Application of monoclonal antibody for Riemerella anatipestifer in preparation of immune colloidal gold test paper
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A technology of Riemerella anatipestifer and Riemerella anatipestifer is applied in the field of antibody engineering and immune colloidal gold method to detect Riemerella anatipestifer, achieving the effect of sensitive detection method and wide application value
Inactive Publication Date: 2012-05-02
YANGZHOU UNIV
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However, there is no colloidal gold test strip method for antigen detection. It is necessary to carry out relevant research to meet the needs of the field and production practice. The diagnosis of the disease can provide a fast and effective tool
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[0013] Biological material in the present invention can be obtained through the following ways:
[0014] 1, 2, 3, 11 four serotypes of RA: References 1, 3 types: Yan Tianjiu. Epidemiological investigation and control of Riemerella anatipestifer in Baise City [D]. Guangxi University, 2007. Type 2 and Type 11: Yang Yong. Isolation, Identification and Pathogenicity of Riemerella anatipestifer in Some Areas of Jiangsu[D], Yangzhou University, Professional Master Thesis, 2008. Yun Shuili, Yang Yong, Wang Xiaobo et al. Isolation, identification and pathogenicity of Riemerella anatipestifer type 11[J]. Journal of Preventive Veterinary Medicine, 2009,8:605-609.
[0015] Pasteurella (domestic standard virulent strain C48-1, purchased from China Veterinary Drug Control Institute).
[0017] Escherichia coli (DH5α, purchased from Promega).
[0018] 1. Preparation of monoclonal antibody
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Abstract
The invention belongs to the field of biotechnology and particularly relates to a monoclonal antibody for Riemerella anatipestifer and an application thereof. The monoclonal antibody 5G7 has a preservation number of CGMCC (China General Microbiological Culture Collection Center) No.5165. The invention also discloses an application of the monoclonal antibody in preparation of an immune colloidal gold test paper for detecting the Riemerella anatipestifer. According to the invention, the immune colloidal gold test paper can be used to not only detect cause of disease directly but also detect RA (Riemerella Anatipestifer) strains of different serotypes 1, 2, 3 and 11 at the same time, so that the immune colloidal gold test paper has higher application value.
Description
technical field [0001] The invention belongs to the field of biotechnology and relates to antibody engineering technology, specifically monoclonal antibodies for identifying different serotypes of Riemerella anatipestifer and an immune colloidal gold method based on this to detect Riemerella anatipestifer . Background technique [0002] Riemerella anatipestifer (RA) is the pathogenic bacterium that causes infectious serositis in ducks. It mainly infects a variety of poultry, mainly waterfowl, and causes acute or chronic infectious diseases. The diseased ducks often show lethargy, crooked neck, lameness, yellow-green loose feces, serous secretions from the eyes and nose, and then neurological symptoms such as convulsions and opisthotonos. The lesions are characterized by fibrinous pericarditis, perihepatitis, air sacculitis, caseous salpingitis and meningitis. At present, serum types 1, 2, 3, 4, 5, 6, 7, 8, 10, 11, 13, 14 and 4 unnamed types have been reported successively ...
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