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Method for identifying male silk of mulberry silkworm

A technology for silk and mulberry silk, which is applied in the field of judging male mulberry silk, can solve the problem that there is no one identifying male silk, and achieves the effect of simple and convenient method and accurate result.

Inactive Publication Date: 2012-07-18
SUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, the existing technology can only realize the identification of silkworm cocoons, how to determine silkworm silk, there is no method for identifying male silkworm silk at present

Method used

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  • Method for identifying male silk of mulberry silkworm
  • Method for identifying male silk of mulberry silkworm
  • Method for identifying male silk of mulberry silkworm

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0015] In this example, a number of silkworm cocoons of the silkworm variety "101×102" with fluorescent cocoon color identification were taken, and a fluorescent detector was used to distinguish the female cocoons with purple fluorescent color and the male cocoons with light yellow fluorescent color. Dry, divide and prepare.

[0016] Take 25g of silkworm cocoons, cut them into 10mm*10mm pieces with scissors, add 500ml of 0.5% sodium carbonate solution to a 1000ml beaker, boil for 45min, remove the cocoons, rinse them with tap water, put them in a 1000ml beaker, add 0.5% Neutral soap 500ml, degumming at 100°C for 30 minutes, rinse with water, and then rinse with deionized water once. Dry in an oven at 50°C to obtain degummed silk fibroin. Put the degummed silk fibroin into 250ml of calcium chloride solution with a concentration of 50%, heat it to boiling with a 500ml beaker, and stir to fully dissolve the silk fibroin. After the dissolution is complete, cool and filter, put t...

Embodiment 2

[0026] In the present embodiment, get 1 female cocoon shell and 9 male cocoon shells The cocoon silk fibroin samples prepared by mixing were tested for amino acid components. The compositions of tyrosine, methionine and aspartic acid in the silk fibroin are shown in Table 3.

[0027] table 3

[0028]

[0029] See Table 3, under the condition of significance level α=0.10, make X for the data 2 Test Analysis, X 2 =5.25*, greater than 4.61, reaching the level of significant difference.

[0030] This example proves that the composition ratio of the three amino acids tyrosine, methionine and aspartic acid between the silk fibroin of the mixed cocoon and the silk fibroin of the male cocoon composed of 10% female cocoon and 90% male cocoon, Under the condition of significant level α=0.10, through its X 2 Value compared with 4.61, can accurately determine whether the detected silk is true or false.

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Abstract

The invention discloses a method for identifying male silk of mulberry silkworm, and belongs to the technical field of silk production of textile engineering. The method comprises the following steps of: degumming tested silk to obtain pure silk fibroin and preparing into a powdery sample; testing content values of three amino acids, i.e., tyrosine, methionine and aspartic acid, in the sample in the unit of milligram / milliliter; obtaining chi-square X2 by using a chi-square test method based on the content values of the three amino acids in the silk fibroin of the standard male silk; and judging the tested silk as the male silk if X2 is less than 4.61 under the condition of significance level alpha being equal to 0.10. The content values of the three amino acids in the silk fibroin of the silk are used as a judgment basis; the chi-square test is adopted for processing; and the method is simple and convenient, has an accurate result and has a positive effect on promotion of high-grade male silk and products thereof on domestic and foreign markets.

Description

technical field [0001] The invention relates to a method for discriminating silkworm male silk, which belongs to the technical field of silk making in textile engineering. Background technique [0002] In silk production, compared with the female silk, the male cocoon silk has the characteristics of fine cocoon silk fineness, excellent clarity, small single cocoon fineness difference, good cohesion force, and high-grade raw silk. The fabric has good elasticity, and the silk surface is flat, crisp, smooth and waxy, and feels thick. At present, male silk with 6A grade 13 / 15D specification is used for processing weaving medical sutures, and 6A grade 20 / 22D male silk is used for processing high-end wedding dresses and silk fabrics. The application of male silk in extended products shows its uniqueness. It has the advantage of high grade. The key to preparing male silk is to distinguish male and female cocoons. CN1515150 discloses a method for judging the sex of silkworm cocoon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/36
Inventor 虞晓华
Owner SUZHOU UNIV
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