Three-dimensional real time super-resolution digital holography recording system

A digital holography and recording system technology, applied in microscopes, optics, instruments, etc., can solve the problem of not being able to have high time and high spatial resolution at the same time, and achieve spatial and temporal resolution, high resolution, and large field of view. and depth of field effects

Inactive Publication Date: 2015-03-11
KUNMING UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to provide a three-dimensional real-time super-resolution digital holographic recording system, which uses wavelength division, angle Division, time division and polarization multiplexing hybrid technology, simultaneously record four holograms containing two transient moments and high and low frequency information of each transient moment in the same frame of the digital camera, so that the digital holographic microscope system has both Features of high temporal and spatial resolution, large field of view, long working distance and large depth of field

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Embodiment 1

[0027] Embodiment 1: as figure 1 , 2 As shown, the three-dimensional real-time super-resolution digital holographic recording system includes two parts: an incident light generation system and a recording system. The incident light generation part includes a femtosecond pulse laser 1, a frequency doubling crystal 2 that changes the wavelength of the incident laser pulse, a dichroic mirror 3 that can separate two different wavelength laser pulses from the frequency doubling crystal 2 and has an output time difference, and a mirror 4 , 5, and a non-polarizing beam splitter 6; the recording system includes a polarization beam splitter that decomposes each incident laser pulse entering the system (from the non-polarizing beam splitter 6) into linearly polarized beams in the horizontal X direction and vertical Y direction The mirror 7 is located between the polarizing beam splitter 7 and the reflector 9, and the non-polarizing beam splitter 8 that decomposes the polarized light fr...

Embodiment 2

[0058] Embodiment 2: as figure 1 , 2 As shown, the three-dimensional real-time super-resolution digital holographic recording system is the same as that in Embodiment 1. The laser 1 used is an ORANGE-type ytterbium-doped fiber oscillator with an output laser pulse center wavelength of 1030nm and a pulse time interval of less than 100fs. The frequency doubling crystal 2 can double the frequency of the original input pulse laser, that is, the laser wavelength passing through it KTP potassium titanyl phosphate that has become 1 / 2 times the original wavelength (size: 2 mm×5 mm×5 mm, the crystal response wavelength range is 350-4500nm, and the frequency doubling efficiency is about 70%). The generated laser pulse optical delay system is realized by a dichroic mirror 3, two reflective mirrors 4, 5 placed symmetrically on the reflective surface, and a non-polarizing beam splitter 6. The dichroic mirror has dichroic The spectroscopic plate has high reflectivity for light with a wave...

Embodiment 3

[0059] Embodiment 3: as figure 1 , 2 As shown, the three-dimensional real-time super-resolution digital holographic recording system is the same as that in Embodiment 1. The laser 1 used is a Clarke-MXR CPA2010 femtosecond laser oscillator with an output laser pulse center wavelength of 775nm and a pulse time interval of less than 150fs. The frequency doubling crystal 2 can generate another wavelength while passing through the 775nm high-frequency laser pulse. LBO lithium triborate crystal (dimension: 5mm×3mm×8mm, crystal response wavelength range is 160-2600nm, frequency doubling efficiency is about 30%) with low-frequency laser pulse at 387nm; the optical delay of the generated laser pulse is composed of a The dichroic mirror 3, a roof prism and a non-polarizing beam splitter 6 realize that the dichroic mirror is a dichroic beam splitter, which has a high reflectivity to the light of 387nm wavelength and has a high reflectivity to the light of 775nm. The higher transmittan...

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Abstract

The invention relates to a three-dimensional real time super-resolution digital holography recording system, which belongs to the field of three-dimensional microscopic imaging technology and comprises an incident light generation system and a recording system. The incident light generation system comprises a femtosecond pulse laser, a frequency doubling crystal, a dichroscope, an optical path delay system and an unpolarized beam splitter. Said recording system comprises a microobjective and a series of polarized beam splitter and unpolarized beam splitter, a reflector, a digital camera and a computer holography image processing and reproducing system. With the technologies of wavelength division, angle division, time division and polarization division multiplexing and with the optical frequency doubling crystal, the lasers sent from the laser device can be divided into two lasers with different wavelengths to enter the recording system; then a digital holography record for the three-dimensional real time super-resolution imaging can be realized in one frame image of Charge Coupled Device (CCD) through a series of beam splitting, high resolution, large view field and large depth of field. The invention has the advantages of simple system and more information records, which can simultaneously improve the space resolution and time resolution and can be widely applied to the three-dimensional real time high-resolution imaging for the microscopic object.

Description

technical field [0001] The invention relates to a three-dimensional real-time super-resolution digital holographic recording system. Specifically, it is an imaging system that uses digital holographic microscopic technology to realize three-dimensional real-time ultra-high spatial resolution, and belongs to the technical field of three-dimensional microscopic imaging. Background technique [0002] Digital holographic microscopy is a new microscopic imaging technology developed in recent years. It has been applied in biological cell imaging, detection of MEMS devices and micro-optical devices, deformation and vibration measurement of tiny objects. It uses digital cameras such as CCD or CMOS to record the hologram formed by the interference of the object and the reference light, and uses the computer simulation diffraction process to reproduce the wavefront information (phase and amplitude) of the recorded microscopic object. The data information of the two can be direc...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G03H1/12G03H1/22G02B21/36
Inventor 袁操今马骏
Owner KUNMING UNIV OF SCI & TECH
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