34 results about "Porphyridium cruentum" patented technology

This invention focuses on a novel process in which Porphyridium cruentum biomass first undergoes a stage of cellular disruption and subsequently stages of recovery and purification in order to achieve the purified B-phycoerythin (BFE) protein dye, using isoelectric precipitation and two-aqueous-phase systems. The steps of recovery and purification include isoelectric precipitation followed by a step of liquid / liquid extraction by means of two-aqueous-phase systems that use polyethylene glycol (PEG) and phosphate salts. The BFE protein dye obtained in the two-aqueous-phase extraction step undergoes an ultrafiltration step in order to remove the polymer (PEG) and to obtain a dye with a purity greater than 4.0 defined as the relationship between the absorbencies at 545 and 280 nm (BFE purity=Abs 545 nm / Abs 280 nm).

The invention belongs to the technical field of bio-adsorbent based treatment for metal ions in wastewater, and in particular relates to a method for absorbing metal ions through exopolysaccharide of porphyridium cruentum. The method is characterized by comprising the steps of culturing porphyridium cruentum; extracting exopolysaccharide of porphyridium cruentum; absorbing the metal ions by the exopolysaccharide of porphyridium cruentum; and desorbing the exopolysaccharide of porphyridium cruentum. The method has the beneficial effects that liquid in a liquid storing tank can be prevented from overflowing; in case of fault in a third level probe, an alarm alarms, and therefore, the material waste and fault can be effectively avoided, and as a result, the filling quality can be ensured.

The invention provides an eye care product. The eye care product contains the following active ingredients: centella asiatica extract, porphyridium cruentum extract, corn poppy extract, humectant, grease, surfactant, alcohols, gel matrix, ph modifier, wetting agent and water. Compared with the prior art, the functional components of the eye care product are centella asiatica extract, porphyridium cruentum extract and corn poppy extract; under the synergistic effect of centella asiatica extract, porphyridium cruentum extract and corn poppy extract, the eye care product can remarkably increase the elasticity of skin, the eye skin problem of black eyes, pouch, wrinkles and the like can be effectively solved, and the eye care product also has the function of improving lipid granules and edema; in addition, by matching with other ingredients in the eye care product, the eye care product has a better moisturizing effect, and the moisture of eye skin can be increased.

The invention belongs to the technical field of processing of health-care products and relates to a compound mulberry preparation capable of improving superoxide dismutase activity. The preparation method for the compound mulberry preparation comprises the following steps of: extracting 12g of mulberry and 9g of medlar in ethanol of which the concentration is 70 percent and the amount is 10 timesthat of the mulberry and the medlar at room temperature for 10 hours, sealing and crushing the extract with ultrasonic waves for 30 minutes, filtering the extract, and extracting the filtration residue with ethanol of which the concentration is 70 percent and the amount is 6 times and 4 times that of the filtration residue again for one time respectively; combining the filtrates, reclaiming the ethanol to obtain an extract of the mulberry and medlar; centrifuging 20g of porphyridium cruentum with a centrifugal for 10 minutes, suspending the porphyridium cruentum in 50mmol / L of phosphate buffer of which the pH value is 7.4, crushing the porphyridium cruentum with ultrasonic waves, centrifuging the crushed porphyridium cruentum with a high-speed centrifugal for 15 minutes to obtain a liquidsupernatant, and refrigerating and drying the liquid supernatant to obtain a dried product; and uniformly mixing the dried product and the extract of the mulberry and medlar to obtain the mulberry preparation. The mulberry preparation can be applied to a natural antioxidant which is newly developed in fields, such as food, medicament and the like, and contributes to industrial production.

This invention focuses on a novel process in which Porphyridium cruentum biomass first undergoes a stage of cellular disruption and subsequently stages of recovery and purification in order to achieve the purified B-phycoerythin (BFE) protein dye, using isoelectric precipitation and two-aqueous-phase systems. The steps of recovery and purification include isoelectric precipitation followed by a step of liquid / liquid extraction by means of two-aqueous-phase systems that use polyethylene glycol (PEG) and phosphate salts. The BFE protein dye obtained in the two-aqueous-phase extraction step undergoes an ultrafiltration step in order to remove the polymer (PEG) and to obtain a dye with a purity greater than 4.0 defined as the relationship between the absorbencies at 545 and 280 nm (BFE purity=Abs 545 nm / Abs 280 nm).

The invention discloses a skin care cleansing water. The cleansing water is composed of the following ingredients according to a certain mass ratio: 10-20 parts of galanga fruit oil, 10-15 parts of grape seed oil, 8-12 parts of Lavender essential oil, 7-9 parts of olive oil essence, 3-7 parts of green tea essential oil, 4-8 parts of peppermint extract, 6-9 parts of chamomile extract, 5-8 parts of Porphyridium extract, 8-10 parts Aloe vera, 1-3 parts of calendula, 4-6 parts of cucumber juice, 1-3 parts of vitamin A, 3-5 parts of glycosyl trehalose. The cleansing water of the present invention has a high content of natural ingredients and is less irritating to the skin, and the used malula oil and grape seed oil make the cleansing power strong; at the same time, the cleansing water of the present invention moisturizes, maintains skin elasticity, and is moist and gentle , Aloe vera has a powerful moisturizing function, vitamin A can make the skin soft and tender, has the effect of anti-wrinkle and anti-wrinkle, and glycosyl trehalose has the effect of anti-drying.

The invention discloses a method for harvesting porphyridium cruentum based on foam floatation. According to the method, rhamnoilpid is used in cooperation with a foaming agent, a floating product is collected in the mode of foam floatation, and finally the porphyridium cruentum is obtained. The method is suitable for treating porphyridium cruentum liquor within the range of 6.0*106 cells / L-2.0*109 cells / L as algal cell density in the porphyridium cruentum liquor, the concentration multiple of the algal cell density in the porphyridium cruentum liquor before treatment can reach 102 times, one or less hour is consumed for treatment, and the recovery rate of the porphyridium cruentum can reach 90% or above; floatation can be performed in a single-trough floating machine, in this way, investment cost is low, and industrialization is convenient.

The invention provides anti-bacterial oil-proof paper and a preparation method thereof. The preparation method comprises the following steps: (1) adding zein into water so as to prepare a gelatinizedzein solution; (2) dissolving Konjak refined powder, Artemisia sphaerocephala Krasch gum, edible polyvinylpyrrolidone, propolis powder and waterborne stearic acid in remained water, carrying out uniform mixing under stirring, and carrying out standing for 2 h or above; (3) adding the mixed solution obtained in the step (2), tributyl citrate, glyceryl triacetate, Nostoc Sphaeroids Kutz polysaccharide and Porphyridium cruentum polysaccharide into the gelatinized zein solution, and carrying out uniform mixing under stirring; and (4) coating a surface sizing agent onto base paper with a surface sizing machine, drying paper at a temperature of 100 DEG C, then calendering the sized paper, and carrying out drying so as to balance moisture. The anti-bacterial oil-proof paper provided by the invention has oil-proof performance and antibacterial performance.

Disclosed skin-care makeup-removing water is prepared from the following raw materials in parts by weight: 4-10 parts of gardenia extracting liquid, 20-30 parts of lanolin, 10-18 parts of porphyridium cruentum extractive, 15-25 parts of amnion liquid, 3-6 parts of kathon, 40-70 parts of deionized water, 4-7 parts of olive oil, 1-4 parts of methylisothiazolinone, 3-7 parts of rhizoma alismatis, 3-5 parts of sodium acetylhyaluronate, 6-9 parts of hydrogenated starch hydrolysates, 15-20 parts of an amino-acid serial surfactant, 4-7 parts of phenoxyethanol and 1-3 parts of Q10 coenzyme. The provided skin-care makeup-removing water is capable of rapidly removing oil without damaging skin, emits light fragrance and is mild in properties.

The invention provides a method for preparing phycoerythrin through combination of a centrifugal method and anion exchange chromatography media. The method comprises the following steps of using fresh porphyridium cruentum as a raw material, performing centrifugation, mixing porphyridium cruentum paste with a phosphate buffer solution according to a certain proportion, and then performing ultrasonic crushing treatment so as to obtain a wall-breaking solution; and then performing refrigerated centrifugation on the wall-breaking solution, taking a supernatant, performing ultrafiltration on the supernatant with a membrane bag of which the molecule interception is 50-60kD for 4-6 times, adding strong anion chromatography media pretreated by the phosphate buffer solution, performing refrigerated centrifugation twice, and taking a supernatant so as to obtain the phycoerythrin of which the purity A<545> / A<280> is greater than 4.0. The preparation method disclosed by the invention is simple and convenient in technology, low in cost, good in purification effects, high in protein recovery rate and easy to amplify, and has a good popularization value and good application prospects.

The invention belongs to the field of health-care foods, and discloses composite algae noodles capable of recuperating digestive tracts. The composite algae noodles comprise the following components of 40-60 parts of wheat flour, 1-5 parts of nostoc punctiforme, 1-5 parts of ishige okamurae and 1-5 parts of porphyridium cruentum. According to the composite algae noodles disclosed by the invention,the nostoc punctiforme, the ishige okamurae and the porphyridium cruentum are compounded into the composite algae noodles, so that rich nutrient substances contained in microalgae can be complemented; through the physiological activity effect of the microalgae, balance of intestinal flora can be regulated, hurt to the digestive tracts by hazardous substances can be reduced, free radicals can be effectively eliminated, the incidence rate of diseases of the digestive tracts can be reduced, and the effect of improving the environment of the digestive tracts can be achieved.

The invention provides a method for preparing high-purity phycoerythrin through hydrophobic chromatography. The method comprises the following steps of using fresh porphyridium cruentum as a raw material, uniformly mixing porphyridium cruentum paste with a phosphate buffer solution according to a certain proportion, and performing repeated freezing and thawing for 5-7 times so as to obtain a wall-breaking solution; performing refrigerated centrifugation on the wall-breaking solution, taking a supernatant, and performing ultrafiltration on the supernatant with a membrane bag of which the molecule interception is 50-60kD for 4-6 times so as to obtain a coarse phycoerythrin extract; and then loading samples of the coarse phycoerythrin extract to a hydrophobic chromatography column (Phenyl Sepharose Fast Flow) for gradient elution, collecting flow-out components of which A<545> / A<280> is greater than 4.0, and performing freeze drying so as to obtain purified phycoerythrin. The preparation method disclosed by the invention is good in purification effects, high in protein recovery rate and simple and convenient in technology, and has a good popularization value and good application prospects.

The invention belongs to the technical field of health-care food processing, and relates to a compound foreign ginseng preparation capable of improving activity of superoxide dismutase. The compound foreign ginseng preparation is prepared by the following steps of: adding 12g of foreign ginseng and 9g of pseudostellaria root into 70 percent ethanol in an amount which is 10 times of amount of the foreign ginseng and the pseudostellaria root to lixiviate for 10 hours; performing hermetical ultrasonic processing for 30 minutes; filtering; repeatedly extracting filter residue by using 70 percent ethanol in an amount which is 6 times the amount of the filter residue and in an amount which is 4 times the amount of the filter residue respectively; combining filtrates; recovering the ethanol to obtain foreign ginseng and the pseudostellaria root extract; centrifuging 20g of porphyridium cruentum by using a centrifuge for 10 minutes; suspending in 50mmol / L phosphoric acid buffer solution with pH of 7.4; performing ultrasonic crushing; centrifuging for 15 minutes by using a high-speed freezing centrifuge to obtain supernatant; freeze-drying to obtain dry product; and uniformly mixing the dry product with the foreign ginseng and the pseudostellaria root extract to obtain the compound foreign ginseng preparation. The product can be used in the fields of foods, medicaments and the like for developing new natural antioxidants and contributes to industrial production.

The invention discloses multiple-effect activation eye activating concentrate and a preparation method thereof. The multiple-effect activation eye activating concentrate comprises the following raw materials of a yeast fermentation product extract, acetyl hexapeptide-8, acetyl tetrapeptide-2, acetylated sodium hyaluronate, phoenix dactylifera seeds, a porphyridium cruentum extract, an emulsifyingagent, an emollient, a thickening agent and a preservative. The multiple-effect activation eye activating concentrate prepared by the preparation method disclosed by the invention has relatively strong antioxygenic property, can remove redundant free radicals in a body, activate skin cells, promote metabolism of the skin cells, reduce loss of collagen and elastin, improve black eyes, reduce eye wrinkles, improve eye skin compactness, improve skin roughness and resist aging, and is durable and stable in efficacy, and mild and non-irritant in action.

The invention belongs to the technical field of the research and development of medicine products, and relates to a method for preparing a compound ginseng preparation and the antioxidation activity of the compound ginseng preparation. The method comprises the following steps of: adding 70 percent ethanol into ginseng and szechuan tangshen root, leaching at room temperature, performing ultrasound at the temperature of 30 DEG C under the condition of power of 80 W for 30 minutes, filtering, extracting filter residues once again by using 70 percent ethanol in an amount 6 times and 4 times that of the filter residues respectively, and filtering to obtain filtrate; mixing the filtrate, and recovering the ethanol to obtain a ginseng and szechuan tangshen root extract; centrifuging porphyridium cruentum by using a centrifugal machine of which the rotation speed is 5,000 revolutions / minute for 10 minutes, suspending in phosphate buffer of which the concentration is 50 mmol / L and the pH value is 7.4, performing ultrasonication, centrifuging by using a high-speed freezing centrifugal machine to obtain supernate, and freezing and drying to obtain a dry substance; and mixing the dry substance and the ginseng and szechuan tangshen root extract uniformly to obtain the compound ginseng preparation. The compound ginseng preparation has an antioxidation effect.

The invention belongs to the technical field of research and development of medical products, and relates to a preparation method for a compound Chinese magnoliavine fruit preparation and antioxidant activity thereof. The method comprises the following steps of: adding Chinese magnoliavine fruit and astragalus root into 70 percent ethanol to lixiviate at room temperature; performing ultrasonic processing for 30 minutes at the temperature of 30 DEG C and at the power of 80 W; filtering; repeatedly extracting filter residue by using 70 percent ethanol in an amount which is 6 times the amount ofthe filter residue and in an amount which is 4 times the amount of the filter residue respectively; filtering; combining filtrates; recovering the ethanol to obtain Chinese magnoliavine fruit and astragalus root extract; centrifuging porphyridium cruentum by using a centrifuge of 5,000r / min for 10 minutes; suspending in 50mmol / L phosphoric acid buffer solution with pH of 7.4; performing ultrasonic crushing; centrifuging by using a high-speed freezing centrifuge to obtain supernatant; freeze-drying to obtain a dry product; and uniformly mixing the dry product with the Chinese magnoliavine fruit and astragalus root extract to obtain the compound Chinese magnoliavine fruit preparation. The product has an antioxidant effect.

The invention discloses an alga-containing composition used for a cosmetic. The composition in dry weight contains the following components in percentage by mass: 0.003-0.125% of an alga mixture, 0.001-0.050% of radix ophiopogonis extract, 0.100-25.000% of betaine and the balance of deionized water, wherein the alga mixture is composed of porphyridium cruentum extract, bladderwrack extract and chondrus crispus extract, the content of the porphyridium cruentum extract is 0.001-0.005%, the content of the bladderwrack extract is 0.001-0.030%, and the content of the chondrus crispus extract is 0.001-0.090%. The invention also discloses a cosmetic composition applying the composition used for the cosmetic. The alga-containing composition used for the cosmetic has the effects of nature, high efficiency, easiness in absorption by virtue of a human body and long moisture preserving time and is applicable to manufacturing of various moisture preserving type skincare products.

The invention discloses an application of a compound 3,3',5,5'-tetrabromo-2,2'-biphenol in the aspect of algal inhibition. Algae removal spectrum test proves that the compound has excellent inhibitioneffect on eleven algae comprising thalassiosira pseudonana, chaetoceros mulleri, prorocentrum minimum, amphidinium carterae, nannochloropsis oculata, prochlorococcus, dunaliella salina, phaeodactylumtricornutum, chlorella vulgaris, tetraselmis subcordiformis, porphyridium cruentum, etc. The compound has great significance on treatment of red tide, algal bloom and the like natural hazard and canprevent eutrophication of water body.

The invention belongs to the technical field of alga culture and discloses a technology for producing purified phycoerythrobilin. The technology comprises the following steps: step 1) preparing a porphyridium cruentum seed solution; step 2) carrying out large-scale culture; and step 3) separating and purifying. By adopting the technology provided by the invention, the content of phycoerythrin in porphyridium cruentum is improved and the phycoerythrin is separated and purified.

The invention provides a method used for preparing high-purity phycoerythrin from porphyridium cruentum. The method is simple in operation, and low in cost, and is convenient for large-scale industrialized production. Compared with the prior art, the method used for preparing high-purity phycoerythrin via chitosan adsorption comprises following advantages: a step of column chromatography is avoided; operation is convenient; cost is low; recovery rate is high; purity is high; and verification using laboratory amplify production equipment is carried out. The purity of phycoerythrin obtained via the method A<545> / A<280> is higher than 4.0; analytical grade requirements are satisfied; and foundation is provided for applications of phycoerythrin in the fields such as biomedical analysis and immunochemistry.

The invention belongs to the technical field of processing of health-care foods, and relates to a compound acanthopanax root preparation capable of improving activity of superoxide dismutase. A preparation method of the compound acanthopanax root preparation comprises the following steps of: adding 12g of acanthopanax root and 9g of thinleaf milkwort root-bark into 70 percent ethanol which is 10 times of the total amount of the acanthopanax root and the thinleaf milkwort root-bark for leaching at the room temperature for 10 hours, performing hermetic ultrasonic treatment for 30 minutes, filtering, and repeatedly extracting filter residues with two kinds of 70 percent ethanol, which are 6 times and 4 times of the total amount of the acanthopanax root and the thinleaf milkwort root-bark once respectively; combining filtrates and recovering ethanol to obtain acanthopanax root and thinleaf milkwort root-bark extracts; centrifuging 20g of porphyridium cruentum with a centrifugal machine for 15 minutes, suspending in 50 mmol / L of phosphoric acid buffer solution of which pH is 7.4, ultrasonically smashing, centrifuging by using a high-speed refrigerating centrifugal machine for 15 minutes to obtain a supernatant, and chilling and drying to obtain a dry substance; and uniformly mixing the dry substance with the acanthopanax root and thinleaf milkwort root-bark extracts to obtain the compound acanthopanax root preparation. The compound acanthopanax root preparation can be applied in the field of foods, medicaments and the like for developing a new natural antioxidant, and is beneficial to industrial production.

The invention discloses a method for producing a makeup removing solution. The method comprises the following steps: (1) taking 10 g of Gardenia jasminoides extract, 8 g of Porphyridium cruentum extract, 6 g of methylparaben, 8 g of mannitol, 12 g of butyl stearate, 5 g disodium ethylenediaminetetraacetate and 15g sorbitol, adding the taken materials into a container, and fully stirring the materials for 5min; (2) adding 20g of crocodile oil and 40 g of water, heating the obtained solution to 40 DEG C, and standing the solution for 20 min; and (3) adding 12 g of alpha hydroxy acid, pouring theobtained solution into a distillation vessel, distilling the solution for 1 h, and taking out the distilled solution. The makeup removing solution has the advantages of simple manufacturing process,low cost, completeness in makeup removal, no residual, and harmlessness to skins, and can effectively prevent skin aging, nourish skins, moisturize and rejuvenate the skins, smooth pores and prevent hair follicle clogging.

The invention discloses a giant salamander feed. The giant salamander feed comprises the following components in parts by weight: 10 to 20 parts of crab meat, 20 to 25 parts of fishbone dust, 5 to 15parts of compound amino acids, 5 to 10 parts of plantain herb, 5 to 10 parts of verbena officinalis, 10 to 15 parts of Emblica officinalis pulp, 5 to 10 parts of porphyridium cruentum, 5 to 10 parts of laurate, 10 to 20 parts of soybean meal and 5 to 15 parts of complex vitamins. The invention also discloses a preparation method of the feed. The preparation method comprises the following steps: (1) treatment of Emblica officinalis; (2) preparation of compound amino acids; (3) preparation of complex vitamins; (4) pretreatment of raw materials; and (5) pelleting to obtain the giant salamander feed. The formula of the giant salamander feed is easy to obtain, the giant salamander feed is free of toxic action and is low in price, and the preparation method of the feed is simple, high in operability and low in preparation cost and is suitable for wide popularization and application; the physiological effects such as the immunity of the organism can be improved, the giant salamander are prevented from being infected by viruses, the appetite of the giant salamanders can be increased through fruit fragrance, and rapid growth of the giant salamanders is promoted.

The invention belongs to the technical field of processing of health-care food, and relates to a compound Siberian solomonseal rhizome preparation capable of improving the vigor of superoxide dismutase. A method for preparing the compound Siberian solomonseal rhizome preparation comprises the following steps of: adding 70 percent ethanol in an amount which is 10 times that of 12 grams of Siberian solomonseal rhizome and 9 grams of largehead atractylodes rhizome, leaching at room temperature for 10 hours, performing hermetic ultrasound for 30 minutes, filtering, extracting filter residues once again from 70 percent ethanol in an amount which is 6 times and 4 times that of the filter residues respectively; mixing the filtrate, and recovering the ethanol to obtain a Siberian solomonseal rhizome extract and a largehead atractylodes rhizome extract; centrifuging 20 grams of porphyridium cruentum for 10 minutes by using a centrifugal machine, suspending in 50 mmol / L of phosphate buffer, of which the pH value is 7.4, performing ultrasonication, centrifuging for 15 minutes by using a high-speed freezing centrifugal machine to obtain supernate, and freezing and drying to obtain a dry substance; and mixing the dry substance with the Siberian solomonseal rhizome extract and the largehead atractylodes rhizome extract uniformly to obtain the compound Siberian solomonseal rhizome preparation. The compound Siberian solomonseal rhizome preparation can be used for developing new natural antioxidants in fields of food, medicines and the like and is favorable for industrial production.

The invention discloses a method for harvesting porphyridium cruentum based on foam floatation. According to the method, rhamnoilpid is used in cooperation with a foaming agent, a floating product is collected in the mode of foam floatation, and finally the porphyridium cruentum is obtained. The method is suitable for treating porphyridium cruentum liquor within the range of 6.0*106 cells / L-2.0*109 cells / L as algal cell density in the porphyridium cruentum liquor, the concentration multiple of the algal cell density in the porphyridium cruentum liquor before treatment can reach 102 times, one or less hour is consumed for treatment, and the recovery rate of the porphyridium cruentum can reach 90% or above; floatation can be performed in a single-trough floating machine, in this way, investment cost is low, and industrialization is convenient.

The invention belongs to the technical field of the research and development of medical products, and relates to a method for preparing a compound integripetal rhodiola herb preparation and the antioxidation activity of the compound integripetal rhodiola herb preparation. The method comprises the following steps of: adding 70 percent ethanol into integripetal rhodiola herb and bupleurum, leaching at room temperature, performing ultrasound at the temperature of 30 DEG C under the condition of power of 80 W for 30 minutes, filtering, extracting filter residues once again by using 70 percent ethanol in an amount 6 times and 4 times that of the filter residues respectively, and filtering to obtain filtrate; mixing the filtrate, and recovering the ethanol to obtain a integripetal rhodiola herb and bupleurum extract; centrifuging porphyridium cruentum by using a centrifugal machine of which the rotation speed is 5,000 revolutions / minute for 10 minutes, suspending in phosphate buffer of which the concentration is 50 mmol / L and the pH value is 7.4, performing ultrasonication, centrifuging by using a high-speed freezing centrifugal machine to obtain supernate, and freezing and drying to obtain a dry substance; and mixing the dry substance and the integripetal rhodiola herb and the bupleurum extract uniformly to obtain the compound integripetal rhodiola herb preparation. The compound integripetal rhodiola herb preparation has an antioxidation effect.

Methods of use and compositions useful for moisturizing skin, conditioning skin, reducing lines and wrinkles, evening skin tone, lightening skin tone, increasing skin radiance, elasticity, skin barrier function, and / or skin firmness, reducing photodamage, reducing sagging skin, increasing anti-oxidant capacity in skin, MMP-1, MMP-3, MMP-9, pro-inflammatory cytokines (e.g., lipoxygenase, IL-6, Il-8, TNF-alpha, or VEGF), and / or elastase expression in skin, and / or increasing collagen expression in skin, elastin expression in skin, laminin expression in skin, and / or fibronectin expression in skin are disclosed herein. The composition includes a combination of one or more of Rosmarinus officianalis leaf extract, Caulerpa lentillifera extract, Morus alba fruit extract, and Porphyridium cruentum extract.

The invention belongs to the technical field of processing of health-care products and relates to a compound mulberry preparation capable of improving superoxide dismutase activity. The preparation method for the compound mulberry preparation comprises the following steps of: extracting 12g of mulberry and 9g of medlar in ethanol of which the concentration is 70 percent and the amount is 10 timesthat of the mulberry and the medlar at room temperature for 10 hours, sealing and crushing the extract with ultrasonic waves for 30 minutes, filtering the extract, and extracting the filtration residue with ethanol of which the concentration is 70 percent and the amount is 6 times and 4 times that of the filtration residue again for one time respectively; combining the filtrates, reclaiming the ethanol to obtain an extract of the mulberry and medlar; centrifuging 20g of porphyridium cruentum with a centrifugal for 10 minutes, suspending the porphyridium cruentum in 50mmol / L of phosphate buffer of which the pH value is 7.4, crushing the porphyridium cruentum with ultrasonic waves, centrifuging the crushed porphyridium cruentum with a high-speed centrifugal for 15 minutes to obtain a liquidsupernatant, and refrigerating and drying the liquid supernatant to obtain a dried product; and uniformly mixing the dried product and the extract of the mulberry and medlar to obtain the mulberry preparation. The mulberry preparation can be applied to a natural antioxidant which is newly developed in fields, such as food, medicament and the like, and contributes to industrial production.