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Lamb placenta polypeptide and preparation method thereof

A technology of sheep placenta and sheep placenta, which is applied in the preparation method of peptides, chemical instruments and methods, peptides, etc., can solve the problems of losing the meaning of using placenta, the content of peptides and amino acids, etc., and achieve the improvement of infertility, Improve skin condition, advanced technology effect

Inactive Publication Date: 2012-01-25
HUZHOU KANGHAISI BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, we believe that the above two preparations have their own disadvantages. The former destroys the natural active substances in the placenta during the hydrolysis process and loses the meaning of using the placenta; although the latter has a variety of natural active substances, the content of polypeptides and amino acids is very small, such as It can integrate the two, not only retain the natural active substances in the placenta, but also increase the content of peptides and amino acids

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] A preparation method of sheep placenta polypeptide, the method comprises the following steps:

[0033] 1) Pretreatment of sheep embryos and sheep placenta raw materials: take sheep embryos and sheep placenta aged 2-3 months as raw materials, wash and remove impurities, and crush them into coarse powder at a low temperature of 0-5°C.

[0034] 2) Superfine pulverization of sheep embryos and sheep placenta, cell wall breaking: freeze the coarse powder of sheep embryos and sheep placenta at low temperature (-40℃~-45℃) for 2 hours, then ultrafine pulverization at low temperature (-20℃) (WZJ100 medicinal super micro pulverizer), the cell wall is broken to reach a particle size of 0.1-10μm.

[0035] 3) Extraction and enzymatic hydrolysis of sheep embryo and sheep placenta: add 6 times of distilled water to extract the sheep embryo and sheep placenta serum after ultrafine grinding and cell wall breaking, then filter to obtain filtrate A and precipitate A, and precipitate A with...

Embodiment 2

[0049] A method for preparing sheep placenta polypeptide, the method except that papain is used for enzymatic hydrolysis in step 3), other technical solutions are as described in Example 1.

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Abstract

The invention relates to a preparation method of lamb embryo and lamb placenta biological products. A lamb placenta polypeptide preparation method comprises the following steps of: 1) lamb embryo and lamb placenta raw material pretreatment; 2) lamb embryo and lamb placenta super micro pulverization and cell wall breaking; 3) lamb embryo and lamb placenta extraction and zymohydrolysis; and 4) grading refining as follows on lamb embryo and lamb placenta stock solution obtained in the third step according to the molecular weight by adopting dextrangel chromatography, and obtaining the lamb placenta polypeptide. The method is adopted for preparing the lamb placenta polypeptide, so the lamb embryo and lamb placenta active substances are reserved to the greatest degree, the comprehensive absorption by human bodies is also convenient, the process is advanced and reasonable, and the quality is controllable.

Description

technical field [0001] The invention relates to a sheep embryo and sheep placenta biological product and a preparation method thereof. Background technique [0002] Our country has a long history of using various organs, tissues and metabolites of animals for disease treatment, disease prevention and medical care. There is a complete pharmacy classic - "Shen Nong's Materia Medica", which includes bezoar, rhino horn, velvet antler, donkey-hide gelatin and other animals. Drug records. It shows that our country has already used animal drugs to process, extract, and refine in pharmaceutical technology to form relatively pure pharmaceuticals. [0003] Placenta (Placenta), also known as "Ziheche", is a commonly used traditional Chinese medicine. Modern medical research shows that: placenta contains a variety of immunoglobulins, active peptides and other biologically active substances, amino acids, minerals and other components (collectively referred to as live cells). A regulato...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K4/12C07K14/47C07K1/16
Inventor 姜雪琪姜艳
Owner HUZHOU KANGHAISI BIOLOGICAL TECH
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