Preparation method of vaccine for Edwardsiellosis of Ictalurus punctatus

Abstract

The invention provides a preparation method of a vaccine for inactivating Edwardsiella icataluri. The preparation method comprises the following steps: separating and purifying Edwardsiella icataluri separated from in vivo infected fish, carrying out small volume activation culture, carrying out enlarged culture by a bioreactor, carrying out inactivation, centrifugation and collection on Edwardsiella icataluri, extracting genomic DNA from pichia yeast, adding the genomic DNA of the pichia yeast to the completely inactivated Edwardsiella icataluri, and mixing to prepare the vaccine for inactivating Edwardsiella icataluri. The vaccine of the invention, which has the characteristics of usage safety, no medicine residual, and substantial immunization control effect, allows the generation of Edwardsiellosis icataluri of Ictalurus punctatus to be effectively prevented, and the immunization protecting rate can reach 80%. Immunizations carried out by adjustable continuous injectors are convenient, so the immunization efficiency and the dosage accuracy of the inoculation vaccine are substantially improved. The preparation method of the vaccine of the present invention has the advantages of simple operation and low cost; and the vaccine which can be used for controlling diseases of Ictalurus punctatus has a high business value.

Description

technical field [0001] The invention relates to a method for preventing and controlling fish diseases, in particular to a method for preparing an inactivated vaccine for channel catfish Edwards disease. Background technique [0002] Channel catfish is native to the Americas. This fish has the characteristics of wide adaptability, miscellaneous food habits, fast growth, fresh and tender meat, high meat yield, no intermuscular spines, and simple processing. It was introduced from the United States in 1984 to our country for breeding. one of the most important breeds. With the decline of global marine capture fishery resources, the demand for aquaculture products in the international market is increasing. According to incomplete statistics, the annual output of channel catfish in my country is about 100,000 tons, and the export volume in 2009 was 19,000 tons, with an export value of 70 million US dollars. It is one of the main varieties of my country's aquatic product exports....

AI Technical Summary

Problems solved by technology

However, there is currently a lack of an effective program to prevent and treat the disease. Drugs can only play an auxiliary role in controlling the disease in the early stage. However, with the emergence and accumulation of drug resistance and drug resistance, the current status of the disease is almost "There is no cure or prevention"