Preparation method of acellular dermal matrix dressing

A decellularized dermis, decellularized technology, applied in the fields of medical science, bandages, absorbent pads, etc., can solve the problems of long production cycle, matrix damage, complicated operation, etc., and achieve the effect of short degreasing time, short time, and complete decellularization.

Active Publication Date: 2012-07-18
江苏优创生物医学科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The existing acellular dermal matrix materials have the following disadvantages: the operation is more complicated, the production cycle is longer, and the efficiency is lower; it is easy to cause damage to the matrix

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] A method for preparing an acellular dermal matrix dressing of the present invention, the product is prepared from pigskin, cowhide or sheepskin, comprising the following steps:

[0016] a. Process for preparing split skin: using a skin remover to make animal skin into a split skin with a thickness of 0.1 mm, and using a punch to make the split skin into a mesh-shaped split skin;

[0017] b. Virus inactivation process: The virus inactivation solution composed of peracetic acid with a mass concentration of 0.1% and sodium chloride with a mass concentration of 1.0% was used to inactivate the tomographic skin for 30 minutes. The ratio is 1: 4;

[0018] c. Degreasing process: use a mixed degreasing agent with a mass concentration of 1.0% soda ash, a mass concentration of 1.0% caustic soda and a mass concentration of 0.1% Pingpingjia for degreasing for 30 minutes, and the mass ratio of the skin sheet to the mixed degreasing agent is 1: 4;

[0019] d. Decellularization proces...

Embodiment 2

[0023] A method for preparing an acellular dermal matrix dressing of the present invention, the product is prepared from pigskin, cowhide or sheepskin, comprising the following steps:

[0024] a. Process for preparing split skin: using a skin remover to make animal skin into a split skin with a thickness of 0.1 mm, and using a punch to make the split skin into a mesh-shaped split skin;

[0025] b. Virus inactivation process: The virus inactivation solution composed of peracetic acid with a mass concentration of 0.1% and sodium chloride with a mass concentration of 1.0% was used to inactivate the tomographic skin for 30 minutes. The ratio is 1: 4;

[0026] c. Degreasing process: use a mixed degreasing agent with a mass concentration of 1.0% soda ash, a mass concentration of 1.0% caustic soda and a mass concentration of 0.1% Pingpingjia for degreasing for 30 minutes, and the mass ratio of the skin sheet to the mixed degreasing agent is 1: 4;

[0027] d. Decellularization proces...

Embodiment 3

[0031] A method for preparing an acellular dermal matrix dressing, the product is prepared from pigskin, cowhide or sheepskin, comprising the following steps:

[0032] a. Process for preparing split skin: using a skin remover to make animal skin into a split skin with a thickness of 1.0mm, and using a puncher to make the split skin into a mesh-shaped split skin;

[0033] B. Virus inactivation process: the virus inactivation solution composed of peracetic acid with a mass concentration of 1.0% and sodium chloride with a mass concentration of 3.0% was used to inactivate the tomographic skin for 45 minutes, the quality of the skin and the virus inactivation solution The ratio is 1:5.5;

[0034] C, degreasing process: the mixed degreasing agent that adopts mass concentration 2.5% soda ash, mass concentration 2.5% caustic soda and mass concentration 0.5% sodium dodecylalanine to degrease for 45 minutes, the mass ratio of skin sheet and mixed degreasing agent is 1:5.5;

[0035] d....

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Abstract

The invention discloses a preparation method of an acellular dermal matrix dressing. The acellular dermal matrix is prepared from animal skin by segmented graft preparing, viruses inactivating, degreasing, decellularizing, iodizing, lyophilizing, packaging and radiation disinfecting. The method has the advantages of short degreasing time, high efficiency, complete decellurarization, light damage to natural structure of extracellular matrix of hypodermal cell, and antibacterial function.

Description

technical field [0001] The invention relates to a method for preparing an acellular dermal matrix dressing. Background technique [0002] The existing acellular dermal matrix materials have the following disadvantages: the operation is complicated, the production cycle is long, and the efficiency is low; the matrix is ​​easily damaged. Therefore, it is necessary to provide a new technical solution to solve the above problems. Contents of the invention [0003] The purpose of the present invention is to provide a method for preparing an acellular dermal matrix dressing with short production cycle, high efficiency and light damage to the natural structure of dermal extracellular matrix. [0004] The technical scheme adopted in the present invention is: [0005] A method for preparing an acellular dermal matrix dressing, the product is prepared from pigskin, cowhide or sheepskin, comprising the following steps: [0006] a. Process for preparing split skin: using a skin rem...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L15/40A61L15/44
Inventor 潘银根潘华倩
Owner 江苏优创生物医学科技有限公司
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