Method for transforming stem tips of plants and special tool thereof
A plant and shoot tip technology, which is applied in the field of plant shoot tip transformation methods and special tools, can solve the problems of difficult batch genetic transformation, low callus induction rate and plant regeneration rate, and achieves overcoming genotype dependence and body regeneration. The effect of cell mutation, improving the transformation efficiency of shoot apex, and simple operation
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[0058] Example 1. Using wheat stem tip transformation system to obtain transgenic wheat
[0059] 1. The establishment of wheat shoot tip transformation system
[0060] 1. Material
[0061] A. Wheat varieties:
[0062] Zhongmai 12 wheat (hereinafter referred to as wild-type wheat), winter nature; strong tillering ability, high ear formation rate; ear fusiform, long awn, white husk, white kernel, kernel horny; yield factors in ear number and ear per mu The number of grains is more, the thousand-grain weight is low; the plants are shorter, the plant height is about 65 cm in the water-saving regional test, and the high-fertilizer regional test is about 70 cm, the lodging resistance is good; the cold resistance is good; the resistant, slow stripe rust, slow leaf Rust, susceptible to powdery mildew and stem rust.
[0063] Purchased from the Seed Sales Department of Beijing Zhongnong Golden Land Agricultural Development Research Center.
[0064] B. Transfection of recombinant bacteria: C58 / pC...
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[0161] Example 2. Obtaining transgenic Salicornia salicina using the transformation system of Salicornia salicina
[0162] 1. Experimental group
[0163] 1. Material
[0164] A. S. euroopeae L. seeds (hereinafter referred to as wild-type S. salina) were purchased from Dafeng City, Jiangsu Province, Jinglong Marine Industry Development Co., Ltd.
[0165] B. Transfection of recombinant bacteria: C58 / pCAMBIA3300-ubinos
[0166] Recombinant Agrobacterium C58 / pCAMBIA3300-ubinos is a recombinant bacteria obtained by transferring the named plasmid pCAMBIA3300-ubinos into Agrobacterium C58.
[0167] The plasmid pCAMBIA3300-ubinos contains the ubiquitin promoter and the selection marker gene encoding phosphinothricin acetyltransferase (PAT, phosphinothricin acetyltransferase) gene bar.
[0168] The Agrobacterium strain C58 was disclosed in the document "Overexpression of Organellar and Cytosolic AtHSP90 in Arabidopsis thaliana Impairs Plant Tolerance to Oxidative Stress. Hongmiao Song, Pengxiang F...
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