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A method for harvesting Porphyridium algae based on foam flotation

A harvesting method and foam flotation technology, which is applied in the direction of single-cell algae and separation of microorganisms, can solve the problem of low recovery rate of Porphyridium coccus, and achieve the effects of easy industrialization, low input cost, and low addition amount

Active Publication Date: 2021-02-19
BAOJI UNIV OF ARTS & SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although the cells of Porphyridium purpurea and Pseudochloropsis eyelid belong to microalgae, they belong to different species of algae in terms of biological classification, so it cannot be considered that directly adding the same amount of rhamnolipid + aeration and air flotation conditions can achieve the same recovery rate as that of Pseudochloropsis eyelid, and the inventor found through experiments that the recovery rate of Porphyridium is not higher than 50% only by using rhamnolipid + inflatable air flotation

Method used

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  • A method for harvesting Porphyridium algae based on foam flotation
  • A method for harvesting Porphyridium algae based on foam flotation

Examples

Experimental program
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Effect test

Embodiment 1

[0029] A method for separation of Porphylococcus foam selection flotation, comprising the steps of:

[0030] (1) Pretreatment: the algae cell density of the porphyridium algae liquid to be harvested is 3.2638×10 7 cells / L, adjust the pH of the algae liquid of Porphyridum algae to be harvested to 7 by a pH regulator, and then add rhamnolipid to the algae algae to be harvested at a ratio of solid-to-liquid ratio (kg:L) of 0.05:1000 solution, protected from light at room temperature, and magnetically stirred at 800 rpm for 5 minutes to obtain the pretreated Porphyridium algae liquid;

[0031] (2) Flotation: transfer the pretreated Porphyrococcus algae liquid to a single-cell flotation machine, and then use terpineol oil at a solid-to-liquid ratio (solid-to-liquid ratio is kg: L, here refers to terpineol oil With the solid-to-liquid ratio of the algae liquid of Porphyridum algae to be harvested, the solid-to-liquid ratio of terpineol oil added in the following examples has the sa...

Embodiment 2

[0035] A method for separation of Porphylococcus foam selection flotation, comprising the steps of:

[0036] (1) Pretreatment: the algae cell density of the porphyridium algae liquid to be harvested is 3.2638×10 7cells / L, adjust the pH of the algae liquid of Porphyridum algae to be harvested to 9 through a pH regulator, and then add rhamnolipid to the algae algae to be harvested at a ratio of solid-to-liquid ratio (kg:L) of 0.09:1000 solution, at room temperature in the dark and magnetically stirred at a speed of 1200rpm for 15min to obtain the pretreated Porphyridium algae liquid;

[0037] (2) Flotation: Transfer the pretreated Porphyridium algae liquid to the single-tank flotation machine, then add pine oil at a ratio of solid-to-liquid ratio (kg:L) of 0.005:1000, and then set the aeration flow rate is 150L / h, the impeller speed is 800rpm, and the flotation stops after 10 minutes to obtain floating products and tailings wastewater;

[0038] (3) Post-treatment: use a scrape...

Embodiment 3

[0041] A method for separation of Porphylococcus foam selection flotation, comprising the steps of:

[0042] (1) Pretreatment: the algae cell density of the porphyridium algae liquid to be harvested is 3.2638×10 7 cells / L, adjust the pH of the algae liquid of Porphyridum algae to be harvested to 8 through a pH regulator, and then add rhamnolipid to the algae algae to be harvested at a ratio of solid-to-liquid ratio (kg:L) of 0.07:1000 solution, at room temperature, protected from light and magnetically stirred at a speed of 1000 rpm for 10 minutes, to obtain the pretreated Porphyridum algae liquid;

[0043] (2) Flotation: Transfer the pretreated Porphyridum algae liquid to the single-tank flotation machine, then add pinitol oil at a ratio of solid-liquid ratio (kg:L) 0.004:1000, and then set the aeration flow rate 100L / h, the impeller speed is 600rpm, and the flotation stops after 8 minutes to obtain floating products and tailings wastewater;

[0044] (3) Post-treatment: Use...

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Abstract

The invention discloses a method for harvesting porphyridium cruentum based on foam floatation. According to the method, rhamnoilpid is used in cooperation with a foaming agent, a floating product is collected in the mode of foam floatation, and finally the porphyridium cruentum is obtained. The method is suitable for treating porphyridium cruentum liquor within the range of 6.0*106 cells / L-2.0*109 cells / L as algal cell density in the porphyridium cruentum liquor, the concentration multiple of the algal cell density in the porphyridium cruentum liquor before treatment can reach 102 times, one or less hour is consumed for treatment, and the recovery rate of the porphyridium cruentum can reach 90% or above; floatation can be performed in a single-trough floating machine, in this way, investment cost is low, and industrialization is convenient.

Description

technical field [0001] The invention belongs to the field of microalgae biological harvesting, and in particular relates to a method for harvesting Porphyridium algae based on microalgae flotation. Background technique [0002] As the world's second largest economy, my country is facing the problems of shortage of traditional fossil energy and increasingly serious environmental pollution brought about by the rapid development of social economy, industrialization and urbanization. Energy microalgae is a renewable biomass energy. It has the advantages of wide distribution, high photosynthetic efficiency, short growth cycle, sufficient oil content, environmental friendliness, and does not occupy arable land resources. It is considered to be able to replace traditional fossil energy in the future. As a relatively common type of energy microalgae, Porphyridium is widely sourced (widely distributed in Taiyuan, Jinzhong, Anhui, Wuhan, Jingzhou, etc.), easy to cultivate (strong salt ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/12C12N1/02
CPCC12N1/02C12N1/12
Inventor 赵钺
Owner BAOJI UNIV OF ARTS & SCI
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