Method for synchronously detecting quantity and functions of specific thymus dependent lymph cells

Abstract

The invention discloses a method for synchronously detecting the quantity and functions of specific thymus dependent lymph cells, which can be used for synchronously and serially detecting the quantity of specific thymus dependent lymph cells aiming at specific antigens and reactivity thereof after receiving the stimulation of the antigens and has the advantages of high specificity, simplicity, convenience, easy application, no need of special instruments, great reduction of detection difficulty of specific T cells and easy popularization and application in general departments. According to the method, serial detection means that the specific T cells aiming at a series of epitopes, which are presented by a series of human major histocompatibility antigens can be synchronously detected via a micropore reaction plate, so that a detection kit aiming at target antigens not only can be used for covering most people, but also can be used for analyzing the specific T cells of various epitopes in one detection process. According to the method, the process of analyzing the immunologic function states of the specific cells of an organism becomes simple and convenient, so that the research and treatment of immunological diseases are promoted.

Description

technical field [0001] The invention belongs to the technical field of immunology, and relates to an antigen-specific thymus-dependent lymphocyte detection method. Background technique [0002] Antigen-specific T lymphocytes are the core cells that mediate adaptive immune responses, and play a vital role in anti-infection, anti-tumor, hypersensitivity and autoimmune diseases. Weakness directly reflects the functional state of the human body's specific cellular immunity, and has an important reference value different from that of antibodies in choosing treatment options, evaluating drug efficacy, predicting disease progression, and monitoring vaccine effects. However, compared with the detection of specific antibodies, the detection of the quantity and function of specific T cells is much more difficult, the methods are few and complicated, and the reagents are few and expensive. [0003] The specific recognition and combination of TCR (T cell antigen receptor) on the T cell...

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Problems solved by technology

[0009] So far, there is no knowledge of integrating pMHC tetramer technology, artificial antigen-presenting cell technology, ELISPOT technology, magnetic cell sorting technology and micro-well reaction plate to prepare magnetic bead-type artificial antigen-presenting cell micro-well reaction plate property rights, and there is no research report on detecting antigen-specific T cells using the scheme of the present invention