Medicament applicable to intervention shrimp ovary ripening and preparation method as well as application thereof

An interventional ovarian technology, applied in application, drug combination, climate change adaptation, etc., can solve the problems of non-reusable use, decline in offspring quality, high mortality, etc., to promote synchronous development and maturation of ovary, stable source, and method simple and easy effects

Inactive Publication Date: 2013-06-19
HUAZHONG AGRI UNIV
View PDF4 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

On the one hand, removing the eyestalk has a significant effect on ripening, but on the other hand, it cannot avoid the following negative effects: (1) There is a high rate of parental death after removing the eyestalk (even by ligation or pinching the base of the eyestalk) Individuals that survive and lay eggs by chance will also have permanent damage, and some precious parental resources cannot be reused (2) After removing the eyestalk, the gonads continue to develop, and the quality of the eggs and hatched offspring is significantly reduced (Laufer et al. 1999)

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Medicament applicable to intervention shrimp ovary ripening and preparation method as well as application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Ripening of Procambarus clarkii ovaries at the non-yolk stage

[0026] A. Drug preparation:

[0027] 1. Peel 20g of dried bletilla striata, cut into small pieces of 1.2mm, add 20 times (V / M) three-distilled water, soak at 4°C for 36 hours, put it in a water bath at 70°C for 4-8 hours, and Filter through a 1mm filter, place the filtrate at 4°C, add 5 times (V / M) triple distilled water to the filter residue and place in a 70°C water bath for 2 hours, repeat the decoction 3 times, filter with a 1mm filter, and combine the filtrates.

[0028] 2. Vibrate the above-mentioned filtrate with ultrasonic waves (ultrasonic conditions are as follows: Φ25mm fixed probe, working time 10s, gap 20s, amplitude 30um, container placed in ice-water mixture) for 30 minutes, then centrifuge at 6000g for 20 minutes, collect the supernatant, and check Whether the supernatant can pass through a 30G syringe needle.

[0029] 3. Add 10 times the volume of absolute ethanol to the supern...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention belongs to the technical field of aquaculture, and particularly relates to a medicament applicable to intervention shrimp ovary ripening and a preparation method as well as application thereof. The preparation method is characterized by comprising the following three steps of: extracting bletilla striata polysaccharide as a carrier; preparing bletilla striata polysaccharide sol of ashrimp blood lymph characteristic; and preparing a ripening intervention medicament. Experiments show that the brooding rate is not less than that of an eyestalk ablation group when the medicament isapplied to red swamp crayfishes at the early stage of vitellogenesis under the same breeding condition. The death rate tends to be zero after the shrimps are treated for a week. Comparatively, the death rate for bilateral eyestalk ablation under the same condition in a week is 71.67 percent, and the death rate for a unilateral eyestalk ablation group under the same condition in a week is 57.5 percent. After the medicament is used for treating the red swamp crayfishs during vitellogenesis for 45 days, the brooding rate is 84.82 percent, the death rate in a week is only 1.67 percent, the stability is guaranteed and the death rate is obviously reduced, P is less than 0.01, and synchronous development and maturing of ovary are promoted. The medicament has a stable material source; and the method is simple and practical and has the prospect of being applied as an alternative method for an eyestalk ablation shrimp ovary ripening method.

Description

technical field [0001] The invention belongs to the technical field of aquaculture, and in particular relates to a medicine suitable for interventional ripening of shrimp ovaries, a preparation method and application thereof. The intervention agent of the invention is used to release the neuroendocrine inhibitory effect of eye stalk on the ovarian development process at a certain period of time, so as to achieve the purpose of promoting better development of shrimp ovaries and artificial ripening. Background technique [0002] The method commonly used in production practice to induce ovarian development and sexual maturity of economical crustaceans is to remove eyestalk and its derivatives. These techniques include: (1) removal of the eye stalk; (2) cauterization of the eye stalk; (3) ligation or pinching of the base of the eye stalk (T. subramoniam, 1999). On the one hand, removing the eyestalk has a significant effect on ripening, but on the other hand, it cannot avoid th...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Patents(China)
IPC IPC(8): A61K31/715A61K31/454A61P15/08A01K61/00
CPCY02A40/81
Inventor 刘胜利龚世园黄文虎李金梅孙瑞杰袁汉文李强林郁葱闫建林袁兆嘉龚文杰
Owner HUAZHONG AGRI UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products