Ion chromatograph and chemoluminescence detection method for determining catecholamine substances

A chemiluminescence detection and catecholamine technology, which is applied in measuring devices, scientific instruments, material separation, etc., can solve the problems of low detection sensitivity, error of measurement results, poor reproducibility, etc., and achieve improved sensitivity, low detection limit, and luminous efficiency Improved effect

Inactive Publication Date: 2014-06-04
ZHEJIANG UNIV
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Problems solved by technology

At present, the common method for the determination of catecholamines is mainly high-performance liquid chromatography-fluorescence detection method, which has low detection sensitivity and is limited for some low-content samples.
In addition, electrochemical methods are also used for detection, but their reproducibility is poor, resulting in large errors in the measurement results.

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  • Ion chromatograph and chemoluminescence detection method for determining catecholamine substances
  • Ion chromatograph and chemoluminescence detection method for determining catecholamine substances
  • Ion chromatograph and chemoluminescence detection method for determining catecholamine substances

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Embodiment Construction

[0018] Below in conjunction with embodiment technical scheme of the present invention is described in further detail:

[0019] Determination of catecholamines in urine by ion chromatography-chemiluminescence detection:

[0020] Specific steps are as follows:

[0021] 1. Preparation of standard solution: Accurately weigh 10 mg each of epinephrine, norepinephrine, and dopamine standard samples, place them in three 10ml volumetric flasks respectively, add 50% acetonitrile, shake to dissolve, and set the volume to the mark;

[0022] 2. Preparation of standard curve: prepare standard solutions with different concentrations by step-by-step dilution;

[0023] 3. Sample pretreatment: Take an appropriate amount of urine from healthy volunteers, centrifuge at 10,000 rpm for 10 minutes, take the supernatant and dilute it by a certain number of times, then filter it with a 0.45 μm filter membrane and inject it for determination;

[0024] 4. Selection of eluent: test the proportion of e...

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Abstract

The invention provides a novel detection method for catecholamine substances, which connecting in series an ion chromatograph and a chemoluminescence detector, wherein the required leacheate is separated as per ion chromatography, a suitable chemoluminescence system is selected, the to-be-detected substance is formed into ions in the leacheate and separated through the ion chromatography, so as to be detected by the chemoluminescence detector. The method avoids adopting organic solvents as methanol to serve as the leacheate, enhances the luminescence efficiency, greatly improves the sensitivity of determination, and is applicable to detecting the catecholamine substances in the environment and the samples in food and biomedicine, and can be further applied to the research of the pharmacokinetics.

Description

technical field [0001] The invention provides a method for determining catecholamines, specifically, a new method for determining catecholamines by ion chromatography-chemiluminescence detection. Background technique [0002] Catecholamine is a neurological substance containing catechol and amine groups, which has important physiological functions in the human body. Common catecholamines mainly include epinephrine, norepinephrine, and dopamine, etc., and their content changes in body fluids can show different pathological conditions, so their accurate determination is of great clinical significance. At present, the common method for the determination of catecholamines is mainly high-performance liquid chromatography-fluorescence detection method. The detection sensitivity of this method is not high, and the determination is limited for some low-content samples. In addition, electrochemical methods are also used for detection, but their reproducibility is poor, resulting in ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
Inventor 吴宏伟朱岩陈梅兰
Owner ZHEJIANG UNIV
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