Azoospermia related gene Fish detection method, related detection probe and kit

Abstract

The invention relates to an azoospermia related gene Fish detection method. The human chromosome show piece is subjected to conventional pretreatment, then the situ hybridization is carried out by using a detection probe, and the existence of the azoospermia related gene is judged according to the detected fluorescence signal, wherein the detection probe is preferentially a DAZ detection probe, and the DAZ is a nucleotide sequence with the serial number of AC053490.2 of the GeneBank in the NCBI. The invention further relates to a related detection probe and a kit. The azoospermia related gene Fish detection method and the related detection probe have ingenious design, are easy to operate, can be used for detecting the azoospermia related gene rapidly, accurately and intuitively, ensure the accurate and reliable detection result which can be used as the reference for the doctor to diagnose the azoospermia and can be popularized and applied widely. The specificity and the sensitivity of the azoospermia related gene Fish detection method and the related detection probe can be improved greatly.

Description

technical field [0001] The present invention relates to the technical field of disease-related gene detection, more particularly, to the technical field of azoospermia-related fusion gene detection, in particular to a Fish detection method for azoospermia-related genes, and related detection probes and kits. Background technique [0002] Severe oligospermia and azoospermia are one of the main causes of male infertility. Factors leading to severe oligospermia and azoospermia include abnormal chromosome number and structure, microbial infection, and drug effects. In recent years, a microdeletion phenomenon in the AZF region (spermatogenesis region) of the long arm of the Y chromosome has been found, which is associated with severe oligospermia and azoospermia. There are three subregions (AZFa, AZFb, AZFc) in the AZF region, and the sites contained in each subregion play different roles in different stages of male germ cell development. The deletion of the site can cause patie...

AI Technical Summary

Problems solved by technology

[0004] FISH is a new technology that combines molecular biology (probe) and cytogenetics (chromosome) to detect chromosomal abnormalities. It has been widely used as a fast, accurate and intuitive molecular genetics technology in b