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Lipase with fat degrading capability under acidic condition

A technology of acid lipase and lipase, applied in the fields of molecular biology, enzymology, and physiology, can solve problems such as the limitation of lipase resource development, the complexity of microbial growth conditions, etc., and achieve the effect of high acid tolerance

Inactive Publication Date: 2012-07-04
刘刚
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In view of the important industrial application value and huge potential demand of microbial lipase, many industrial laboratories and scientific research units have long been engaged in the tedious screening of high-yielding lipase microorganisms, but because of the complexity of microbial growth conditions, fats with special properties Enzyme resource development limited by microbial growth

Method used

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  • Lipase with fat degrading capability under acidic condition
  • Lipase with fat degrading capability under acidic condition
  • Lipase with fat degrading capability under acidic condition

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Obtaining of Acid Lipase Enzyme Solution

[0014] Synthesize the acid lipase gene according to SEQ NO.1, PCR amplify the synthesized product and plasmid pET28-a after digestion, ligate and transform E.coli BL21 (not limited to this plasmid and the corresponding recipient). 37. C liquid fermentation culture, after IPTG-induced expression, the cells are ultrasonically broken, and the supernatant is collected by centrifugation, which is the enzyme solution containing the acid lipase.

Embodiment 2

[0016] Acid-resistant lipase hydrolyzed oil

[0017] Add an appropriate amount of enzyme solution to 40% vegetable oil solution (pH3), and stir at 100 rpm for 2 hours. The oil phase contains fatty acids and the water phase contains glycerol as measured by chromatography, indicating that lipase can hydrolyze oil to produce glycerol and fatty acids .

[0018]

[0019]

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PUM

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Abstract

The invention discloses a nucleotide sequence and amino acid sequence of a lipase with fat degrading capability under acidic condition after modification, and is used for overcoming the defects that the enzyme activity rapidly decreases and the practicability is poor when the lipase is needed to be used under the acidic condition in the prior art, and realizing the advantages of high acid resistance, good stability, lowered cost, simplified process and good practicability of the lipase in the use process.

Description

technical field [0001] The invention belongs to the fields of molecular biology, enzymology and physiology. Specifically, it relates to an acid-resistant lipase DNA sequence and its corresponding amino acid sequence modified by means of molecular biology. Background technique [0002] Lipase (lipase, EC3.1.1.3) is a class of enzymes that catalyze the decomposition, synthesis and transesterification of triacylglycerides. The unique performance of the reaction has been widely used in food, oleochemical and other industries. With the broadening of application fields, it has been involved in emerging fields such as single-cell protein production, cosmetics production, environmental protection, new biomaterials, biosensors, biomedicine and bioenergy, and has broad application prospects. [0003] Lipase widely exists in animals, plants and microorganisms in nature. For example: 28 genera of bacteria, 4 genera of actinomycetes, 10 genera of yeast, and 23 genera of other fungi ca...

Claims

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Application Information

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IPC IPC(8): C12N9/16C12N15/55C12N15/63
Inventor 刘刚方井霞张柏松苏东风
Owner 刘刚
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