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A hydrogel-encapsulated lentivirus Lingo-1 RNAi complex for repairing nerve injury and a preparation method thereof

A nerve injury and hydrogel technology, applied in nervous system diseases, drug combinations, pharmaceutical formulations, etc., can solve problems affecting the quality of life and burden of patients

Inactive Publication Date: 2013-12-04
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For these patients, even if the patient's life can be extended through treatment, the lifelong disability caused by spinal cord injury has brought a heavy burden to the family and society, and seriously affected the quality of life of the patient.

Method used

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  • A hydrogel-encapsulated lentivirus Lingo-1 RNAi complex for repairing nerve injury and a preparation method thereof
  • A hydrogel-encapsulated lentivirus Lingo-1 RNAi complex for repairing nerve injury and a preparation method thereof
  • A hydrogel-encapsulated lentivirus Lingo-1 RNAi complex for repairing nerve injury and a preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] Example 1 Preparation of hydrogel-encapsulated lentivirus Lingo-1 RNAi complex

[0043] (1) Construction of lentiviral Lingo-1 RNAi: For the construction method of lentiviral Lingo-1 RNAi, see Mi S, Miller RH, Lee X, et al. LINGO-1 negatively regulates meelination by oligodendrocytes. Nat Neurosci, 2005, 8( 6): 745-751. The green fluorescent protein (GFP)-labeled lentivirus is replaced by enhanced green fluorescent protein (EGFP) labeling;

[0044] (2) Preparation of hydrogel: prepare hydrogel and deionized water under sterile conditions to form a suspension with a mass ratio of 20% to 25%, and place the suspension at 2 to 8°C until it coagulates The gel was fully dissolved, and filtered and sterilized with a bottle cap filter with a pore size of 0.22 μm to obtain a hydrogel stock solution;

[0045] (3) Combine the lentivirus Lingo-1 RNAi constructed in step (1) and the hydrogel stock solution prepared in step (2) at 2-8°C to contain 1×10 7 The concentration of lentiv...

Embodiment 2

[0050] Example 2 Preparation of hydrogel-encapsulated lentivirus Lingo-1 RNAi complex

[0051] The method in this embodiment is the same as that described in Example 1, the only difference is that the hydrogel used in this embodiment is PNIPAm hydrogel.

Embodiment 3

[0052] Example 3 Hydrogel-encapsulated lentiviral Lingo-1 RNAi complex repair test for spinal cord injury

[0053] 1. Preparation of rat model with spinal cord transection injury

[0054] Female adult SD rats were intraperitoneally injected with 1% pentobarbital sodium (30mg / kg) for anesthesia, exposed T8-T10, removed the lamina on both sides, removed the T9 lamina, exposed the T10 spinal cord segment, and quickly removed it with a sharp-edged (No. 11) scalpel. The spinal cord was transected at a distance of 2 mm from the incision, and the spinal cord was transected again with a scalpel. The spinal cord tissue between the two incisions was gently clipped out with sharp forceps, and the bleeding was fully stopped. The wound was sutured layer by layer according to the order of the subcutaneous tissue of the muscle layer and the skin. Each animal was intramuscularly injected with penicillin 160,000 units 1ml / d gentamicin sulfate 250,000 units 0.5ml / d for 3 consecutive days with a...

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Abstract

The present invention discloses hydrogel-encapsulated lentivirus Lingo-1 RNAi complex for repairing nerve injuries. The complex adopts a strategy of combining Lingo-1 RNAi with a biological scaffold and a releasing system of the hydrogel, wherein, RNAi-interfered Lingo-1 coordinates with the hydrogel to promote axonal regeneration of spinal cord injuries, and therefore a new way to repair spinal cord injuries is provided. The present invention also discloses a preparation method of the hydrogel-encapsulated lentivirus Lingo-1 RNAi complex, having advantages of convenient operation and easy implementation. At the same time, the present invention additionally discloses the application of the hydrogel-encapsulated lentivirus Lingo-1 RNAi complex in preparing medicaments for the treatment of spinal cord injuries.

Description

technical field [0001] The invention relates to a complex of hydrogel and lentivirus Lingo-1 RNAi and a preparation method thereof, in particular to a hydrogel-wrapped lentivirus Lingo-1 RNAi complex and a preparation method thereof. Background technique [0002] According to reliable epidemiological statistics, there are approximately 250,000 spinal cord injury patients in the United States, with an annual increase of 11,000 new patients, and the annual new incidence rate is 4 / 100,000. Based on this calculation, there are at least 240,000 new spinal cord injury patients in the world every year. [0003] my country is a country with a large population in the world, and the number of spinal cord injury patients ranks first in the world. According to the preliminary statistics of the National Production Safety Committee of my country, 50,000 to 60,000 spinal cord injury patients are caused by production accidents every year, and as many as 70,000 to 80,000 spinal cord injury ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K48/00A61K9/06A61P25/28A61L31/16A61L31/14A61L31/06
Inventor 邓宇斌吴洪福万勇
Owner SUN YAT SEN UNIV