Compound mulberry preparation capable of improving superoxide dismutase activity
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A technology of superoxide and dismutase, which can be used in medical preparations containing active ingredients, antidote, food science, etc., and can solve problems such as side effects
Inactive Publication Date: 2012-06-27
SHANDONG UNIV AT WEIHAI
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Synthetic antioxidants have good antioxidant properties, but they have certain side effects
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Embodiment 1
[0019] 1, a kind of preparation method of the compound mulberry preparation that can improve superoxide dismutase activity is characterized in that comprising the following steps:
[0020] (1) The weight ratio of raw materials is: mulberry fruit 9g, medlar fruit 6g, Porphyridum 10g;
[0021] (2) Add 8 times the amount of 70% ethanol to extract 8 times the amount of mulberry and medlar at room temperature, then seal and ultrasonicate for 30 minutes at a temperature of 30°C and a power of 80W, then filter with gauze, and use 6 times and 4 times the amount of filter residues respectively Repeat the extraction with 70% ethanol once, and filter to obtain the filtrate;
[0022] (3) The filtrate in (2) is merged, reclaims ethanol, obtains mulberry fruit, wolfberry extract;
[0023] (4) Centrifuge Porphyridium algae with a 5000r / min centrifuge for 10min, then suspend it in pH 7.4, 50mmol / L phosphate buffer, perform ultrasonic crushing, break for 5s, interval 5s, 20 times, use 10000r / ...
Embodiment 2
[0027] 1, a kind of preparation method of the compound mulberry preparation that can improve superoxide dismutase activity is characterized in that comprising the following steps:
[0028] (1) The weight proportion of raw material medicine is: 12g of mulberry fruit, 9g of medlar, 20g of Porphyridium coccus;
[0029] (2) Add 10 times the amount of 70% ethanol to mulberry fruit and medlar and extract at room temperature for 10 hours, then seal and ultrasonicate for 30 minutes at a temperature of 30°C and a power of 80W, then filter with gauze, and use 6 times and 4 times the amount of filter residues respectively Repeat the extraction with 70% ethanol once, and filter to obtain the filtrate;
[0030] (3) The filtrate in (2) is merged, reclaims ethanol, obtains mulberry fruit, wolfberry extract;
[0031] (4) Centrifuge Porphyridium algae with a 5000r / min centrifuge for 10min, then suspend it in pH 7.4, 50mmol / L phosphate buffer, perform ultrasonic crushing, break for 5s, interva...
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Abstract
The invention belongs to the technical field of processing of health-care products and relates to a compound mulberry preparation capable of improving superoxide dismutase activity. The preparation method for the compound mulberry preparation comprises the following steps of: extracting 12g of mulberry and 9g of medlar in ethanol of which the concentration is 70 percent and the amount is 10 timesthat of the mulberry and the medlar at room temperature for 10 hours, sealing and crushing the extract with ultrasonic waves for 30 minutes, filtering the extract, and extracting the filtration residue with ethanol of which the concentration is 70 percent and the amount is 6 times and 4 times that of the filtration residue again for one time respectively; combining the filtrates, reclaiming the ethanol to obtain an extract of the mulberry and medlar; centrifuging 20g of porphyridium cruentum with a centrifugal for 10 minutes, suspending the porphyridium cruentum in 50mmol / L of phosphate buffer of which the pH value is 7.4, crushing the porphyridium cruentum with ultrasonic waves, centrifuging the crushed porphyridium cruentum with a high-speed centrifugal for 15 minutes to obtain a liquidsupernatant, and refrigerating and drying the liquid supernatant to obtain a dried product; and uniformly mixing the dried product and the extract of the mulberry and medlar to obtain the mulberry preparation. The mulberry preparation can be applied to a natural antioxidant which is newly developed in fields, such as food, medicament and the like, and contributes to industrial production.
Description
technical field [0001] The invention belongs to the technical field of health food processing, and the invention relates to a compound mulberry preparation capable of improving the activity of superoxide dismutase. The product of the invention can be used to develop new natural antioxidants in the fields of food, medicine and the like, and the product of the invention is beneficial to industrial production. Background technique [0002] Mulberry is the dried fruit ear of Morus alba L., a plant of the Moraceae family. Sweet, sour, cold. GUIXIN, liver, kidney channel. Enriches blood and nourishes yin, promotes fluid production and moisturizes dryness. Contains glucose, fructose, tannin, malic acid, rutin, anthocyanin, carotene, niacin, linoleic acid, vitamin B, vitamin C, mucus, cyanidin, calcium, etc. Modern pharmacology has proved that it can increase the weight of immune organs, enhance non-specific immune function, increase humoral immunity, significantly promote T cel...
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