Human oocyte in-vitro pre-fertilization culture method, series of culture solutions and application

A technology of in vitro maturation culture and culture medium, which is applied in the field of cell endocrine, can solve problems such as not being the best environment for eggs, and achieve the effects of improving egg quality, clinical pregnancy rate, and normal fertilization rate

Pending Publication Date: 2022-03-08
艾尔斯(浙江)医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the above-mentioned methods in the prior art exist: after egg retrieval and before fertilization, the eggs are in the "fertilization medium", although there are only a few hours, this fertilization medium is not the best environment for the eggs

Method used

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  • Human oocyte in-vitro pre-fertilization culture method, series of culture solutions and application
  • Human oocyte in-vitro pre-fertilization culture method, series of culture solutions and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] Embodiment 1. Human oocyte in vitro fertilization system: the method of using egg in vitro maturation culture fluid, fertilization culture fluid and embryo development culture fluid in combination

[0064] 1. A series of culture medium for culturing human oocytes before in vitro fertilization and its application

[0065] (1) egg in vitro maturation culture medium, its composition comprises:

[0066] Liquid A: including 7g / L sodium chloride, 0.5g / L potassium chloride, 0.04g / L magnesium sulfate heptahydrate, 0.05g / L sodium dihydrogen phosphate, 3g / L sodium bicarbonate, 2g / L LD-(+ )-glucose, 0.3g / L sodium pyruvate, 0.3g / L calcium chloride dihydrate, 2.2g / L DL-sodium lactate, 0.2g / L alanyl glutamine, 0.02g / L L-tianmen Paragine, 0.02g / L L-aspartic acid, 0.01g / L glycine, 0.02g / L L-proline, 0.03g / L L-serine, 0.3g / L L-hydrochloride amino acid, 0.035g / L L-cystine hydrochloride, 0.035g / L L-histidine hydrochloride monohydrate, 0.1g / L L-isoleucine, 0.1g / L L -Leucine, 0.15g / L lys...

Embodiment 2

[0076] Example 2, human oocyte in vitro fertilization system: the method of using egg in vitro maturation culture fluid, fertilization culture fluid and embryo development culture fluid in combination

[0077] 1. A series of culture medium for culturing human oocytes before in vitro fertilization

[0078] (1) egg in vitro maturation culture medium, its composition comprises:

[0079] A liquid: including 5.5g / L sodium chloride, 0.25g / L potassium chloride, 0.02g / L magnesium sulfate heptahydrate, 0.03g / L sodium dihydrogen phosphate, 2.1g / L sodium bicarbonate, 1g / L- (+)-glucose, 0.15g / L sodium pyruvate, 0.15g / L calcium chloride dihydrate, 1.5g / L DL-sodium lactate, 0.1g / L alanyl glutamine, 0.01g / L L -Asparagine, 0.01g / L L-aspartic acid, 0.006g / L glycine, 0.01g / L L-proline, 0.01g / L L-serine, 0.1g / L L- Arginine hydrochloride, 0.02g / L L-cystine hydrochloride, 0.02g / L L-histidine hydrochloride monohydrate, 0.05g / L L-isoleucine, 0.05g / L L-leucine, 0.07g / L lysine hydrochloride, 0.01g / ...

Embodiment 3

[0089] Example 3, human oocyte in vitro fertilization system: the method of using egg in vitro maturation culture fluid, fertilization culture fluid and embryo development culture fluid in combination

[0090] 1. A series of culture medium for culturing human oocytes before in vitro fertilization

[0091] (1) egg in vitro maturation culture medium, its composition comprises:

[0092]A liquid: including 8.5g / L sodium chloride, 0.7g / L potassium chloride, 0.06g / L magnesium sulfate heptahydrate, 0.08g / L sodium dihydrogen phosphate, 5g / L sodium bicarbonate, 3g / LD-( +)-glucose, 0.5g / L sodium pyruvate, 0.5g / L calcium chloride dihydrate, 3g / L DL-sodium lactate, 0.3g / L alanyl glutamine, 0.04g / L L-tianmen Paragine, 0.03g / L L-aspartic acid, 0.02g / L glycine, 0.04g / L L-proline, 0.05g / L L-serine, 0.4g / L L-hydrochloride amino acid, 0.05g / L L-cystine hydrochloride, 0.05g / L L-histidine hydrochloride monohydrate, 0.2g / L L-isoleucine, 0.2g / L L - Leucine, 0.3g / L lysine hydrochloride, 0.05g / L L...

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Abstract

The invention discloses a human oocyte in-vitro pre-fertilization culture method, a series of culture solutions and application, and the human oocyte in-vitro pre-fertilization culture method after ovum obtaining comprises the following steps: pre-culturing obtained cumulus cell-ovum-complex (COC) in an ovum in-vitro maturation culture solution for 3-6 hours, after the eggs are obtained and before in-vitro fertilization, synchronous maturation of cell nucleuses and cytoplasm of the eggs can be further promoted by culturing for 3-6 hours, the quality of the eggs is improved, and then the normal fertilization rate, the high-quality cleavage stage embryonic development rate, the blastocyst formation rate and the high-quality blastocyst rate can be improved by cooperatively using the fertilization culture solution and the embryonic development culture solution.

Description

technical field [0001] The embodiments of the present invention relate to the field of cellular endocrine technology, and in particular to a method for culturing human oocytes before in vitro fertilization, a series of culture solutions and applications. Background technique [0002] Follicular granulosa cells (Granulosa Cells, GCs) are somatic cells in the follicle, which play a vital role in the growth and development of the follicle. As the follicle develops to the antral follicular stage, granulosa cells differentiate into two spatially and functionally distinct populations: cumulus cells (CCs) that surround the oocyte and wall granules that line the follicle wall Cells (Mural Granulosa Cells, MGCs). It is generally believed that cumulus cells and parietal granulosa cells are the same cell with the same origin but different positions. Because according to different ovulation stimulation schemes and individual ovarian responses, the size of all follicles is not uniform,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/073C12N5/075
CPCC12N5/0609C12N5/0603C12N2500/05C12N2500/32C12N2500/38C12N2500/35C12N2500/30
Inventor 千日成
Owner 艾尔斯(浙江)医学科技有限公司
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