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Compound acanthopanax root preparation capable of improving activity of superoxide dismutase

A technology of superoxide and Acanthopanax, applied in medical preparations containing active ingredients, anti-toxins, food science, etc., can solve problems such as side effects

Inactive Publication Date: 2012-06-27
SHANDONG UNIV AT WEIHAI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Synthetic antioxidants have good antioxidant properties, but they have certain side effects

Method used

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  • Compound acanthopanax root preparation capable of improving activity of superoxide dismutase
  • Compound acanthopanax root preparation capable of improving activity of superoxide dismutase

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] 1, a kind of preparation method that can improve superoxide dismutase activity compound Acanthopanaxoid preparation, is characterized in that comprising the following steps:

[0020] (1) The weight ratio of raw materials is: Acanthopanax 9g, Polygala 6g, Porphyridium 10g;

[0021] (2) Add Acanthopanax and Polygala to 8 times the amount of 70% ethanol to extract 8 times at room temperature, then seal and sonicate for 30 minutes at a temperature of 30°C and a power of 80W, then filter with gauze, and use 6 times the amount and 4 times of the filter residue respectively. Multiple times of 70% ethanol was repeatedly extracted once, filtered to obtain the filtrate;

[0022] (3) The filtrate in (2) is combined, ethanol is reclaimed, and Acanthopanax and Polygala extract are obtained;

[0023] (4) Centrifuge Porphyridium algae with a 5000r / min centrifuge for 10min, then suspend it in pH 7.4, 50mmol / L phosphate buffer, perform ultrasonic crushing, break for 5s, interval 5s, 20...

Embodiment 2

[0027] 1, a kind of preparation method that can improve superoxide dismutase activity compound Acanthopanaxoid preparation, is characterized in that comprising the following steps:

[0028] (1) The weight ratio of raw materials is: Acanthopanax 12g, Polygala 9g, Porphyridum 20g;

[0029] (2) Add 10 times the amount of Acanthopanax and Polygala to 70% ethanol for leaching at room temperature for 10 hours, then seal and sonicate for 30 minutes at a temperature of 30°C and a power of 80W, then filter with gauze, and filter the residue with 6 times the amount and 4 Multiple times of 70% ethanol was repeatedly extracted once, filtered to obtain the filtrate;

[0030] (3) The filtrate in (2) is combined, ethanol is reclaimed, and Acanthopanax and Polygala extract are obtained;

[0031] (4) Centrifuge Porphyridium algae with a 5000r / min centrifuge for 10min, then suspend it in pH 7.4, 50mmol / L phosphate buffer, perform ultrasonic crushing, break for 5s, interval 5s, 20 times, use 10...

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Abstract

The invention belongs to the technical field of processing of health-care foods, and relates to a compound acanthopanax root preparation capable of improving activity of superoxide dismutase. A preparation method of the compound acanthopanax root preparation comprises the following steps of: adding 12g of acanthopanax root and 9g of thinleaf milkwort root-bark into 70 percent ethanol which is 10 times of the total amount of the acanthopanax root and the thinleaf milkwort root-bark for leaching at the room temperature for 10 hours, performing hermetic ultrasonic treatment for 30 minutes, filtering, and repeatedly extracting filter residues with two kinds of 70 percent ethanol, which are 6 times and 4 times of the total amount of the acanthopanax root and the thinleaf milkwort root-bark once respectively; combining filtrates and recovering ethanol to obtain acanthopanax root and thinleaf milkwort root-bark extracts; centrifuging 20g of porphyridium cruentum with a centrifugal machine for 15 minutes, suspending in 50 mmol / L of phosphoric acid buffer solution of which pH is 7.4, ultrasonically smashing, centrifuging by using a high-speed refrigerating centrifugal machine for 15 minutes to obtain a supernatant, and chilling and drying to obtain a dry substance; and uniformly mixing the dry substance with the acanthopanax root and thinleaf milkwort root-bark extracts to obtain the compound acanthopanax root preparation. The compound acanthopanax root preparation can be applied in the field of foods, medicaments and the like for developing a new natural antioxidant, and is beneficial to industrial production.

Description

technical field [0001] The invention belongs to the technical field of health food processing, and the invention relates to a compound Acanthopanax preparation capable of improving the activity of superoxide dismutase. The product of the invention can be used to develop new natural antioxidants in the fields of food, medicine and the like, and the product of the invention is beneficial to industrial production. Background technique [0002] Acanthopanax is the root and rhizome of Eleu-therococcus senticosus (Ruper.et Maxim.) Maxim. Spicy, slightly bitter, slightly warm in nature. Return spleen, kidney, liver channel, heart channel. Dispelling rheumatism, nourishing liver and kidney, strengthening muscles and bones, promoting blood circulation. Indications for anemofrigid-damp arthralgia, pain in the waist and knees, flaccidity of muscles and bones, delayed movement at the decimal point, physical weakness and weakness, bruises, fractures, edema, beriberi, dampness and itch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/69A61P39/06A23L1/29A23L33/00
Inventor 张崇禧侯欣池张成城郑友兰
Owner SHANDONG UNIV AT WEIHAI
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