36 results about "Porphyridium sp." patented technology

The invention discloses a high-density and high-yield porphyridium continuous culture method. The method comprises the following steps: step 1, culturing porphyridium in a photobioreactor at high density to the middle and later periods of a logarithmic phase; step 2, continuously harvesting algae cell parts in high-density algae liquid; step 3, harvesting soluble exopolysaccharides in a supernate obtained by continuous harvesting, and supplementing the harvested supernate back to the photobioreactor; step 4, controlling the speed of feeding a culture medium concentrated solution into the photobioreactor; and repeating the steps 2-4 until the culture is ended. The method has the characteristics of high density, high yield and continuous production, can simultaneously harvest purple ball biomass, phycoerythrin, exopolysaccharide, polyunsaturated fatty acid and the like at high yield, realizes reuse of the culture solution, and improves the economical efficiency and environmental protection property of culture.

The invention discloses multiple-effect activation eye activating concentrate and a preparation method thereof. The multiple-effect activation eye activating concentrate comprises the following raw materials of a yeast fermentation product extract, acetyl hexapeptide-8, acetyl tetrapeptide-2, acetylated sodium hyaluronate, phoenix dactylifera seeds, a porphyridium cruentum extract, an emulsifyingagent, an emollient, a thickening agent and a preservative. The multiple-effect activation eye activating concentrate prepared by the preparation method disclosed by the invention has relatively strong antioxygenic property, can remove redundant free radicals in a body, activate skin cells, promote metabolism of the skin cells, reduce loss of collagen and elastin, improve black eyes, reduce eye wrinkles, improve eye skin compactness, improve skin roughness and resist aging, and is durable and stable in efficacy, and mild and non-irritant in action.

The invention discloses a composition containing porphyridium polypeptide and a preparation method of the composition. The composition comprises the following effective components in parts by weight:0.1-0.9 part of porphyridium polypeptide and 99.1 to 99.9 parts of a substrate. The preparation method comprises the following steps: an obtaining step of the porphyridium polypeptide: repeatedly freezing and thawing porphyridium, performing ultrasonic crushing, centrifuging an obtained porphyridium liquid, taking supernate, performing separating by vertical electrophoresis to obtain polypeptide with molecular weight of 5Kda or below, namely the porphyridium polypeptide; or taking the porphyridium, performing homogenizing, sterilizing, filtering and centrifuging, collecting supernatant, and performing ultrafiltration by using an ultrafiltration membrane system with a microporous membrane of 0.001-0.1 [mu]m to obtain the porphyridium polypeptide with the molecular weight cutoff 1KDa; and amixing step: mixing the porphyridium polypeptide with the matrix in parts by weight to obtain the composition containing the porphyridium polypeptide. The composition can promote wound healing, reducewound opening time and facilitate rehabilitation after body surface damage.

The invention discloses a method for simultaneously extracting multiple active substances in porphyridium, and belongs to the field of bioengineering. The method comprises the following steps: firstly, leaching porphyridium with cold water, separating a cold water leaching solution and porphyridium residue I, leaching the porphyridium residue I with ethanol or an ethanol aqueous solution, separating an ethanol or ethanol aqueous solution leaching solution and porphyridium residue II, leaching the porphyridium residue II with hot water, and separating a hot water leaching solution and porphyridium residue III, wherein the cold water leaching solution is a phycoerythrin extract product, the ethanol or ethanol aqueous solution leaching solution is a polyunsaturated fatty acid extract, and the hot water leaching solution is an intracellular polysaccharide extract. The extraction method is high in extraction efficiency, safe, simple to operate, non-toxic and beneficial to large-scale popularization and subsequent product development.

The invention discloses an alga-containing composition used for a cosmetic. The composition in dry weight contains the following components in percentage by mass: 0.003-0.125% of an alga mixture, 0.001-0.050% of radix ophiopogonis extract, 0.100-25.000% of betaine and the balance of deionized water, wherein the alga mixture is composed of porphyridium cruentum extract, bladderwrack extract and chondrus crispus extract, the content of the porphyridium cruentum extract is 0.001-0.005%, the content of the bladderwrack extract is 0.001-0.030%, and the content of the chondrus crispus extract is 0.001-0.090%. The invention also discloses a cosmetic composition applying the composition used for the cosmetic. The alga-containing composition used for the cosmetic has the effects of nature, high efficiency, easiness in absorption by virtue of a human body and long moisture preserving time and is applicable to manufacturing of various moisture preserving type skincare products.

The invention relates to the technical field of gene engineering, and particularly relates to a porphyridium chloroplast homologous recombination empty vector and application thereof. The vector comprises an upstream homologous arm, a downstream homologous arm, promoters and terminators, wherein a base sequence of the upstream homologous arm is shown in SEQ ID NO:1 of a porphyridium chloroplast gene group, a base sequence of the downstream homologous arm is shown in SEQ ID NO:2, base sequences of the promoters are shown in SEQ ID NO:3 and SEQ ID NO:4, base sequences of the terminators are shown in SEQ ID NO:5 and SEQ ID NO:6; and a corresponding base sequence which can form a multicistron structure with at least one exogenous gene and is shown in SEQ ID NO:7 is inserted between the corresponding promoter and the corresponding terminator. By adopting the porphyridium chloroplast homologous recombination empty vector disclosed by the invention, stable expression of multiple exogenous genes in the chloroplast can be realized.

The invention belongs to the technical field of processing of health-care foods, and relates to a compound acanthopanax root preparation capable of improving activity of superoxide dismutase. A preparation method of the compound acanthopanax root preparation comprises the following steps of: adding 12g of acanthopanax root and 9g of thinleaf milkwort root-bark into 70 percent ethanol which is 10 times of the total amount of the acanthopanax root and the thinleaf milkwort root-bark for leaching at the room temperature for 10 hours, performing hermetic ultrasonic treatment for 30 minutes, filtering, and repeatedly extracting filter residues with two kinds of 70 percent ethanol, which are 6 times and 4 times of the total amount of the acanthopanax root and the thinleaf milkwort root-bark once respectively; combining filtrates and recovering ethanol to obtain acanthopanax root and thinleaf milkwort root-bark extracts; centrifuging 20g of porphyridium cruentum with a centrifugal machine for 15 minutes, suspending in 50 mmol/L of phosphoric acid buffer solution of which pH is 7.4, ultrasonically smashing, centrifuging by using a high-speed refrigerating centrifugal machine for 15 minutes to obtain a supernatant, and chilling and drying to obtain a dry substance; and uniformly mixing the dry substance with the acanthopanax root and thinleaf milkwort root-bark extracts to obtain the compound acanthopanax root preparation. The compound acanthopanax root preparation can be applied in the field of foods, medicaments and the like for developing a new natural antioxidant, and is beneficial to industrial production.

The invention discloses a giant salamander feed. The giant salamander feed comprises the following components in parts by weight: 10 to 20 parts of crab meat, 20 to 25 parts of fishbone dust, 5 to 15parts of compound amino acids, 5 to 10 parts of plantain herb, 5 to 10 parts of verbena officinalis, 10 to 15 parts of Emblica officinalis pulp, 5 to 10 parts of porphyridium cruentum, 5 to 10 parts of laurate, 10 to 20 parts of soybean meal and 5 to 15 parts of complex vitamins. The invention also discloses a preparation method of the feed. The preparation method comprises the following steps: (1) treatment of Emblica officinalis; (2) preparation of compound amino acids; (3) preparation of complex vitamins; (4) pretreatment of raw materials; and (5) pelleting to obtain the giant salamander feed. The formula of the giant salamander feed is easy to obtain, the giant salamander feed is free of toxic action and is low in price, and the preparation method of the feed is simple, high in operability and low in preparation cost and is suitable for wide popularization and application; the physiological effects such as the immunity of the organism can be improved, the giant salamander are prevented from being infected by viruses, the appetite of the giant salamanders can be increased through fruit fragrance, and rapid growth of the giant salamanders is promoted.

The invention discloses a method for increasing the yield of ARA (arachidonic acid) produced through fermentation of Mortierella alpina by adding porphyridium. According to the method, porphyridium residues are added to a fermentation culture medium so as to increase the yield of the ARA. After the Mortierella alpina is cultured on a culture-medium-rich PDA (potato dextrose agar) plate, a conical seed culture flask is inoculated with spores for culture for 36 h-48 h, the fermentation culture medium is inoculated with a seed solution with the inoculation amount of 5%-10% for continuous fermentation culture, and the treated porphyridium residues are added during fermentation culture; tests prove that the method can obviously increase the yield of the ARA produced from the Mortierella alpine, has the advantages of simplicity in operation, capability of reducing the fermentation cost and reducing pollution treatment and the like and can be easily applied to the industry.

The invention discloses a method and a device for co-producing polysaccharide through high-density semi-continuous culture of porphyridium, and belongs to the technical field of marine microorganisms. The device comprises a cylindrical bioreactor with an open upper end and a closed lower end, the upper end is connected with a carbon dioxide inlet and an air inlet through a three-way pipe, a main pipeline of the three-way pipe is communicated to the bottom of the bioreactor, and gas flow controllers and valves are arranged on a carbon dioxide inlet branch pipe. A water inlet is further formed in the upper end of the bioreactor, a liquid outlet is formed in the bottom side wall of the bioreactor, and the LED lighting device is composed of a transparent glass cover and at least one LED lamp panel and extends into the bioreactor. The porphyridium is semi-continuously cultured on a large scale through the device, meanwhile, the porphyridium polysaccharide is co-produced by controlling the feeding condition, the renewal rate and the renewal period of the culture medium in the semi-continuous culture process, the yield of the porphyridium polysaccharide is greatly improved compared with that of batch culture, and the device is suitable for large-scale production.