Preparation method of vaccine for Edwardsiellosis of Ictalurus punctatus

A technology for channel catfish and inactivated vaccine, which is applied in the directions of microorganism-based methods, biochemical equipment and methods, microorganisms, etc., to achieve the effects of safe use, no drug residues, and scientific and rational design

Inactive Publication Date: 2012-03-14
GUANGXI INST OF FISHERIES
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Problems solved by technology

However, there is currently a lack of an effective program to prevent and treat the disease. Drugs can only play an auxiliary role in controlling the disease in the early

Method used

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Embodiment Construction

[0014] 1. Inoculate the Edwardsiella spp. isolated from the diseased fish into the tryptone soybean agar medium, and cultivate it at 28±0.5°C for 48 hours.

[0015] 2. The thalline cultured on the solid medium of step (1) is inoculated into an Erlenmeyer flask that has been autoclaved and filled with 1000ml of tryptone soybean liquid medium, and a small volume of liquid is carried out in a vibrating shaker. For activation culture, the culture temperature is 28±0.5°C, the shaker speed is 120-150 rpm, and the culture time is 20-24 hours.

[0016] 3. Inoculate the cultured thalline in the step (2) into the tryptone soybean liquid medium that has been autoclaved in the bioreactor in a ratio of 2% or 3%, the culture temperature is 28 ± 0.5 ° C, and the culture time is 24 -48 hours, when the growth of Edwardsiella spp. reached its peak, stop the reaction.

[0017] 4. Take out the bacterial liquid cultivated in step (3), add 0.2% formalin solution, and inactivate for 24-48 hours.

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Abstract

The invention provides a preparation method of a vaccine for inactivating Edwardsiella icataluri. The preparation method comprises the following steps: separating and purifying Edwardsiella icataluri separated from in vivo infected fish, carrying out small volume activation culture, carrying out enlarged culture by a bioreactor, carrying out inactivation, centrifugation and collection on Edwardsiella icataluri, extracting genomic DNA from pichia yeast, adding the genomic DNA of the pichia yeast to the completely inactivated Edwardsiella icataluri, and mixing to prepare the vaccine for inactivating Edwardsiella icataluri. The vaccine of the invention, which has the characteristics of usage safety, no medicine residual, and substantial immunization control effect, allows the generation of Edwardsiellosis icataluri of Ictalurus punctatus to be effectively prevented, and the immunization protecting rate can reach 80%. Immunizations carried out by adjustable continuous injectors are convenient, so the immunization efficiency and the dosage accuracy of the inoculation vaccine are substantially improved. The preparation method of the vaccine of the present invention has the advantages of simple operation and low cost; and the vaccine which can be used for controlling diseases of Ictalurus punctatus has a high business value.

Description

technical field [0001] The invention relates to a method for preventing and controlling fish diseases, in particular to a method for preparing an inactivated vaccine for channel catfish Edwards disease. Background technique [0002] Channel catfish is native to the Americas. This fish has the characteristics of wide adaptability, miscellaneous food habits, fast growth, fresh and tender meat, high meat yield, no intermuscular spines, and simple processing. It was introduced from the United States in 1984 to our country for breeding. one of the most important breeds. With the decline of global marine capture fishery resources, the demand for aquaculture products in the international market is increasing. According to incomplete statistics, the annual output of channel catfish in my country is about 100,000 tons, and the export volume in 2009 was 19,000 tons, with an export value of 70 million US dollars. It is one of the main varieties of my country's aquatic product exports....

Claims

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Application Information

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IPC IPC(8): A61K39/02C12N1/20A61P31/04C12R1/01
Inventor 梁万文黄婷李莉萍陈福艳张彬雷爱莹王瑞
Owner GUANGXI INST OF FISHERIES
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