home and abroad. The inventionhas the advantages of simple extraction and purification technique and higher content of effective substances, lowers the production cost, saves the energy, reduces the potential safety hazard in theproduction, and satisfies the modern requirements for traditional Chinese medicine industry.

Description

technical field [0001] The invention belongs to the technical field of extracting and purifying effective fractions of traditional Chinese medicines, and more specifically relates to a preparation method of an extract of effective fractions of A. japonicus. The method can effectively extract total flavonoids and total saponins in A. japonicus. Background technique [0002] Alternanthera philoxeroides (Mart.) Griseb is a perennial herbaceous plant of Amaranthaceae (Amaranthaceae) Alternanthera. effect. It has been reported in the literature in the past 20 years that Asteroides japonicus has anti-virus, immune-regulating, and anti-liver damage effects. It is clinically used to treat viral diseases such as urticaria, influenza, Japanese encephalitis, and epidemic hemorrhagic fever. Nutella plants contain a variety of chemical components, and the chemical components that have been isolated and identified so far include: triterpenoids, flavonoids, alkaloids, lactams, organic aci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/21A61P31/12
Inventor 张卫元刘焱文叶晓川陈树和李森张周菊刘国庆
Owner 武汉回盛生物科技股份有限公司
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