Method for breeding cytoplasmic male sterile material of brasica napus

Abstract

The invention belongs to the field of modern agriculture and rape breeding, and discloses a method for breeding a cytoplasmic male sterile material of brasica napus. The method comprises the following steps of: preparing white mustard and brasica napus somatic cell hybrids by electrofusion; backcrossing the obtained somatic cell hybrids continuously by using the brasica napus, and screening from backcrossed descendants to obtain sterile plants of which the chromosome number is 38; and performing the test cross on the sterile plants and a brasica napus strain, screening to obtain a maintainer line, and backcrossing continuously by utilizing the maintainer line to breed a brasica napus sterile line.

Description

technical field [0001] The electrofusion method is used to realize the recombination of the cytoplasm of the hybrid parents, combined with conventional hybridization to obtain a cytoplasmic male sterile material of Brassica napus, which can be used in the fields of modern agriculture and rape breeding. Background technique [0002] Cytoplasmic male sterility (CMS) is a maternally inherited phenomenon and an important way to utilize heterosis in plants, and the utilization of heterosis is an important means to increase the yield of rapeseed. At present, about 70% of the hybrids used in my country's rapeseed production are formulated with cytoplasmic male sterility as the pollination control system. [0003] At present, the cytoplasmic male sterile lines of rape in my country are mainly Pol CMS and Shan2A CMS, and there are mainly the following main problems in production: ① The fertility of PolCMS and Shan 2A CMS is unstable and sensitive to low temperature. A small amount of...

AI Technical Summary

Problems solved by technology

[0003] At present, the cytoplasmic male sterile lines of rape in my country are mainly Pol CMS and Shan2A CMS, and there are mainly the following main problems in production: ① The fertility of PolCMS and Shan 2A CMS is unstable and sensitive to low temperature. A small amount of pollen will cause self-fertilization, so the purity of hybrids is difficult to guarantee, and there is a greater risk in production

② The type of cytoplasm is relatively single. PolCMS and Shan 2A CMS male sterile lines are mainly used in the production of hybrid rapeseed in my country. These two male sterile lines are basically of the same type, which increases the production risk caused by a single cytoplasm

③ Due to the limitation of patent rights, some foreign excellent CMS lines are difficult to be directly applied in China

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