Oriental lily seed ball virus removing method

Abstract

The invention provides an oriental lily seed ball virus removing method, which specifically comprises the following steps of: A) stripping of explant growth points, B) heat treatment virus removal, C) cleaning and sterilization of explants, D) acquisition, induction and culture of growth points, E) virus-free culture and F) virus detection. The oriental lily seed ball virus removing method has the advantages that the defects existing in the prior art are overcome, the defects caused by a reason that a single method is used are overcome by comprehensively using a heat treatment virus removing method, a shoot tip culture virus removing method and a callus virus removing method, the probability of virus cross infection can be effectively reduced, the virus removal rate is high, the survival rate is high and the culture period of seed balls can be effectively shortened.

Description

technical field

[0001] The invention belongs to a method for testing or treating seeds before sowing or planting, in particular to a method for detoxifying lily bulbs. Background technique

[0002] Oriental lily is the largest and most beautiful category of lily hybrids. It can emit a pleasant fragrance, so it is also called perfume lily. It is deeply loved by people and is a large category of lily widely cultivated in the world. hybrid line. Lilies mainly reproduce by cuttings and balls, but long-term asexual reproduction causes viruses to accumulate in the body, causing various viral diseases, and seriously affecting the quality of bulbs and cut flowers. In recent years, with the number of lily introductions in my country and With the rapid increase of the planting area and the irregular self-propagation of the bulbs, viral diseases began to be prevalent in various lily planting areas in my country. The general incidence of viral diseases is 40-50%, and the second-generati...

Examples

Embodiment 1

[0025] Example 1: The detoxification method for oriental lily bulbs comprises the following steps:

[0026] A, stripping of explant growth point: select high-quality bulbs, soak for 30 minutes with a 20% carbendazim 600-fold solution; mix the sterilized peat with water so that its water content is 45%, Standby; then plant the bulbs soaked in carbendazim in the wet peat, plant 20 bulbs in each box, pour root water, and then sprinkle the carbendazim solution soaked in the bulbs on the surface; press The planting method of cut flowers is managed normally, and the water content of peat is kept at 45%. Under the conditions of temperature at 21°C, humidity at 70%, light intensity at 1500Lx, and light time at 8h / d, after 5 to 7 days, heat treatment is carried out when the buds grow to 5cm. Detox.

[0027] B. Detoxification by heat treatment: The temperature is controlled at 35°C, the humidity is 70%, the light intensity is 1500Lx, and the light time is 8h / d. After 7-15 days of cu...

Embodiment 2

[0032] Example 2: The detoxification method for oriental lily bulbs comprises the following steps:

[0033] A, stripping of explant growth point: select high-quality bulbs, soak 30 minutes in 600 times solution of carbendazim with concentration of 30%; mix the sterilized peat with water to make its water content 50%, Standby; then plant the bulbs soaked in carbendazim in the wet peat, plant 22 bulbs in each box, pour root water, and then sprinkle the carbendazim solution soaked in the bulbs on the surface; press The planting method of cut flowers is managed normally, and the moisture of peat is kept at 50%. Under the conditions of temperature at 22°C, humidity at 75%, light intensity of 1800Lx, and light time of 10h / d, after 5 to 7 days, the buds grow to 5.5cm. Detoxification by heat treatment.

[0034] B. Detoxification by heat treatment: The temperature is controlled at 36°C, the humidity is 80%, the light intensity is 1500Lx, and the light time is 10h / d. After 7-15 days ...

Embodiment 3

[0040] Embodiment 3: The oriental lily bulb detoxification method comprises the following steps:

[0041] A, stripping of explant growth point: choose high-quality bulbs, soak for 30 minutes with a 600-fold solution of carbendazim with a concentration of 40%; mix the sterilized peat with water to make its water content 55%, Standby; then plant the bulbs soaked in carbendazim in the wet peat, plant 25 bulbs in each box, pour root water, and then sprinkle the carbendazim solution soaked in the bulbs on the surface; press The planting method of cut flowers is managed normally, and the moisture content of peat is kept at 55%. Under the conditions of temperature at 23°C, humidity at 80%, light intensity of 2000Lx, and light time of 12h / d, after 5 to 7 days, heat treatment is carried out when the buds grow to 6cm. Detox.

[0042] B. Detoxification by heat treatment: The temperature is controlled at 38°C, the humidity is 85%, the light intensity is 1500Lx, and the light time is 1...

technical field

[0001] The invention belongs to a method for testing or treating seeds before sowing or planting, in particular to a method for detoxifying lily bulbs. Background technique

[0002] Oriental lily is the largest and most beautiful category of lily hybrids. It can emit a pleasant fragrance, so it is also called perfume lily. It is deeply loved by people and is a large category of lily widely cultivated in the world. hybrid line. Lilies mainly reproduce by cuttings and balls, but long-term asexual reproduction causes viruses to accumulate in the body, causing various viral diseases, and seriously affecting the quality of bulbs and cut flowers. In recent years, with the number of lily introductions in my country and With the rapid increase of the planting area and the irregular self-propagation of the bulbs, viral diseases began to be prevalent in various lily planting areas in my country. The general incidence of viral diseases is 40-50%, and the second-generati...

Examples

Embodiment 1

[0025] Example 1: The detoxification method for oriental lily bulbs comprises the following steps:

[0026] A, stripping of explant growth point: select high-quality bulbs, soak for 30 minutes with a 20% carbendazim 600-fold solution; mix the sterilized peat with water so that its water content is 45%, Standby; then plant the bulbs soaked in carbendazim in the wet peat, plant 20 bulbs in each box, pour root water, and then sprinkle the carbendazim solution soaked in the bulbs on the surface; press The planting method of cut flowers is managed normally, and the water content of peat is kept at 45%. Under the conditions of temperature at 21°C, humidity at 70%, light intensity at 1500Lx, and light time at 8h / d, after 5 to 7 days, heat treatment is carried out when the buds grow to 5cm. Detox.

[0027] B. Detoxification by heat treatment: The temperature is controlled at 35°C, the humidity is 70%, the light intensity is 1500Lx, and the light time is 8h / d. After 7-15 days of cu...

Embodiment 2

[0032] Example 2: The detoxification method for oriental lily bulbs comprises the following steps:

[0033] A, stripping of explant growth point: select high-quality bulbs, soak 30 minutes in 600 times solution of carbendazim with concentration of 30%; mix the sterilized peat with water to make its water content 50%, Standby; then plant the bulbs soaked in carbendazim in the wet peat, plant 22 bulbs in each box, pour root water, and then sprinkle the carbendazim solution soaked in the bulbs on the surface; press The planting method of cut flowers is managed normally, and the moisture of peat is kept at 50%. Under the conditions of temperature at 22°C, humidity at 75%, light intensity of 1800Lx, and light time of 10h / d, after 5 to 7 days, the buds grow to 5.5cm. Detoxification by heat treatment.

[0034] B. Detoxification by heat treatment: The temperature is controlled at 36°C, the humidity is 80%, the light intensity is 1500Lx, and the light time is 10h / d. After 7-15 days ...

Embodiment 3

[0040] Embodiment 3: The oriental lily bulb detoxification method comprises the following steps:

[0041] A, stripping of explant growth point: choose high-quality bulbs, soak for 30 minutes with a 600-fold solution of carbendazim with a concentration of 40%; mix the sterilized peat with water to make its water content 55%, Standby; then plant the bulbs soaked in carbendazim in the wet peat, plant 25 bulbs in each box, pour root water, and then sprinkle the carbendazim solution soaked in the bulbs on the surface; press The planting method of cut flowers is managed normally, and the moisture content of peat is kept at 55%. Under the conditions of temperature at 23°C, humidity at 80%, light intensity of 2000Lx, and light time of 12h / d, after 5 to 7 days, heat treatment is carried out when the buds grow to 6cm. Detox.

[0042] B. Detoxification by heat treatment: The temperature is controlled at 38°C, the humidity is 85%, the light intensity is 1500Lx, and the light time is 1...