Medicament applicable to intervention shrimp ovary ripening and preparation method as well as application thereof
An interventional ovarian technology, applied in application, drug combination, climate change adaptation, etc., can solve the problems of non-reusable use, decline in offspring quality, high mortality, etc., to promote synchronous development and maturation of ovary, stable source, and method simple and easy effects
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[0025] Example 1: Ripening of Procambarus clarkii ovaries at the non-yolk stage
[0026] A. Drug preparation:
[0027] 1. Peel 20g of dried bletilla striata, cut into small pieces of 1.2mm, add 20 times (V / M) three-distilled water, soak at 4°C for 36 hours, put it in a water bath at 70°C for 4-8 hours, and Filter through a 1mm filter, place the filtrate at 4°C, add 5 times (V / M) triple distilled water to the filter residue and place in a 70°C water bath for 2 hours, repeat the decoction 3 times, filter with a 1mm filter, and combine the filtrates.
[0028] 2. Vibrate the above-mentioned filtrate with ultrasonic waves (ultrasonic conditions are as follows: Φ25mm fixed probe, working time 10s, gap 20s, amplitude 30um, container placed in ice-water mixture) for 30 minutes, then centrifuge at 6000g for 20 minutes, collect the supernatant, and check Whether the supernatant can pass through a 30G syringe needle.
[0029] 3. Add 10 times the volume of absolute ethanol to the supern...
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