Method for culturing golden larch callus by taking phyllopodium as explant

A technology of callus and culture method, which is applied in the field of tissue culture of pine plants, can solve problems such as the shortage of pine chinensis medicinal resources, achieve the effects of solving the shortage of plant resources and medicinal material resources, reducing production costs, and shortening the growth cycle

Inactive Publication Date: 2012-07-25
CHENGDU UNIV
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  • Summary
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Problems solved by technology

[0007] The object of the present invention is to provide a method for culturing callus of Pine chinensis using leaf primordia as explants, which can achieve effective induction and rapid proliferation of callus, so as to solve the current shortage of Pine chrysalis medicinal resources

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Selection and disinfection of materials: choose the buds on the pine plants that grow healthy after falling leaves and have no damage by diseases and insect pests, put the buds on the ultra-clean workbench after peeling off the bud scales on the buds, and use a concentration of 70% Disinfect with alcohol for 30 seconds, then disinfect with 0.1% mercuric chloride for 15 minutes, and finally rinse with sterile water for 4 times and dry the water with sterilized filter paper;

[0015] (2) Induction culture of callus: Take the sterilized shoots, cut the leaf primordia on the shoots as explants and insert them into the callus induction medium MS+6-BA 1.0mg L -1 +NAA 0.3mg·L -1 +IAA 0.1mg·L -1 + sucrose 30g·L -1 +Agar 6.5g·L -1 The culture was carried out in medium, the pH value was 6.0, the culture temperature was 25°C, the light was 12 hours a day, and the light intensity was 1500lx. After 40 days of culture, the callus induction rate was 98%;

[0016] (3) Prolifera...

Embodiment 2

[0018] Change the callus induction medium in step (2) of Example 1 to MS+6-BA 2.0mg L -1 +NAA 0.5mg·L -1 +IAA0.3mg·L -1 + sucrose 30g·L -1 +Agar 6.5g·L -1 , other steps were the same as in Example 1, and the callus induction rate was 85% after the explants were cultured for 40 days.

Embodiment 3

[0020] Change the callus proliferation medium in step (3) of Example 1 to MS+6-BA 1.0 mg L -1 +IAA 0.5mg·L -1 + sucrose 30g·L -1 +Agar 6.5g·L -1 , the other steps were the same as in Example 1, and the callus proliferated 1.8 times after culturing for 40 days.

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Abstract

The invention discloses a method for culturing golden larch callus by taking phyllopodium as an explant. The method comprises the following steps of: disinfecting buds of the golden larch; cutting the phyllopodium on the buds after being disinfected and taking the phyllopodium as the explant; culturing by implanting the phyllopodium into a callus induced culture medium: MS+6-BA 1.0-2.0mg / L+NAA 0.1-1.0 mg / L+IAA 0.1-0.5 mg / L+ cane sugar 20-50 g / L+ agar 5.0-7.0 g / L and a multiplication culture medium: MS+6-BA 0.5-1.0mg / L+NAA 0.1-1.0 mg / L+ cane sugar 20-50 g / L+ agar 5.0-7.0 g / L or MS+6-BA 0.5-1.0mg / L+IAA 0.1-1.0 mg / L+ cane sugar 20-50 g / L+ agar 5.0-7.0 g / L; and finally realizing the effective induction and quick multiplication of the golden larch callus. According to the method provided by the invention, the present problem of being short of golden larch medicinal materials is effectively solved.

Description

technical field [0001] The invention relates to a method for tissue culture of pine plants, in particular to a method for culturing callus of pine plants with leaf primordia as explants. Background technique [0002] The golden pine is an ancient and relict species of Pinaceae endemic to my country. According to the new threatened species classification system standards of the World Conservation Union (IUCN) and the standards of the Chinese Plant Red Book, the golden pine is designated as a endangered species and listed as a national second-class protected plant. [0003] The golden pine is not only an important garden plant, but also an important medicinal plant with broad application prospects. Studies have found that the most important medicinal ingredient of pine pine is the diterpenoid compound hibiscus acid extracted from its bark and root bark. It is the anti-tumor effect that makes the medicinal value of jinsong more prominent. [0004] Modern pharmacological stud...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 王跃华杨小萍袁畅孙雁霞黄兴王丹王强蔡锐刘洪明李红梅王杰唐旭
Owner CHENGDU UNIV
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